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英文学术期刊论文发表数据简介

   论文常用来指进行各个学术领域的研究和描述学术研究成果的文章，简称之为论文。它既是探讨问题进行学术研究的一种手段，又是描述学术研究成果进行学术交流的一种工具。论文发表是研究人员和学者为了分享研究成果并推动学术进步而进行的重要活动。在如今的学术界，发表论文已经成为衡量一个研究人员或学者成就的基本指标之一。   

   与此同时，学术文章需要经过同行评审，见刊在学术期刊上的文章通常涉及特定的学科。学术期刊展示了研究领域的成果，并起到了公示的作用。学术期刊分为普刊，专刊，核心期刊，国际英文刊。国际权威学术期刊出版商包括RELX Group、Springer-Verlag、Wiley & Sons Inc、SAGE Publishing、IEEE、MDPI等。

   为支持相关研究，CnOpenData推出英文学术期刊论文发表数据，包含多个数据源，其中，A数据源涵盖400+期刊目录和百万量级论文基本信息，包含期刊名称、国际标准期刊号（issn)、发行时间、影响因子、标题、作者、期卷号、DOI、摘要等字段。其他来源正在准备中，敬请期待。

时间区间

A来源：截至2023.07

B来源：截止2023.10

C来源：截止2023.11

字段展示

期刊基本信息字段表-A来源论文基本信息字段表-A来源
编号编号
期刊名称期刊名称
国际标准期刊号（issn)当前期卷号
发行时间类型
影响因子标题
引用得分作者
即将发表的文章期卷号
总发文量DOI
摘要
期刊基本信息字段表-B来源论文基本信息字段表-B来源
编号编号
期刊名称期刊名称
期刊简介期卷号
国际标准期刊号(ISSN)日期
一级分类标题
二级分类作者
学科领域DOI
引用得分页码
影响因子摘要
公开出版费用
收录-出版时限
摘要和索引
期刊基本信息字段表-C来源论文基本信息字段表-C来源
编号期刊名称
期刊名称年份
期刊链接期卷号
国际标准期刊号（ISSN）标题
期刊简介DOI
影响因子引用信息
近5年影响因子作者
出版日期
作者单位
页码
摘要

样本数据

期刊基本信息表-A来源

编号期刊名称国际标准期刊号（issn)发行时间影响因子引用得分即将发表的文章总发文量
1Acoustics2624-599X20192.148253
2Actuators2076-082520122.63.2121450
3Administrative Sciences2076-3387201133.910945
4Adolescents2673-70512021--9112
5Advances in Respiratory Medicine2543-603119091.8231493
6Aerobiology2813-50752023--11
7Aerospace2226-431020142.63342532
8Agriculture2077-047220113.63.6956489
9AgriEngineering2624-740220192.84.614305
10Agrochemicals2813-31452022--429
11Agronomy2073-439520113.75.210811397
12AI2673-26882020--8170
13Air2813-41682023--213
14Algorithms1999-489320082.33.7192607
15Allergies2313-57862021--146
16Alloys2674-063X2022--431
17Analytica2673-45322020--580
18Analytics2813-22032022--548
19Anatomia2813-05452022--442
20Anesthesia Research2813-58062023--22
21Animals2076-2615201134.212214036
22Antibiotics2079-638220124.85.5596203
23Antibodies2073-446820124.79.69488
24Antioxidants2076-3921201278.8798535
25Applied Biosciences2813-04642022--647
26Applied Mechanics2673-31612020-0.54199
27Applied Microbiology2673-80072021--18175
28Applied Nano2673-35012020--261
29Applied Sciences2076-341720112.74.540053636
30Applied System Innovation2571-557720183.84.98442
31AppliedChem2673-96232021--354
32AppliedMath2673-99092021--479
33Aquaculture Journal2673-94962021--238
34Architecture2673-89452021--474
35Arthropoda2813-33232023--312
36Arts2076-075220120.5-46877
37Astronomy2674-03462022--126
38Atmosphere2073-443320102.94.1518462
39Atoms2218-200420131.83.11777
40Audiology Research2039-434920111.70.610314
41Automation2673-40522020--269
42Axioms2075-1680201222.2462406
43Bacteria2674-13342022--131
44Batteries2313-0105201545.4181081
45Behavioral Sciences2076-328X20112.63362062
46Beverages2306-571020153.55.811605
47Big Data and Cognitive Computing2504-228920173.74.918519
48BioChem2673-64112021--145
49Bioengineering2306-535420144.64.2462524
50Biologics2673-84492021--252

论文基本信息表-A来源

编号期刊名称当前期卷号类型标题作者期卷号DOI摘要
29Applied Sciencesv.13(14), Jul 2023ArticleCache Misses and the Recovery of the Full AES 256 KeySamira Briongos / Pedro Malagón / Juan-Mariano de Goyeneche / Jose M. MoyaAppl. Sci. 2019, 9(5), 944;https://doi.org/10.3390/app9050944The CPU cache is a hardware element that leaks significant information about the software running on the CPU. Particularly, any application performing sequences of memory access that depend on sensitive information, such as private keys, is susceptible to suffer a cache attack, which would reveal this information. In most cases, side-channel cache attacks do not require any specific permission and just need access to a shared cache. This fact, combined with the spread of cloud computing, where the infrastructure is shared between different customers, has made these attacks quite popular. Traditionally, cache attacks against AES use the information about the victim to access an address. In contrast, we show that using non-access provides much more information and demonstrate that the power of cache attacks has been underestimated during these last years. This novel approach is applicable to existing attacks: <i>Prime+Probe</i>, <i>Flush+Reload</i>, <i>Flush+Flush</i> and <i>Prime+Abort</i>. In all cases, using cache misses as source of information, we could retrieve the 128-bit AES key with a reduction in the number of samples of between 93% and 98% compared to the traditional approach. Further, this attack was adapted and extended in what we call the encryption-by-decryption cache attack (EBD), to obtain a 256-bit AES key. In the best scenario, our approach obtained the 256 bits of the key of the OpenSSL AES T-table-based implementation using fewer than 10,000 samples, i.e., 135 milliseconds, proving that AES-256 is only about three times more complex to attack than AES-128 via cache attacks. Additionally, the proposed approach was successfully tested in a cross-VM scenario.
29Applied Sciencesv.13(14), Jul 2023ArticleMicrostructure and Mechanical Properties of Cement Mortar Containing Phase Change MaterialsHyun-Do Yun / Jong-Won Lee / Young-Il Jang / Seok-Joon Jang / Wonchang ChoiAppl. Sci. 2019, 9(5), 943;https://doi.org/10.3390/app9050943This paper presents an investigation of the characterization of cement mortar containing phase change materials (PCMs) in order to control the development of hydration heat. The study examined microstructural characteristics and properties of cement mortar with PCMs such as flow, compressive strength, and flexural strength. This research involved two types of PCM and up to 15% cement added to cement mortar mixtures. The two types of PCM used in this study are PCM with barium (PCM-Ba) and PCM with strontium (PCM-Sr). The experimental results indicate that both the incremental temperature rise and the maximum temperature release time of PCM up to 5% addition are delayed. Both PCM-Ba and PCM-Sr are effective in reducing the development of hydration heat. The microstructural analysis results show that the crystalloid content of cement mortar without PCMs is about 3% more from cement mortar with PCMs, regardless of the type of PCMs used, and that no significant difference is evident in the formation of crystals between cement mortar with and without PCMs.
29Applied Sciencesv.13(14), Jul 2023ArticleLandslide Susceptibility Mapping Based on Random Forest and Boosted Regression Tree Models, and a Comparison of Their PerformanceSoyoung Park / Jinsoo KimAppl. Sci. 2019, 9(5), 942;https://doi.org/10.3390/app9050942This study aims to analyze and compare landslide susceptibility at Woomyeon Mountain, South Korea, based on the random forest (RF) model and the boosted regression tree (BRT) model. Through the construction of a landslide inventory map, 140 landslide locations were found. Among these, 42 (30%) were reserved to validate the model after 98 (70%) had been selected at random for model training. Fourteen landslide explanatory variables related to topography, hydrology, and forestry factors were considered and selected, based on the results of information gain for the modeling. The results were evaluated and compared using the receiver operating characteristic curve and statistical indices. The analysis showed that the RF model was better than the BRT model. The RF model yielded higher specificity, overall accuracy, and kappa index than the BRT model. In addition, the RF model, with a prediction rate of 0.865, performed slightly better than the BRT model, which had a prediction rate of 0.851. These results indicate that the landslide susceptibility maps (LSMs) produced in this study had good performance for predicting the spatial landslide distribution in the study area. These LSMs could be helpful for establishing mitigation strategies and for land use planning.
29Applied Sciencesv.13(14), Jul 2023ArticleNumerical Analysis of Flow in Building Arrangement: Computational Domain DiscretizationMarcin Sosnowski / Renata Gnatowska / Karolina Grabowska / Jaros??aw Krzywański / Arkadiusz JamrozikAppl. Sci. 2019, 9(5), 941;https://doi.org/10.3390/app9050941The progress in environmental investigations such as the analysis of building arrangements in an urban environment could not have been expanded without the use of computational fluid dynamics (CFD) as a research tool. The rapid development of numerical models results in improved correlations to results obtained with real data. Unfortunately, the computational domain discretization is a crucial step in CFD analysis which significantly influences the accuracy of the generated results. Hence an innovative approach to computational domain discretization using polyhedral elements is proposed. The results are compared to commonly applied tetrahedral and hexahedral elements as well as experimental results of particle image velocimetry (PIV). The performed research proves that the proposed method is promising as it allows for the reduction of both the numerical diffusion of the mesh as well as the time cost of preparing the model for calculation. In consequence, the presented approach allows for better results in less time.
29Applied Sciencesv.13(14), Jul 2023ArticleClassification of Pulmonary CT Images by Using Hybrid 3D-Deep Convolutional Neural Network ArchitectureHuseyin Polat / Homay Danaei MehrAppl. Sci. 2019, 9(5), 940;https://doi.org/10.3390/app9050940Lung cancer is the most common cause of cancer-related deaths worldwide. Hence, the survival rate of patients can be increased by early diagnosis. Recently, machine learning methods on Computed Tomography (CT) images have been used in the diagnosis of lung cancer to accelerate the diagnosis process and assist physicians. However, in conventional machine learning techniques, using handcrafted feature extraction methods on CT images are complicated processes. Hence, deep learning as an effective area of machine learning methods by using automatic feature extraction methods could minimize the process of feature extraction. In this study, two Convolutional Neural Network (CNN)-based models were proposed as deep learning methods to diagnose lung cancer on lung CT images. To investigate the performance of the two proposed models (Straight 3D-CNN with conventional softmax and hybrid 3D-CNN with Radial Basis Function (RBF)-based SVM), the altered models of two-well known CNN architectures (3D-AlexNet and 3D-GoogleNet) were considered. Experimental results showed that the performance of the two proposed models surpassed 3D-AlexNet and 3D-GoogleNet. Furthermore, the proposed hybrid 3D-CNN with SVM achieved more satisfying results (91.81%, 88.53% and 91.91% for accuracy rate, sensitivity and precision respectively) compared to straight 3D-CNN with softmax in the diagnosis of lung cancer.
29Applied Sciencesv.13(14), Jul 2023ArticleAccuracy and Inter-Unit Reliability of Ultra-Wide-Band Tracking System in Indoor ExerciseAlejandro Bastida-Castillo / Carlos David Gómez-Carmona / Ernesto De la Cruz-Sánchez / Xavier Reche-Royo / Sergio José Ibá??ez / José Pino OrtegaAppl. Sci. 2019, 9(5), 939;https://doi.org/10.3390/app9050939The purpose of this study was to assess the accuracy of positional data and the inter-unit reliability of an ultra-wide-band (UWB) tracking system. Four well-trained males performed five courses designed for the analysis of x- and y-coordinate accuracy analysis, specifically related to the positional distance variation between the UWB data and the fixed reference lines of a basketball court. This was achieved using geographic information system (GIS) mapping software that calculated, for each interval and participant, the distance from the main axis of displacement and from the opposite side of the court each 0.5 s (x and y coordinate). The accuracy of the results was satisfactory, with a mean absolute error of all estimations for the x-position of 5.2 ± 3.1 cm and for the y-position of 5.8 ± 2.3 cm. Regarding inter-unit reliability, the intra-class correlation coefficient (ICC) value was high for the x-coordinate (0.65) and very high for the y-coordinate (0.85). The main findings of the study were: (i) The accuracy of UWB tracking systems can be considered suitable for practical applications in sport analyses; (ii) position estimations are very precise and acceptable for tactical analyses; (iii) the error of the position estimations does not change significantly across different courses; and (iv) the use of different devices does not significantly affect the measurement error.
29Applied Sciencesv.13(14), Jul 2023ArticleExploring the Utility of 3-D-printed Laboratory EquipmentYifei Zhou / Chuyun Duan / Iyll-Joon Doh / Euiwon BaeAppl. Sci. 2019, 9(5), 937;https://doi.org/10.3390/app9050937Many laboratories utilize different types of opto-mechanical positioning devices in their experiments. Such devices include lateral stages, which provide 1-dimenstional translational movement, 3-dimensional translation stages, and laboratory jacks, which provide a convenient way of changing the vertical position of a sample. Recent advances in and affordability of 3-D printing have opened up a variety of possibilities, not only providing versatile and custom-designed laboratory equipment but also reducing the cost of constructing typical laboratory opto-mechanical positioning stages. Here, we present the possibility of printing typical linear stages, thereby constructing a full XYZ stage. In addition, a vertical laboratory jack, which utilizes a scissor format, has also been printed using polylactic acid (PLA) filament. The design of these systems required modeling the strength of material to estimate the deflection, which was conducted by finite element analysis. The effectiveness of the proposed 3-D-printed positioning devices was tested by measuring the stroke and the repeatability. As an example of application, a multispectral reflection imaging device was constructed with the help of 3-D-printed linear stages and a laboratory scissor jack.
29Applied Sciencesv.13(14), Jul 2023ArticleThe Effect of Selected Additives on the Oil Uptake and Quality Parameters of Fried Instant NoodlesKatarzyna Marciniak-Lukasiak / Anna Zbikowska / Agata Marzec / Mariola KozlowskaAppl. Sci. 2019, 9(5), 936;https://doi.org/10.3390/app9050936The scope of the paper includes the analysis of various quality parameters of fried instant noodles depending on the type and amount of the additive added to the basic recipe. For the analyzed instant noodles, the effect of hydroxypropylmethylcellulose (HPMC), microcrystalline cellulose (MCG), maltodextrin, and psyllium on the quality parameters (oil uptake, moisture, water activity, color, and acoustics) were determined. Results show that the quality parameters of instant noodles significantly depend on the type and amount of additives. The addition of HPMC and MCG resulted in decrease absorption, while the use of maltodextrin and psyllium increased the fat absorbed during frying. There is a significant relationship between the type of additive and the color of instant noodles. Color brightening was observed for instant noodles with the HPMC and MCG, while the addition of maltodextrin and psyllium contributed in the darkening of instant noodles (reduction of the L* parameter). The type of additives significantly influenced the texture of the instant noodles. The samples with a 3% addition of maltodextrin had a softer texture than the control sample, while the instant noodles with the HPMC, MCG, and Psyllium were characterized by a harder texture. For instant noodles with the addition of HPMC, MCG, and Psyllium larger number of acoustic events and higher breaking force were observed than for the control one. The frying temperature significantly influenced the texture of analyzed instant noodles (acoustic and mechanical properties). Increasing frying temperature from 160–170 °C caused a significant increase in acoustic descriptors and force.
29Applied Sciencesv.13(14), Jul 2023ArticleMorphology-Controlled Versatile One-Pot Synthesis of Hydrophobic Gold Nanodots, Nanobars, Nanorods, and Nanowires and Their Applications in Surface-Enhanced Raman SpectroscopyJing Neng / Chen Xiang / Kan Jia / Xiaohua Nie / Peilong SunAppl. Sci. 2019, 9(5), 935;https://doi.org/10.3390/app9050935Many previously reported syntheses of gold nanoparticles required lengthy reaction times, complicated operations, high temperatures, or multi-step manipulations. In this work, a morphology-controlled versatile one-pot synthesis of hydrophobic gold nanodots, nanobars, nanorods, and nanowires has been developed. A series of gold nanomaterials ranging from round nanodots, short nanobars, and long nanorods to ultrathin and ultralong nanowires (diameter <2 nm, length >2 μm) have been readily prepared by simply adjusting the feeding ratio of chloroauric acid to oleylamine, oleic acid, and triphenylsilane. The silk-like ultralong and ultrathin nanowires were found to have a single crystalline structure and may have significant potential applications in microelectronics and biosensors. Large sizes of gold spherical nanoparticles were obtained from gold nanodots via a seed-mediated growth approach. These nanoparticles and ultralong nanowires showed excellent surface-enhanced Raman scattering (SERS) activity in organic solvents and, therefore, were employed as efficient organic-soluble SERS substrates for the detection of hydrophobic food toxicants, such as 3,4-benzopyrene, and carcinogens, such as benzidine.
29Applied Sciencesv.13(14), Jul 2023ReviewInvestigating the Crucial Role of Optic Flow in Postural Control: Central vs. Peripheral Visual FieldMilena Raffi / Alessandro PirasAppl. Sci. 2019, 9(5), 934;https://doi.org/10.3390/app9050934Optic flow stimuli are crucial for the control of stance in the upright position. The visual control of posture has recently received a lot of interest from several researchers. One of the most intriguing aspects is the contribution of the different parts of the visual field in the control of stance. Here we reviewed the results of several studies performed with different methodologies that tried to determine the effect of optic flow on postural control, by analyzing the role of the central and peripheral visual fields. Although the results were controversial, the majority of these studies agreed to assign the most important role in postural control to the peripheral retina. However, these studies were performed using different approaches and different definitions of the central and peripheral visual fields. The choice of the exact portion of the retina to be stimulated is crucial given that the stimulation of the central and the peripheral parts of the retina leads to the activation of different geniculo-cortical pathways and results in different cortical processing of information.
29Applied Sciencesv.13(14), Jul 2023ArticleLaboratory Observations of Repeated Interactions between Ruptures and the Fault Bend Prior to the Overall Stick-Slip Instability Based on a Digital Image Correlation MethodYan-Qun Zhuo / Yanshuang Guo / Sergei Alexandrovich BornyakovAppl. Sci. 2019, 9(5), 933;https://doi.org/10.3390/app9050933Fault geometry plays important roles in the evolution of earthquake ruptures. Experimental studies on the spatiotemporal evolution of the ruptures of a fault with geometric bands are important for understanding the effects of the fault bend on the seismogenic process. However, the spatial sampling of the traditional point contact type sensors is quite low, which is unable to observe the detailed spatiotemporal evolution of ruptures. In this study, we use a high-speed camera combined with a digital image correlation (DIC) method to observe ruptures during stick-slip motions of a simulated bent fault. Meanwhile, strain gages were also used to test the results of the DIC method. Multiple cycles of the alternative propagation of ruptures between the two fault segments on the both sides of the fault bend were observed prior to the overall failure of the fault. Moreover, the slip velocity and rupture speed were observed getting higher during this process. These results indicate the repeated interactions between the ruptures and the fault bend prior to the overall instability of the fault, which distinguishes the effect of the fault bend from the effect of asperities in straight faults on the evolution of ruptures. In addition, improvement in the temporal sampling rate of the DIC measurement system may further help to unveil the rupture evolution during the overall instability in future.
29Applied Sciencesv.13(14), Jul 2023ArticleImpact of Particle Size Distribution of Colloidal Particles on Contaminant Transport in Porous MediaJongmuk Won / Dongseop Lee / Khanh Pham / Hyobum Lee / Hangseok ChoiAppl. Sci. 2019, 9(5), 932;https://doi.org/10.3390/app9050932The presence of retained colloidal particles causes the retardation of contaminant transport when the contaminant is favorably adsorbed to colloidal particles. Although the particle size distribution affects the retention behavior of colloidal particles, the impact of particle size distribution on contaminant transport has not been reported to date. This study investigates the impact of the particle size distribution of the colloidal particles on contaminant transport through numerical simulation by representing the particle size distribution as a lognormal distribution function. In addition, the bed efficiency and contaminant saturation of simulated breakthrough curves were calculated, and a contaminant transport model with the Langmuir isotherm for the reaction between the contaminant–sand and contaminant–colloidal particle was introduced and validated with experimental data. The simulated breakthrough curves, bed efficiency, and contaminant saturation indicated that an increase in the mean and standard deviation of the particle size distribution causes the retardation of contaminant transport.
29Applied Sciencesv.13(14), Jul 2023ArticleA Comprehensive Feature Comparison Study of Open-Source Container Orchestration FrameworksEddy Truyen / Dimitri Van Landuyt / Davy Preuveneers / Bert Lagaisse / Wouter JoosenAppl. Sci. 2019, 9(5), 931;https://doi.org/10.3390/app9050931(1) Background: Container orchestration frameworks provide support for management of complex distributed applications. Different frameworks have emerged only recently, and they have been in constant evolution as new features are being introduced. This reality makes it difficult for practitioners and researchers to maintain a clear view of the technology space. (2) Methods: we present a descriptive feature comparison study of the three most prominent orchestration frameworks: Docker Swarm, Kubernetes, and Mesos, which can be combined with Marathon, Aurora or DC/OS. This study aims at (i) identifying the common and unique features of all frameworks, (ii) comparing these frameworks qualitatively and quantitatively with respect to genericity in terms of supported features, and (iii) investigating the maturity and stability of the frameworks as well as the pioneering nature of each framework by studying the historical evolution of the frameworks on GitHub. (3) Results: (i) we have identified 124 common features and 54 unique features that we divided into a taxonomy of 9 functional aspects and 27 functional sub-aspects. (ii) Kubernetes supports the highest number of accumulated common and unique features for all 9 functional aspects; however, no evidence has been found for significant differences in genericity with Docker Swarm and DC/OS. (iii) Very little feature deprecations have been found and 15 out of 27 sub-aspects have been identified as mature and stable. These are pioneered in descending order by Kubernetes, Mesos, and Marathon. (4) Conclusion: there is a broad and mature foundation that underpins all container orchestration frameworks. Likely areas for further evolution and innovation include system support for improved cluster security and container security, performance isolation of GPU, disk and network resources, and network plugin architectures.
29Applied Sciencesv.13(14), Jul 2023ArticleWet Snow Flashover Characteristics of 500-kV AC Insulator Strings with Different ArrangementsJingwei Xu / Fanghui Yin / Longji Li / Qingfeng Wen / Hao Wang / Shunnan Liu / Zhidong Jia / Masoud FarzanehAppl. Sci. 2019, 9(5), 930;https://doi.org/10.3390/app9050930In order to study the wet snow flashover characteristics of 500-kV AC insulator strings under different arrangements, wet snow flashover tests were carried out in the large climate chamber of China Electric Power Research Institute (CEPRI). The wet snow flashover voltages were obtained by the even-rising method and the flashovers were filmed by a camera. The test results showed that the installation of an anti-icing shed of large diameter could increase the wet snow flashover voltage. The distance between the two insulators was a key parameter that influenced the discharge process and the flashover voltage. Under Λ-string arrangement, for common insulators, the flashover performance of iced insulators increased with the connection angle; for anti-icing insulators, the flashover performance increased at first and then decreased with the connection angle. In wet snow conditions, when the connection angle was at the commonly adopted angle of 60°, the flashover performance of the common insulators under the V-string and Λ-string arrangements was almost the same. For anti-icing insulators, however, the V-string arrangement was recommended according to the tests. The results obtained in this study can provide a reference for external insulation design in wet snow conditions.
29Applied Sciencesv.13(14), Jul 2023ArticleThe Virial Effect—Applications for SF6 and CH4 Thermal PlasmasAndriniaina Harry Solo / Pierre Freton / Jean-Jacques GonzalezAppl. Sci. 2019, 9(5), 929;https://doi.org/10.3390/app9050929A tool based on the mass action law was developed to calculate plasma compositions and thermodynamic properties for pure gases and mixtures, assuming a local thermodynamic equilibrium for pressures of up to 300 bar. The collection of the data that was necessary for tool calculation was automated by another tool that was written using Python, and the formats for the model were adapted directly from the NIST and JANAF websites. In order to calculate the plasma compositions for high pressures, virial correction was introduced. The influences of the parameters that were chosen to calculate the Lennard–Jones (12-6) potential were studied. The results at high pressure show the importance of virial correction for low temperatures and the dependence of the dataset used. Experimental data are necessary to determine a good dataset, and to obtain interaction potential. However, the data available in the literature were not always provided, so they are not well-adapted to a large pressure range. Due to this lack, the formulation provided by L. I. Stiel and G. Thodos (Journal of Chemical and Engineering Data, vol. 7, 1962, p. 234–236) is a good alternative when the considered pressure is not close to the critical point. The results may depend strongly on the system studied: examples using SF<sub>6</sub> and CH<sub>4</sub> plasma compositions are given at high pressure.
29Applied Sciencesv.13(14), Jul 2023ArticleRobust Planning of Energy and Environment Systems through Introducing Traffic Sector with Cost Minimization and Emissions Abatement under Multiple UncertaintiesCong Chen / Xueting Zeng / Guohe Huang / Lei Yu / Yongping LiAppl. Sci. 2019, 9(5), 928;https://doi.org/10.3390/app9050928Motor vehicles have been identified as a growing contributor to air pollution, such that analyzing the traffic policies on energy and environment systems (EES) has become a main concern for governments. This study developed a dual robust stochastic fuzzy optimization—energy and environmental systems (DRSFO-EES) model for sustainable planning EES, while considering the traffic sector through integrating two-stage stochastic programming, robust two-stage stochastic optimization, fuzzy possibilistic programming, and robust fuzzy possibilistic programming methods into a framework, which can be used to effectively tackle fuzzy and stochastic uncertainties as well as their combinations, capture the associated risks from fuzzy and stochastic uncertainties, and thoroughly analyze the trade-offs between system costs and reliability. The proposed model can: (i) generate robust optimized solutions for energy allocation, coking processing, oil refining, heat processing, electricity generation, electricity power expansion, electricity importation, energy production, as well as emission mitigation under multiple uncertainties; (ii) explore the impacts of different vehicle policies on vehicular emission mitigation; (iii) identify the study of regional atmospheric pollution contributions of different energy activities. The proposed DRSFO-EES model was applied to the EES of the Beijing-Tianjin-Hebei (BTH) region in China. Results generated from the proposed model disclose that: (i) limitation of the number of light-duty passenger vehicles and heavy-duty trucks can effectively reduce vehicular emissions; (ii) an electric cars’ policy is enhanced by increasing the ratio of its power generated from renewable sources; and (iii) the air-pollutant emissions in the BTH region are expected to peak around 2030, because the energy mix of the study region would be transformed from one dominated by coal to one with a cleaner pattern. The DRSFO-EES model can not only provide scientific support for the sustainable managing of EES by cost-effective ways, but also analyze the desired policies for mitigating pollutant emissions impacts with a risk adverse attitude under multiple uncertainties.
29Applied Sciencesv.13(14), Jul 2023ArticleApplication of Sliding Rehabilitation Machine in Patients with Severe Cognitive Dysfunction after StrokeAe Ryoung Kim / Yang-Soo LeeAppl. Sci. 2019, 9(5), 927;https://doi.org/10.3390/app9050927A sliding rehabilitation machine (SRM) allows closed kinetic chain exercises of the hip, knee, and ankle. This study aimed to explore the feasibility of SRM training when included in an intensive rehabilitation program for post-stroke patients with severe cognitive dysfunction. The study design is a retrospective analysis. Patients who were admitted for inpatient rehabilitation after stroke with subsequent severe cognitive dysfunction were enrolled. Training with the SRM was conducted twice a day from Monday to Friday during hospitalization for three to four weeks. The number of sessions and the occurrence of side effects were documented daily. The SRM’s inclination angle, Berg Balance Scale (BBS), manual muscle test (MMT), and Korean version of the Modified Barthel Index (K-MBI) were documented upon admission and discharge. In 30 patients, 1736 sessions were performed from a total of 1754 scheduled sessions of SRM training. The performance rate was 98.9%, and there were no serious side effects. Transient side effects such as dizziness, nausea, and knee pain were observed in a few cases. At discharge, patients showed improvement in the SRM inclination angle, BBS, MMT, and K-MBI. This study shows that the use of the SRM for intensive muscle strengthening is readily applicable to patients who have had a stroke with severe cognitive dysfunction.
29Applied Sciencesv.13(14), Jul 2023ArticleA Transferable Prediction Approach for the Remaining Useful Life of Lithium-Ion Batteries Based on Small SamplesHaochen Qin / Xuexin Fan / Yaxiang Fan / Ruitian Wang / Qianyi Shang / Dong ZhangAppl. Sci. 2023, 13(14), 8498;https://doi.org/10.3390/app13148498Predicting the remaining useful life (RUL) of batteries can help users optimize battery management strategies for better usage planning. However, the RUL prediction accuracy of lithium-ion batteries will face challenges due to fewer data samples available for the new type of battery. This paper proposed a transferable prediction approach for the RUL of lithium-ion batteries based on small samples to reduce time in preparing battery aging data and improve prediction accuracy. This approach, based on improvements from the adaptive boosting algorithm, is called regression tree transfer adaptive boosting (RT-TrAdaBoost). It combines the advantages of ensemble learning and transfer learning and achieves high computational efficiency. The RT-TrAdaBoost approach takes the charging voltage and temperature curve as input and utilizes the classification and regression tree (CART) as the base learner, which has better feature capture ability. In the experiment, the working condition migration experiment and battery type migration experiment are conducted on non-overlapping datasets. The verified results revealed that the RT-TrAdaBoost approach could transfer not only the battery aging knowledge between various working conditions but also realize the RUL migration prediction from lithium iron phosphate battery to lithium cobalt oxide battery. The analysis of error and computation time demonstrates the proposed method’s high efficiency and speed.
29Applied Sciencesv.13(14), Jul 2023ArticleResearch on Multi-Sensor Simultaneous Localization and Mapping Technology for Complex Environment of Construction MachineryHaoling Ren / Yaping Zhao / Tianliang Lin / Jiangdong WuAppl. Sci. 2023, 13(14), 8496;https://doi.org/10.3390/app13148496Simultaneous localization and mapping (SLAM), as a key task of unmanned vehicles for construction machinery, is of great significance for later path planning and control. Construction tasks in the engineering field are mostly carried out in bridges, tunnels, open fields, etc. The prominent features of these environments are high scene similarity, few geometric features, and large-scale repetitive texture information, which is prone to sensor detection degradation. This leads to positioning drift and map building failure. The traditional method of motion estimation and 3D reconstruction uses a single sensor, which lacks enough information, has poor adaptability to the environment, and cannot guarantee good positioning accuracy and robustness in complex environments. Currently, the strategy of multi-sensor fusion is proven to be an effective solution and is widely studied. This paper proposes a SLAM framework that integrates LiDAR, IMU, and camera. It tightly couples the texture information observed by camera, the geometric information scanned by LiDAR, and the measured value of IMU, allowing visual-inertial odometry (VIO) and LiDAR-inertial odometry (LIO) common implementation. The LIO subsystem extracts point cloud features and matches them with the global map. The obtained pose estimation can be used for the initialization of the VIO subsystem. The VIO system uses direct method to minimize the photometric error and IMU measurement error between images to estimate the pose of the robot and the geometric structure of the scene. The two subsystems assist each other to perform pose estimation, and can operate normally even when any subsystem fails. A factor graph is used to combine all constraints to achieve global pose optimization. Keyframe and sliding window strategies are used to ensure real-time performance. Through real-vehicle testing, the system can perform incremental and real-time state estimation and reconstruct a dense 3D point cloud map, which can effectively solve the problems of positioning drift and mapping failure in the lack of geometric features or challenging construction environments.
29Applied Sciencesv.13(14), Jul 2023CommunicationReal-Time Information Fusion System Implementation Based on ARM-Based FPGAYu-Hsiang Tsai / Yung-Jhe Yan / Meng-Hsin Hsiao / Tzu-Yi Yu / Mang Ou-YangAppl. Sci. 2023, 13(14), 8497;https://doi.org/10.3390/app13148497In this study, an information fusion system displayed fusion information on a transparent display by considering the relationships among the display, background exhibit, and user’s gaze direction. We used an ARM-based field-programmable gate array (FPGA) to perform virtual–real fusion of this system as well as evaluated the virtual–real fusion execution speed. The ARM-based FPGA used Intel<sup>®</sup> Realsense<sup>TM</sup> D435i depth cameras to capture depth and color images of an observer and exhibit. The image data was received by the ARM side and fed to the FPGA side for real-time object detection. The FPGA accelerated the computation of the convolution neural networks to recognize observers and exhibits. In addition, a module performed by the FPGA was developed for rapid registration between the color and depth images. The module calculated the size and position of the information displayed on a transparent display according to the pixel coordinates and depth values of the human eye and exhibit. A personal computer with GPU RTX2060 performed information fusion in ~47 ms, whereas the ARM-based FPGA accomplished it in 25 ms. Thus, the fusion speed of the ARM-based FPGA was 1.8 times faster than on the computer.
29Applied Sciencesv.13(14), Jul 2023ArticleTwo Revised Deep Neural Networks and Their Applications in Quantitative Analysis Based on Near-Infrared SpectroscopyHong-Hua Huang / Jian-Fei Luo / Feng Gan / Philip K. HopkeAppl. Sci. 2023, 13(14), 8494;https://doi.org/10.3390/app13148494Small data sets make developing calibration models using deep neural networks difficult because it is easy to overfit the system. We developed two deep neural network architectures by revising two existing network architectures: the U-Net and the attention mechanism. The major changes were to use 1D convolutional layers to replace the fully connected layers. We also designed and combined average pooling and maximum pooling in our revised networks, respectively. We applied these revised network architectures to three publicly available data sets and the resulting calibration models can generate acceptable results for general quantitative analysis. It also generated rather good results for data sets that concern calibration transfer. It demonstrates that constructing network architectures by properly revising existing successful network architectures may provide additional choices in the exploration of the application of deep neural network in analytical chemistry.
29Applied Sciencesv.13(14), Jul 2023ArticleSemisupervised Speech Data Extraction from Basque Parliament Sessions and Validation on Fully Bilingual Basque–Spanish ASRMikel Penagarikano / Amparo Varona / Germán Bordel / Luis Javier Rodriguez-FuentesAppl. Sci. 2023, 13(14), 8492;https://doi.org/10.3390/app13148492In this paper, a semisupervised speech data extraction method is presented and applied to create a new dataset designed for the development of fully bilingual Automatic Speech Recognition (ASR) systems for Basque and Spanish. The dataset is drawn from an extensive collection of Basque Parliament plenary sessions containing frequent code switchings. Since session minutes are not exact, only the most reliable speech segments are kept for training. To that end, we use phonetic similarity scores between nominal and recognized phone sequences. The process starts with baseline acoustic models trained on generic out-of-domain data, then iteratively updates the models with the extracted data and applies the updated models to refine the training dataset until the observed improvement between two iterations becomes small enough. A development dataset, involving five plenary sessions not used for training, has been manually audited for tuning and evaluation purposes. Cross-validation experiments (with 20 random partitions) have been carried out on the development dataset, using the baseline and the iteratively updated models. On average, Word Error Rate (WER) reduces from 16.57% (baseline) to 4.41% (first iteration) and further to 4.02% (second iteration), which corresponds to relative WER reductions of 73.4% and 8.8%, respectively. When considering only Basque segments, WER reduces on average from 16.57% (baseline) to 5.51% (first iteration) and further to 5.13% (second iteration), which corresponds to relative WER reductions of 66.7% and 6.9%, respectively. As a result of this work, a new bilingual Basque–Spanish resource has been produced based on Basque Parliament sessions, including 998 h of training data (audio segments + transcriptions), a development set (17 h long) designed for tuning and evaluation under a cross-validation scheme and a fully bilingual trigram language model.
29Applied Sciencesv.13(14), Jul 2023ArticleFPGA-Based Methodology for Detecting Positional Accuracy Degradation in Industrial RobotsErvin Galan-Uribe / Luis Morales-Velazquez / Roque Alfredo Osornio-RiosAppl. Sci. 2023, 13(14), 8493;https://doi.org/10.3390/app13148493Industrial processes involving manipulator robots require accurate positioning and orienting for high-quality results. Any decrease in positional accuracy can result in resource wastage. Machine learning methodologies have been proposed to analyze failures and wear in electronic and mechanical components, affecting positional accuracy. These methods are typically implemented in software for offline analysis. In this regard, this work proposes a methodology for detecting a positional deviation in the robot’s joints and its implementation in a digital system of proprietary design based on a field-programmable gate array (FPGA) equipped with several developed intellectual property cores (IPcores). The method implemented in FPGA consists of the analysis of current signals from a UR5 robot using discrete wavelet transform (DWT), statistical indicators, and a neural network classifier. IPcores are developed and tested with synthetic current signals, and their effectiveness is validated using a real robot dataset. The results show that the system can classify the synthetic robot signals for joints two and three with 97% accuracy and the real robot signals for joints five and six with 100% accuracy. This system aims to be a high-speed reconfigurable tool to help detect robot precision degradation and implement timely maintenance strategies.
29Applied Sciencesv.13(14), Jul 2023ArticleSpectra Prediction for WLEDs with High TLCIHaiyang Wang / Peipei Wang / Zhiliang Jin / Yang Song / Daxi XiongAppl. Sci. 2023, 13(14), 8487;https://doi.org/10.3390/app13148487White light-emitting diodes (WLEDs) with a high television lighting consistency index (TLCI) are becoming popular in stadium, studio, and stage lighting, but they are hard to empirically manufacture because they exploit the response of a camera rather than that of the human eye. In this study, blue chips with green and red phosphors were used to build high-TLCI WLEDs, and a spectral prediction model for WLEDs used for calculating the TLCI was established. The model considers the reabsorption and re-emission of phosphors and spectral shift. To validate the model, WLEDs with a TLCI greater than 90, from 3000 K to 7000 K, were predicted and packaged. The maximum difference in the TLCI between the measured and predicted model values is approximately 1.93%. The spectral prediction model proposed in this study is expected to provide a helpful guideline for building high-TLCI WLEDs.
29Applied Sciencesv.13(14), Jul 2023ArticleOptimization of Growth Conditions for Magnetospirillum magnetotacticum and Green Synthesis of Metallic NanoparticlesRebekah Eleasa Sancho / Anushka Govindsamy / Karen PillayAppl. Sci. 2023, 13(14), 8491;https://doi.org/10.3390/app13148491Nanotechnology is especially useful in biotechnological and biomedical applications as nanomaterials have unique physicochemical properties. Current physical and chemical techniques used for the production of nanoparticles have various disadvantages that has led to the evaluation of biological strategies. This study focused on the use of a bacterial species known as <i>Magnetospirillum magnetotacticum</i> for the production of metallic nanoparticles. The cultivation of MTB is known to be tedious and time-consuming using the current standardized magnetic spirillum growth media (MSGM). This study explored the optimization of MSGM for improved growth and nanoparticle yield. It was found that glucose significantly improved and sustained the growth of <i>M. magnetotacticum</i> compared to other sole carbon sources having a sustainable OD of ~1.15. However, use of a higher concentration of sodium nitrate (40 mM) as a nitrogen source was able to significantly improve iron-containing nanoparticle yield by 1.6× with a final yield of 22 mg/50 mL when compared to the yield obtained from the MSGM original media. Growth media with a combination of glucose, sodium nitrate, ammonium sulphate and yeast extract showed the highest exponential growth of <i>Magnetospirillum magnetotacticum</i> compared to all other MSGM modifications with the highest OD being 1.7. Silver and gold nanoparticles were also successfully produced in addition to iron-containing nanoparticles. Overall, no direct correlation between growth and nanoparticle yield was found.
29Applied Sciencesv.13(14), Jul 2023ArticleInvestigation on the Stress and Deformation Evolution Laws of Shield Tunnelling through a Mining Tunnel StructureEntong Du / Lei Zhou / Ruizhen FeiAppl. Sci. 2023, 13(14), 8489;https://doi.org/10.3390/app13148489In the construction of a shield crossing an existing mined tunnel without load, it is imperative to develop corresponding design standards that reflect actual engineering force characteristics to ensure the successful completion of the tunnel construction. This study uses the MIDAS-GTS NX 2022 finite element software to facilitate the creation of a numerical model of a shield structure for an air-push-over mine tunnel project in Changsha, China while investigating the stress field’s evolution during shield construction and calculating the maximum positive and negative bending moments and maximum axial forces for different structures and other force states under various construction conditions. This study’s findings informed the design and construction optimisation of a shield tunnelling empty-push method. The outcomes of this numerical simulation led to several key findings: (1) The soil density exerted a significantly greater impact on the internal forces of the initial support structure than both the tunnel depth and soil Poisson’s ratio. Additionally, a sudden shift in internal forces occurred within the 300–350 mm range when the lining thickness was altered. (2) Factors such as the tunnel depth, soil density, soil Poisson’s ratio μ, and lining thickness similarly influenced the internal forces of the segment and the initial support. Notably, the backfill layer thickness significantly affected the segment’s maximum axial force, causing an abrupt change of approximately 300 mm. (3) It is essential to control the guide rail’s thickness under the shield machine equipment’s weight constraint to prevent it from becoming overly large.
29Applied Sciencesv.13(14), Jul 2023ArticleExploring the Effects of Low-Level Laser Therapy on the Cytocompatibility and Osteo/Odontogenic Potential of Gingival-Derived Mesenchymal Stem Cells: Preliminary ReportYaser A. Alhazmi / Mohammed Y. Aljabri / Shereen N. Raafat / Shaimaa M. Gomaa / Mohamed ShamelAppl. Sci. 2023, 13(14), 8490;https://doi.org/10.3390/app13148490Numerous tissue engineering uses for gingival-derived mesenchymal stem cells (GMSCs) have been demonstrated. Recently, low-level laser therapy (LLLT) has been projected as a factor that can improve MSCs’ regeneration capacity. Therefore, the aim of this research was to examine the impact of LLLT at 1.5 J/cm<sup>2</sup> and 3 J/cm<sup>2</sup> on the viability and osteo/odontogenic potential of GMSCs. An MTT assay was performed to detect viability. Osteo/odontogenic differentiation was evaluated using Alizarin Red S staining and qRT-PCR for the evaluation of the RUNX2, OC, DMP1, and DSPP genes. A two-way ANOVA with Tukey’s post hoc test was used to determine the statistical significance between groups. The results revealed that LLLT of both energy densities had no cytotoxic effect on GMSC viability. LLLT of 1.5 J/cm<sup>2</sup> demonstrated better viability than the higher energy density (3 J/cm<sup>2</sup>). Furthermore, the osteo/odontogenic differentiation potential was promoted following LLLT radiation, where both groups exhibited mineralized nodule formation, with the low-energy laser having a significantly higher Alizarin Red S stain level. A qRT-PCR analysis revealed higher expression levels of osteogenic and odontogenic markers in the LLLT groups compared to the control group. In conclusion, this study showed the potential application of LLLT as a non-toxic and effective strategy to enhance the regenerative capacity of GMSCs for tissue engineering and clinical treatments in the oral and craniofacial fields.
29Applied Sciencesv.13(14), Jul 2023ArticleA Perfect Decomposition Model for Analyzing Transportation Energy Consumption in ChinaYujie Yuan / Xiushan Jiang / Chun Sing LaiAppl. Sci. 2023, 13(7), 4179;https://doi.org/10.3390/app13074179Energy consumption in transportation industry is increasing. Transportation has become one of the fastest energy consumption industries. Transportation energy consumption variation and the main influencing factors of decomposition contribute to reduce transportation energy consumption and realize the sustainable development of transportation industry. This paper puts forwards an improved decomposition model according to the factors of change direction on the basis of the existing index decomposition methods. Transportation energy consumption influencing factors are quantitatively decomposed according to the transportation energy consumption decomposition model. The contribution of transportation turnover, transportation structure and transportation energy consumption intensity changes to transportation energy consumption variation is quantitatively calculated. Results show that there exists great energy-conservation potential about transportation structure adjustment, and transportation energy intensity is the main factor of energy conservation. The research achievements enrich the relevant theory of transportation energy consumption, and help to make the transportation energy development planning and carry out related policies.
29Applied Sciencesv.13(14), Jul 2023EditorialApplied Agri-Technologies for Agriculture 4.0—Part IDimitrios Kateris / Dionysis BochtisAppl. Sci. 2023, 13(7), 4180;https://doi.org/10.3390/app13074180
29Applied Sciencesv.13(14), Jul 2023ArticleUsing Historical Data to Dynamically Route Post-Disaster Assessment Unmanned Aerial Vehicles in the Context of Responding to TornadoesSean Grogan / Michel Gamache / Robert PellerinAppl. Sci. 2023, 13(7), 4178;https://doi.org/10.3390/app13074178Responding to tornado disasters resides at a unique intersection of search and rescue operations: it has attributes of wilderness and maritime search and rescue operations and search and rescue operations in the aftermath of earthquakes and hurricanes. This paper presents a method of attempting to leverage historical data to more efficiently identify the extent of the area damaged by a tornado. To assist in building and understanding the historical data, we also develop a method to generate tornado areas that react similarly to the limited historical data set. The paper successfully demonstrates the method of creating artificial tornado instances that can be used as a testing sandbox for the further development of tools when responding to tornado-type disasters. These artificial instances perform similarly in some important metrics to the historical database of tornado instances that we produced. This paper also shows that the use of historical tornado trends has an impact on the response method outlined in this article, typically reducing the standard deviation of the time it takes to fully identify the extent of the damage.
29Applied Sciencesv.13(14), Jul 2023ArticleMorphological Integration on the Calcaneum of Domestic Sheep (Ovis aries Linnaeus, 1758)—A Geometric Morphometric StudyPere Miquel Parés-Casanova / Carme Rissech / Simon Davis / Lluís LloverasAppl. Sci. 2023, 13(7), 4177;https://doi.org/10.3390/app13074177Morphological integration and modularity refer to the degree of covariation between the different components of an anatomical structure. Modularity refers to structures that have components which covary strongly, but at the same time are relatively independent. Integration refers to the coordinated variation of the components of a functional anatomical structure. The hindlimb basipodium is a complex functional structure. The integration of its parts must arise from a coordinated development and functionality. Our objective in this study was to study the levels of integration of two modules on the calcaneum in domestic sheep. The calcaneum develops from two different centres. One gives rise to the body and its process while the other gives rise to the distal half of the bone. The hypothesis of modularity of two parts of the calcaneum was tested using the Escoufier RV coefficient and an analysis of two blocks of Partial Least Squares. These allowed us to evaluate the level of morphological integration. For this purpose, digital images of the medial aspect of complete calcanea of 47 domestic sheep (<i>Ovis aries</i>) were used. Twenty 2D coordinates of homologous anatomical landmarks (4 for the body and the process and 16 for the distal half of the bone) were collected as morphometric data. These were studied using geometric morphometrics. The results indicated good evidence of modular organization and a medium morphological integration between the two modules. Thus, according to our results, the two modules seem to exist and behave in a rather independent way. They appear to share functions rather than precursors of development. This study is important in order to elucidate the underlying factors in the processes of the development of the sheep calcaneum.
29Applied Sciencesv.13(14), Jul 2023ArticleAnalytical Method and Analysis of Cold-Joint InterfaceJuozas Mas??nas / Remigijus ??alna / Linas Juknevi??ius / Juozas ValivonisAppl. Sci. 2023, 13(7), 4176;https://doi.org/10.3390/app13074176The behaviour of the interface between two concrete layers, subjected to shear, is a complex process that is influenced by many different parameters. Knowledge of concrete interface performance is insufficient to this day. Most of the existing analytical methods are only suitable for determining the highest interface shear resistance and do not consider the interface behaviour at other stages. This article focuses on smooth concrete interfaces, which have their layers cast at different times (cold-joint interface). By analysing the results of different experimental push-off tests, presented in the literature, a novel analytical method was developed for the previously described concrete interface. Furthermore, numerical models of push-off tests were developed. A parametric numerical analysis was employed to determine the influence of various physical parameters that might affect interface shear behaviour. Most importantly, the results acquired using the proposed analytical method were compared with the findings of numerical analysis and experimental results acquired by other authors. The values of shear stress and slippage were found to be relatively close. Therefore, the proposed analytical method is suitable for the analysis of smooth concrete interfaces cast at different times.
29Applied Sciencesv.13(14), Jul 2023ArticleDevice Orientation Independent Human Activity Recognition Model for Patient Monitoring Based on Triaxial AccelerationSara Caramaschi / Gabriele B. Papini / Enrico G. CaianiAppl. Sci. 2023, 13(7), 4175;https://doi.org/10.3390/app13074175Tracking a person’s activities is relevant in a variety of contexts, from health and group-specific assessments, such as elderly care, to fitness tracking and human–computer interaction. In a clinical context, sensor-based activity tracking could help monitor patients’ progress or deterioration during their hospitalization time. However, during routine hospital care, devices could face displacements in their position and orientation caused by incorrect device application, patients’ physical peculiarities, or patients’ day-to-day free movement. These aspects can significantly reduce algorithms’ performances. In this work, we investigated how shifts in orientation could impact Human Activity Recognition (HAR) classification. To reach this purpose, we propose an HAR model based on a single three-axis accelerometer that can be located anywhere on the participant’s trunk, capable of recognizing activities from multiple movement patterns, and, thanks to data augmentation, can deal with device displacement. Developed models were trained and validated using acceleration measurements acquired in fifteen participants, and tested on twenty-four participants, of which twenty were from a different study protocol for external validation. The obtained results highlight the impact of changes in device orientation on a HAR algorithm and the potential of simple wearable sensor data augmentation for tackling this challenge. When applying small rotations (<20 degrees), the error of the baseline non-augmented model steeply increased. On the contrary, even when considering rotations ranging from 0 to 180 along the frontal axis, our model reached a f1-score of <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mn>0.85</mn><mo>±</mo><mn>0.11</mn></mrow></semantics></math></inline-formula> against a baseline model f1-score equal to <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mn>0.49</mn><mo>±</mo><mn>0.12</mn></mrow></semantics></math></inline-formula>.
29Applied Sciencesv.13(14), Jul 2023ArticleA Method for Managing Software Assets in the Automotive Industry (Focusing on the Case of Hyundai Motor Company and Parts Makers)Changhan Ryu / Sungryong DoAppl. Sci. 2023, 13(7), 4174;https://doi.org/10.3390/app13074174We propose a method for managing software assets in the automotive industry to enhance software competitiveness and to reduce development costs. The ownership of software assets in the automotive industry is held by automotive parts companies, making it challenging to exchange these technologies. Moreover, the criteria for determining software assets are often unclear, resulting in difficulties in integrating automotive software and implementing over-the-air updates. To address these issues, we suggest breaking down black-boxed software assets into tradable components, valuating them, and introducing the concept of exchanging software technology assets. Additionally, we provide a structured approach for recycling used software assets and establish a software asset management system for registration and tracking. Our proposed approach can help traditional automotive OEMs narrow the technology gap with automakers such as Tesla and improve their software competitiveness in the automotive industry. This paper contributes to the advancement of software asset management practices in the automotive industry, and provides insights into the integration of automotive software and over-the-air updates.
29Applied Sciencesv.13(14), Jul 2023ArticleA Non-Destructive Method for Predicting Critical Load, Critical Thickness and Service Life for Corroded Spherical Shells under Uniform External Pressure Based on NDT DataCheng Huijuan Liu / Giuseppe LacidognaAppl. Sci. 2023, 13(7), 4172;https://doi.org/10.3390/app13074172A pressurized spherical shell that is continuously corroded will likely buckle and lose its stability. There are many analytical and numerical methods to study this problem (critical load, critical thickness, and service life), but the friendliness (operability) in engineering test applications is still not ideal. Therefore, in this paper, we propose a new non-destructive method by combining the Southwell non-destructive procedure with the stable analysis method of corroded spherical thin shells. When used carefully, it can estimate the critical load (critical thickness) and service life of these thin shells. Furthermore, its procedure proved to be more practical than existing methods; it can be easily mastered, applied, and generalized in most engineering tests. When used properly, its accuracy is acceptable in the field of engineering estimations. In the context of the high demand for non-destructive analysis in industry, it may be of sufficient potential value to be used as a reference for existing estimating methods based on NDT data.
29Applied Sciencesv.13(14), Jul 2023ArticleSystem Architecture Design of IoT-Based Smart CitiesBedir Tekinerdogan / ??mer K??ksal / Turgay ??elikAppl. Sci. 2023, 13(7), 4173;https://doi.org/10.3390/app13074173The integration of Internet of Things (IoT) networks into smart cities is crucial to enhance the efficiency of city operations and services. Designing a smart city architecture that can adapt to the constantly changing functional and quality requirements of city services is essential. However, critical decisions must be made during this process, such as selecting communication protocols, ensuring security and safety, optimizing time performance, and processing data capacity. To address these challenges, this paper proposes a systematic approach to guide the system architecture design of IoT-based smart cities. The approach starts with feature-driven domain analysis to model smart city requirements, followed by the design of a reference architecture for IoT-based smart cities. The architecture is modeled using selected architectural views, while considering key stakeholders and their concerns. Additionally, this paper presents valuable insights into lessons learned and challenges encountered during the process of creating IoT-based smart cities. This information can assist practitioners in developing such smart cities and pave the way for future research in this field. By following this proposed approach, smart city architects can design a robust and adaptable system architecture that can meet the evolving needs of smart city services.
29Applied Sciencesv.13(14), Jul 2023ArticlePossibilities of Detecting Damage Due to Osmosis of GFRP Composites Used in Marine ApplicationsWaldemar Swiderski / Martyna StragAppl. Sci. 2023, 13(7), 4171;https://doi.org/10.3390/app13074171The marine composites market is driven by the increasing demand for lightweight, corrosion-resistant, and impact-resistant boats. Polymer matrix composites are currently the most popular composite material in marine applications. Fiberglass composites are practically the main type of fiber composites that are used extensively in marine applications. Due to the aggressive sea environment, composite structural elements of ships are exposed to damage due to the phenomenon of osmosis. This damage is also favored by defects that result from impacts and technological errors during the production of these elements. Non-destructive testing methods are necessary to detect damage in the internal structure of the composite. The paper presents a numerical analysis of the possibility of using vibrothermography in the detection of defects in glass–fiber reinforced laminates in marine applications. Numerical simulations have shown that the most favorable method for detecting defects will be acoustic waves. This is an unusual application because, as a rule, the range of ultrasonic waves is used in vibrothermography. In our further works, it is planned to verify numerical calculations through experimental research. The applicability of the terahertz technique was also assessed. During the experimental testing, all defects in the test sample of the glass–fiber reinforced composite were detected using this technique. The presented results indicate the applicability of the presented methods for the detection of defects in composites used in marine applications.
29Applied Sciencesv.13(14), Jul 2023ArticleDetection of Additives with the Help of Discrete Geometrical InvariantsRaoul Nigmatullin / Artem Vorobev / Herman Budnikov / Artem Sidelnikov / Elza MaksyutovaAppl. Sci. 2019, 9(5), 926;https://doi.org/10.3390/app9050926In this paper, we propose a general mathematical method for the detection of electrochemical additives in a given solute with the help of discrete geometrical invariants (DGI). This idea is based on the generalization of Pythagor’s theorem that can be proved for two random sets located in the two-dimensional (2D) plane. This statement follows from the previous ideas proposed by Babenko, who essentially modernized the well-known theorem and propagated it on a wide class of “right” discrete sets with different symmetry. However, attentive analysis of these results shows that there is a possibility for their further generalization. For practical purposes, it is important to have discrete and deterministic curve(s) with the limited number of parameters that enables comparing two <i>random</i> sets of <i>any</i> nature if their quantitative description expressed in terms of the “best-fit” model is <i>absent</i>. Under the best-fit model, we imply the microscopic model that enables describing the measured data in terms of the minimal set of the fitting parameters. We propose at least two invariants: (a) the curve of the second order that coincides with the classical ellipse oriented at an arbitrary direction relative to the <i>X</i>- and <i>Y</i>-axes, and (b) the curve of the fourth order that has eight quantitative parameters and includes the cross-combination of the integer moments. In this paper, the DGIs of both types were used. These curves are made useful for the solution of a key problem in electrochemistry, i.e., the detection of small concentrations of <span style="font-variant: small-caps;">d</span>-tryptophan (6.54 ÷ 38.7) 10<sup>−5</sup> mol·L<sup>−1</sup> in a given solute (phosphate buffer solution (Na<sub>2</sub>HPO<sub>4</sub> + KH<sub>2</sub>PO<sub>4</sub>) with pH = 6.86) that was activated by electrodes of two types—Pt (platinum) and C (carbon). The DGI method is free from treatment errors and model suppositions; therefore, it can be applied for the detection of small additives in a given solute and a further description can be attained with the help of a monotone/calibration curve expressed by means of parameters associated with the DGI.
29Applied Sciencesv.13(14), Jul 2023ArticleA Quantitative Index to Evaluate the Commutation Failure Probability of LCC-HVDC with a Synchronous CondenserJiangbo Sha / Chunyi Guo / Atiq Ur Rehman / Chengyong ZhaoAppl. Sci. 2019, 9(5), 925;https://doi.org/10.3390/app9050925Since thyristor cannot turn off automatically, line commutated converter based high voltage direct current (LCC-HVDC) will inevitably fail to commutate and therefore auxiliary controls or voltage control devices are needed to improve the commutation failure immunity of the LCC-HVDC system. The voltage control device, a synchronous condenser (SC), can effectively suppress the commutation failure of the LCC-HVDC system. However, there is a need for a proper evaluation index that can quantitatively assess the ability of the LCC-HVDC system to resist the occurrence of commutation failures. At present, the main quantitative evaluation indicators include the commutation failure immunity index and the commutation failure probability index. Although they can reflect the resistance of the LCC-HVDC system to commutation failures to a certain extent, they are all based on specific working conditions and cannot comprehensively evaluate the impact of SCs on suppressing the commutation failure of the LCC-HVDC system under certain fault ranges. In order to more comprehensively and quantitatively evaluate the influence of SCs on the commutation failure susceptibility of the LCC-HVDC system under certain fault ranges, this paper proposes the area ratio of commutation failure probability. The accuracy of this new index was verified through the PSCAD/EMTDC. Based on the CIGRE benchmark model, the effects of different synchronous condensers on LCC-HVDC commutation failure were analyzed. The results showed that the new index could effectively and more precisely evaluate the effect of SCs on commutation failures. Moreover, the proposed index could provide a theoretical basis for the capacity allocation of SCs in practical projects and it could also be utilized for evaluating the impact of other dynamic reactive power compensators on the commutation failure probability of the LCC-HVDC system under certain fault ranges.
29Applied Sciencesv.13(14), Jul 2023ArticleMulti-Robot Trajectory Planning and Position/Force Coordination Control in Complex Welding TasksYahui Gan / Jinjun Duan / Ming Chen / Xianzhong DaiAppl. Sci. 2019, 9(5), 924;https://doi.org/10.3390/app9050924In this paper, the trajectory planning and position/force coordination control of multi-robot systems during the welding process are discussed. Trajectory planning is the basis of the position/ force cooperative control, an object-oriented hierarchical planning control strategy is adopted firstly, which has the ability to solve the problem of complex coordinate transformation, welding process requirement and constraints, etc. Furthermore, a new symmetrical internal and external adaptive variable impedance control is proposed for position/force tracking of multi-robot cooperative manipulators. Based on this control approach, the multi-robot cooperative manipulator is able to track a dynamic desired force and compensate for the unknown trajectory deviations, which result from external disturbances and calibration errors. In the end, the developed control scheme is experimentally tested on a multi-robot setup which is composed of three ESTUN industrial manipulators by welding a pipe-contact-pipe object. The simulations and experimental results are strongly proved that the proposed approach can finish the welding task smoothly and achieve a good position/force tracking performance.
29Applied Sciencesv.13(14), Jul 2023ArticleCPES Testing with mosaik: Co-Simulation Planning, Execution and AnalysisCornelius Steinbrink / Marita Blank-Babazadeh / André El-Ama / Stefanie Holly / Bengt Lüers / Marvin Nebel-Wenner / Rebeca P. Ramírez Acosta / Thomas Raub / Jan S??ren Schwarz / Sanja Stark / Astrid Nie??e / Sebastian LehnhoffAppl. Sci. 2019, 9(5), 923;https://doi.org/10.3390/app9050923The complex nature of cyber-physical energy systems (CPES) makes systematic testing of new technologies for these setups challenging. Co-simulation has been identified as an efficient and flexible test approach that allows consideration of interdisciplinary dynamic interactions. However, basic coupling of simulation models alone fails to account for many of the challenges of simulation-based multi-domain testing such as expert collaboration in test planning. This paper illustrates an extended CPES test environment based on the co-simulation framework <span style="font-variant: small-caps;">mosaik</span>. The environment contains capabilities for simulation planning, uncertainty quantification and the development of multi-agent systems. An application case involving virtual power plant control is used to demonstrate the platform’s features. Future extensibility of the highly modular test environment is outlined.
29Applied Sciencesv.13(14), Jul 2023ArticleImpact of Wind Veer and the Coriolis Force for an Idealized Farm to Farm Interaction CaseOla Eriksson / Simon-Philippe Breton / Karl Nilsson / Stefan IvanellAppl. Sci. 2019, 9(5), 922;https://doi.org/10.3390/app9050922The impact of the Coriolis force on the long distance wake behind wind farms is investigated using Large Eddy Simulations (LES) combined with a Forced Boundary Layer (FBL) technique. When using the FBL technique any mean wind shear and turbulent fluctuations can be added with body forces. The wind shear can also include the mean wind veer due to the Coriolis force. The variation of the Coriolis force due to local deviations from the mean profile, e.g., from wakes, is not taken into account in the FBL. This can be corrected for with an extra source term in the equations, hereon defined as the Coriolis correction. For a row of 4 turbines it is shown that the inclusion of the wind veer turns the wake to the right, while including the Coriolis correction turns it to the left. When including both wind veer and Coriolis correction the impact of wind veer dominates. For an idealized farm to farm interaction case, two farms of <inline-formula> <math display="inline"> <semantics> <mrow> <mn>4</mn> <mo>∗</mo> <mn>4</mn> </mrow> </semantics> </math> </inline-formula> turbines with 6 km in between, it can be seen that when including wind veer and the Coriolis correction a approximately 3% increase in the relative production for a full wake direction can be seen and only a slightly smaller increase can be seen when including only wind veer. The results indicate that FBL can be used for studies of long distance wakes without including a Coriolis correction but efforts need to be taken to use a wind shear with a correct mean wind veer.
29Applied Sciencesv.13(14), Jul 2023ArticleThe Optimization of Cold Rubidium Atom Two-Photon Transition Excitation with an Erbium-Fiber Optical Frequency CombHaoyuan Lu / Jianxiao Leng / Jianye ZhaoAppl. Sci. 2019, 9(5), 921;https://doi.org/10.3390/app9050921We demonstrated the observation of cold rubidium atom two-photon transition excitation by a fiber optical frequency comb. In addition to this, we optimized the repetition rate of optical frequency comb to enhance two-photon intensity by controlling cavity length and pump source of optical comb. This technique can fine tune repetition rate to corresponding stepwise two-photon transition resonance frequency and improve the transition intensity by three times. This method is useful in Doppler laser cooling and detection of macromolecules.
29Applied Sciencesv.13(14), Jul 2023ArticleHolographic Element-Based Effective Perspective Image Segmentation and Mosaicking Holographic Stereogram PrintingFan Fan / Xiaoyu Jiang / Xingpeng Yan / Jun Wen / Song Chen / Teng Zhang / Chao HanAppl. Sci. 2019, 9(5), 920;https://doi.org/10.3390/app9050920Effective perspective image segmentation and mosaicking (EPISM) method is an effective holographic stereogram printing method, but a mosaic misplacement of reconstruction image occurred when focusing away from the reconstruction image plane. In this paper, a method known as holographic element-based effective perspective image segmentation and mosaicking is proposed. Holographic element (hogel) correspondence is used in EPISM method as pixel correspondence is used in direct-writing digital holography (DWDH) method to generate effective perspective images segments. The synthetic perspective image for holographic stereogram printing is obtained by mosaicking all the effective perspective images segments. Optical experiments verified that the holographic stereogram printed by the proposed method can provide high-quality reconstruction imagery and solve the mosaic misplacement inherent in the EPISM method.
29Applied Sciencesv.13(14), Jul 2023ArticleDesigning a Waterless Toilet Prototype for Reusable Energy Using a User-Centered Approach and InterviewsHyun-Kyung LeeAppl. Sci. 2019, 9(5), 919;https://doi.org/10.3390/app9050919User-oriented community engagement can reveal insights into ways of improving a community and solving complex public issues, such as natural resource scarcity. This study describes the early process of co-designing a novel, waterless toilet to respond to the water scarcity problem in the Republic of Korea. It presents how we designed a toilet focusing on three factors—a sanitization function, an ergonomic posture, and clean aesthetics—by conducting focus group interviews as part of a user engagement approach to understand what community users want from a toilet and ways of improving their toilet experiences. The results not only supported the development of an experiential service design project to raise community awareness of water scarcity but also supported scientists and engineers in experimenting with and developing new technologies by collaborating closely with designers.
29Applied Sciencesv.13(14), Jul 2023ArticleLifetime Assessment and Optimization of a Welded A-Type Frame in a Mining Truck Considering Uncertainties of Material Properties and Structural Geometry and LoadChengji Mi / Wentai Li / Xuewen Xiao / Haigen Jian / Zhengqi Gu / Filippo BertoAppl. Sci. 2019, 9(5), 918;https://doi.org/10.3390/app9050918In order to improve the fatigue performance of a welded A-type frame in a heavy off-road mining truck, a novel method was presented to implement lifetime and weight collaborative optimization while considering uncertainties in geometry dimension, material properties, and bearing load. The mechanical and cyclic material parameters were obtained from experimental work to characterize the base metal and the weldment. The finite element model of a welded A-type frame was constructed to analyze stress distribution and predict fatigue life, the force time histories of which were acquired from multi-body dynamics simulation. The simulated failure position and fatigue life had a good agreement with the actual results. Then, both structural lifetime and weight were considered as optimization objectives. The thickness of main steel plates and elastic and cyclic material parameters were chosen as uncertain design variables as well as main loads at connection locations. The fifty sample points in the light of Latin hypercube sampling method and its responses calculated by finite element analysis were supposed to build the approximation model based on the Kriging approximation method. After its fitting precision was guaranteed, the non-dominated sorting genetic algorithm II (NSGA-II) was utilized to find the optimal solution. Finally, the fatigue life of a welded A-type frame was increased to 2.40 × 10<sup>5</sup> cycles and its mass was lessened by 8.2%. The optimized results implied that good fatigue performance of this welded A-type frame needs better welding quality, lower running speed for downhill and turning road surface, and thicker front plates.
29Applied Sciencesv.13(14), Jul 2023ArticleThermal Analysis of Cornea Heated with Terahertz RadiationWenquan Liu / Yuanfu Lu / Rongbin She / Guanglu Wei / Guohua Jiao / Jiancheng Lv / Guangyuan LiAppl. Sci. 2019, 9(5), 917;https://doi.org/10.3390/app9050917We numerically investigate the thermal effects in a cornea illuminated by terahertz radiation. By modifying the bioheat and Arrhenius equations, we studied the heat-transfer and temperature distributions in the corneal tissue, and evaluated the potential thermal damage. The influence of the beam radius and power density are discussed. We also estimated the effective cornea-collagen shrinkage region, and evaluated the degree of thermal damage in the cornea. We expect this work to open up a novel effective and safe thermal-treatment approach based on THz radiation for cornea reshaping in the field of ophthalmology.
29Applied Sciencesv.13(14), Jul 2023ArticlePotential of Fluorescence Index Derived from the Slope Characteristics of Laser-Induced Chlorophyll Fluorescence Spectrum for Rice Leaf Nitrogen Concentration EstimationJian Yang / Lin Du / Shuo Shi / Wei Gong / Jia Sun / Biwu ChenAppl. Sci. 2019, 9(5), 916;https://doi.org/10.3390/app9050916Leaf nitrogen concentration (LNC) is a major biochemical parameter for estimating photosynthetic efficiency and crop yields. Laser-induced fluorescence, which is a promising potential technology, has been widely used to estimate the growth status of crops with the help of multivariate analysis. In this study, a fluorescence index was proposed based on the slope characteristics of fluorescence spectrum and was used to estimate LNC. Then, the performance of different fluorescence characteristics (proposed fluorescence index, fluorescence ratios, and fluorescence characteristics calculated by principal component analysis (PCA)) for LNC estimation was analyzed based on back-propagation neural network (BPNN) model. The proposed fluorescence index exhibited more stability and reliability for LNC estimation than fluorescence ratios and characteristics calculated by PCA. In addition, the effect of different kernel functions and hidden layer sizes of BPNN model on the accuracy of LNC estimation was discussed for different fluorescence characteristics. The optimal train functions “trainrp,” “trainbr,” and “trainlm” were then selected with higher <i>R</i><sup>2</sup> and lower standard deviation (SD) values than those of other train functions. In addition, experimental results demonstrated that the hidden layer size has a smaller impact on the accuracy of LNC estimation than the kernel function of the BPNN model.
29Applied Sciencesv.13(14), Jul 2023ArticleRemote Thermoelastic Characterization of Candidate Structural and Protective Coatings for Lead-Bismuth Eutectic Cooled Nuclear ReactorsBert Verstraeten / Jan Sermeus / Tom Van der Donck / Paul Schuurmans / Christ GlorieuxAppl. Sci. 2019, 9(5), 915;https://doi.org/10.3390/app9050915A laser ultrasonics approach to remotely characterize the elastic and thermal properties of a coating-substrate structure, used to protect fuel claddings in lead-bismuth eutectic cooled nuclear reactors, is presented and validated with experimental results. A transient grating geometry is used for multiple wavelength excitation of thermoelastic displacements, and laser beam deflection for detection. The value of Young’s modulus of the coating layer as calculated from the coating’s Rayleigh velocity of 190 ± 50 GPa is in accordance with a nano-indentation measurement. Using a priori knowledge concerning the density and elastic parameters of the coating and substrate, scanning the sample allows the obtaining of a coating thickness map in a fully remote, all-optical way, with an accuracy of about 4 microns for a coating of around 20-micron average thickness. Analyzing the transient thermal grating decay yields a thermal diffusivity value of <inline-formula> <math display="inline"> <semantics> <mrow> <mo stretchy="false">(</mo> <mn>5.0</mn> <mo>±</mo> <mn>1.6</mn> <mo stretchy="false">)</mo> </mrow> </semantics> </math> </inline-formula> × 10<inline-formula> <math display="inline"> <semantics> <msup> <mrow></mrow> <mrow> <mo>??</mo> <mn>6</mn> </mrow> </msup> </semantics> </math> </inline-formula> m<inline-formula> <math display="inline"> <semantics> <msup> <mrow></mrow> <mn>2</mn> </msup> </semantics> </math> </inline-formula>/s, in the range of low-carbon steels. The consistency of the results infers that the all-optical laser ultrasonics approach should be feasible for remote inspection of the quality of optically rough coated claddings in the harsh environment of a nuclear reactor.
29Applied Sciencesv.13(14), Jul 2023ArticleDesign and Application of the BiVib Audio-Tactile Piano Sample LibraryStefano Papetti / Federico Avanzini / Federico FontanaAppl. Sci. 2019, 9(5), 914;https://doi.org/10.3390/app9050914A library of piano samples composed of binaural recordings and keyboard vibrations has been built, with the aim of sharing accurate data that in recent years have successfully advanced the knowledge on several aspects about the musical keyboard and its multimodal feedback to the performer. All samples were recorded using calibrated measurement equipment on two Yamaha Disklavier pianos, one grand and one upright model. This paper documents the sample acquisition procedure, with related calibration data. Then, for sound and vibration analysis, it is shown how physical quantities such as sound intensity and vibration acceleration can be inferred from the recorded samples. Finally, the paper describes how the samples can be used to correctly reproduce binaural sound and keyboard vibrations. The library has potential to support experimental research about the psycho-physical, cognitive and experiential effects caused by the keyboard’s multimodal feedback in musicians and other users, or, outside the laboratory, to enable an immersive personal piano performance.

期刊基本信息表-B来源

编号期刊名称期刊简介国际标准期刊号(ISSN)一级分类二级分类学科领域引用得分影响因子公开出版费用收录-出版时限摘要和索引
0IFAC Journal of Systems and ControlIFAC Journal of Systems Control publishes significant, previously unpublished, and high-quality research papers containing generalizable, extensible and transferable innovations …2468-6018Physical Sciences and EngineeringComputer Science; EngineeringInformation Systems, Control and Systems Engineering2.81.9$3,3008days Acceptance to publicationMathSciNet;Scopus;INSPEC
1Annales d'UrologieLes Annales d'urologie évoluent!La revue "Annales d'urologie" s'est affirmée en 2004 avec une nouvelle formule née des traités Urologie et Techniques chirurgicales Urologie.Cette…0003-4401HealthMedicine
2Diabetes & MetabolismDiabetes Metabolism, publishes high-quality original research papers by internationally leading teams, forming a close link between hospital and research units. Official publicat…1262-3636Life SciencesBiochemistry, Genetics and Molecular BiologyMedicine and Dentistry (General), Endocrinology, Diabetes and Metabolism, Public Health and Health Policy127.2$4,1109days Time to first decision;14days Review time;7days Acceptance to publicationBIOSIS Biological Abstracts;Chemical Abstracts;Current Contents - Life Sciences;Embase;PubMed/Medline;Pascal Francis;Research Alert;Current Contents - Social & Behavioral Sciences;Science Citation Index
3EMC - Tratado de MedicinaEMC Medicina ha sido creado para los médicos de medicina general, aunque puede ser consultado por los especialistas que buscan una información fuera de su área de competencia. Cons…1636-5410HealthMedicineMedicine and Dentistry (General)
4Enfermería Intensiva (English Edition)Enfermería Intensiva (Intensive Care Nursing) is a peer reviewed journal that has been published for the past 27 years. It publishes original research articles on nursing intervent…2529-9840HealthNursingEmergency Rescue$2,040PubMed/Medline
5Current Opinion in PhysiologyWhat Current Opinions in Physiology publishes - COPHYS distinctly publishes invited short, annotated reviews in a Special Issue format. These short reviews are of recent (2-5 years…2468-8673Life SciencesBiochemistry, Genetics and Molecular BiologyPhysiology5.42.5$3,50018days Acceptance to publication
6European Journal of Trauma & DissociationThe European Journal of Trauma Dissociation / Revue Européenne du Trauma et de la Dissociation is the official journal of the European Society for Trauma and Dissociation and of …2468-7499Social Sciences and HumanitiesPsychologyCognitive Neuroscience, Psychology (General)1.62.1$1,6403days Acceptance to publicationScopus;Emerging Sources Citation Index (ESCI)
7Heart & LungHeart Lung: The Journal of Cardiopulmonary and Acute Care, the official publication of The American Association of Heart Failure Nurses, presents original, peer-reviewed articles…0147-9563HealthMedicine; NursingMedicine and Dentistry (General), Cardiology and Cardiovascular Medicine, Critical Care and Intensive Care Medicine, Pulmonary and Respiratory Medicine, Nursing4.22.8$3,31017days Time to first decision;36days Review time;11days Acceptance to publicationScopus;PubMed/Medline
8Journal of Financial IntermediationThe Journal of Financial Intermediation seeks to publish research in the broad areas of financial intermediation, investment banking, corporate finance, financial contracting, fina…1042-9573Social Sciences and HumanitiesEconomics, Econometrics and FinanceFinance, Economics and Econometrics, Strategy and Management7.15.2$3,84013days Acceptance to publicationRePEc;Gale Academic OneFile;Current Abstracts (EBSCO);Current Contents - Social & Behavioral Sciences;Gale Infotrac Custom;Social Sciences Citation Index;Journal of Economic Literature;ProQuest;Social Sciences Citation Index;Scopus;ABI/Inform
9Perioperative Care and Operating Room ManagementPublished four times per year, the objective of this online journal is to serve as a multidisciplinary, peer-reviewed source of information related to the administrative, economic,…2405-6030HealthMedicineSurgery, Management of Technology and Innovation, Anesthesiology and Pain Medicine, Nursing and Health Professions (General)0.9$2,56027days Time to first decision;56days Review time;6days Acceptance to publication
10European Journal of Operational ResearchThe European Journal of Operational Research (EJOR) publishes high quality, original papers that contribute to the methodology of operational research (OR) and to the practice of d…0377-2217Physical Sciences and Engineering; Social Sciences and HumanitiesMathematics; Decision SciencesApplied Mathematics, Modelling and Simulation, Information Systems and Management, Statistics, Probability and Uncertainty, Management Science and Operations Research, Computer Science (General)11.26.4$3,2909days Acceptance to publicationInternational Abstracts in Operations Research;Science Citation Index Expanded;Zentralblatt MATH;COMPENDEX;Current Contents - Engineering, Computing & Technology;CompuMath Citation Index;Research Alert;Mathematical Reviews;Executive Sciences Institute;Engineering Index;INSPEC;Management Contents;QCAS;Decision Sciences Web;Bio Systems;Academic Journal Guide (Chartered Association of Business Schools)
11European Journal of Obstetrics & Gynecology and Reproductive BiologyThe European Journal of Obstetrics Gynecology and Reproductive Biology has an open access companion journal European Journal of Obstetrics Gynecology and Reproductive Biology: …0301-2115Health; Social Sciences and HumanitiesMedicine; PsychologyObstetrics, Gynecology and Women's Health42.6$3,10049days Review time;5days Acceptance to publicationCurrent Contents - Clinical Medicine;PubMed/Medline;Embase;Pascal Francis;Science Citation Index Expanded;Elsevier BIOBASE;Scopus;Science Citation Index
12Journal of Controlled ReleaseThe Journal of Controlled Release (JCR) publishes high-quality research articles in the broad field of delivery science and technology. This includes drug delivery systems and all …0168-3659Life Sciences; Physical Sciences and EngineeringPharma; Chemical Engineering; Materials ScienceBiomaterials, Pharmaceutical Science17.110.8$4,54023days Time to first decision;39days Review time;11days Acceptance to publicationCAB International;Chemical Abstracts;Current Contents - Life Sciences;Embase;International Pharmaceutical Abstracts;Polymer Contents;Science Citation Index;PubMed/Medline;BIOSIS Citation Index
13Archives of Medical ResearchArchives of Medical Research publishes original peer-reviewed medical research in an attempt to bridge the gaps created by medical specialization. Contributions are grouped into th…0188-4409HealthMedicineMedicine and Dentistry (General)107.7$2,750Scopus;Embase;Current Contents - Life Sciences;Science Citation Index;PubMed/Medline;Embase;Chemical Abstracts
14Reumatología Clínica (English Edition)Reumatología Clínica is the official journal of two national rheumatology societies: the Spanish Sociedad Espa??ola de Reumatología (SER) and the Mexican Colegio Mexicano de Reumato…2173-5743HealthMedicineImmunology, Allergology and Rheumatology
15Microprocessors and MicrosystemsMicroprocessors and Microsystems: Embedded Hardware Design (MICPRO) is a journal covering all design and architectural aspects related to embedded systems hardware. This includes d…0141-9331Physical Sciences and EngineeringComputer Science; Energy; Materials ScienceHardware and Architecture, Electrical and Electronic Engineering, Software, Computer Networks and Communications, Control and Systems Engineering4.92.6$2,200173days Review time;16days Acceptance to publicationINSPEC;Research Alert;Cambridge Scientific Abstracts;Web of Science;Current Technology Index;ACM Computing Reviews;Biomedical Engineering Citation Index;Current Contents;Current Contents;Electronics and Communications Abstracts;Engineering Index;Science Citation Index;Computer Abstracts;Scopus
16Journal of Banking & FinanceThe Journal of Banking and Finance (JBF) publishes theoretical and empirical research papers spanning all the major research fields in finance and banking. The aim of the Journal o…0378-4266Social Sciences and HumanitiesEconomics, Econometrics and FinanceFinance, Economics and Econometrics5.73.7$3,1206days Acceptance to publicationUMI Data Courier;Journal of Money, Credit and Banking;Current Contents;Management Contents;ABI/Inform;Journal of Economic Literature;Social Sciences Citation Index;ABSCAN;RePEc;Scopus;Academic Journal Guide (Chartered Association of Business Schools);Scopus
17High Energy Density PhysicsHigh Energy Density Physics is an international journal covering original experimental and related theoretical work studying the physics of matter and radiation under extreme condi…1574-1818Physical Sciences and EngineeringPhysics and AstronomyAstronomy and Astrophysics, Atomic and Molecular Physics, and Optics, Nuclear and High Energy Physics3.21.6$2,5506days Acceptance to publicationScopus;Science Citation Index
18BurnsBurns aims to foster the exchange of information among all engaged in preventing and treating the effects of burns.The journal focuses on clinical, scientific and social aspects …0305-4179HealthMedicineSurgery, Critical Care and Intensive Care Medicine, Emergency Medicine4.32.7$3,19073days Review time;7days Acceptance to publicationPubMed/Medline;PubMed/Medline;Science Citation Index Expanded;Current Contents;Embase
19European Economic ReviewEstablished in 1969, European Economic Review is one of the oldest general-interest economics journals for all of Europe. It is intended as a primary publication for theoretical an…0014-2921Social Sciences and HumanitiesBusiness, Management and Accounting; Economics, Econometrics and Finance; Social SciencesFinance, Economics and Econometrics3.92.8$3,05041days Acceptance to publicationScopus;Current Contents;Social Sciences Citation Index;Journal of Economic Literature;ABI/Inform;UMI Data Courier;Journal of Planning Literature;RePEc
20Forensic Science International: GeneticsForensic Science International: Genetics is the premier journal in the field of Forensic Genetics. This branch of Forensic Science can be defined as the application of genetics to …1872-4973Health; Life Sciences; Physical Sciences and Engineering; Social Sciences and HumanitiesMedicine; Toxicology; Biochemistry, Genetics and Molecular Biology; Toxicology; Chemistry; Psychology; Social SciencesLaw, Forensic Medicine, Genetics7.93.1$4,6705days Acceptance to publicationScopus;Embase;PubMed/Medline;National Criminal Justice Reference Service
21Focus on Powder CoatingsFocus on Powder Coatings is a monthly newsletter providing concise, up-to-date summaries on all aspects of the powder coatings industry. It reports on business, market and technica…1364-5439Physical Sciences and EngineeringChemical Engineering; Materials ScienceAnalytical Chemistry, Materials Chemistry16days Acceptance to publication
22CarbonThe journal Carbon is an international multidisciplinary forum for communicating scientific advances in the field of carbon materials, including low-dimensional carbon-based nanost…0008-6223Physical Sciences and EngineeringChemistry; Materials ScienceBiomaterials, Materials Chemistry, Energy (General), Nanotechnology20.110.9$4,38016days Time to first decision;23days Review time;6days Acceptance to publicationElsevier BIOBASE;Chemical Abstracts;Current Contents;Engineering Index;Monthly & Author Index;Materials Science Citation Index;Pascal Francis;Scopus
23GeobiosGeobios publishes bimonthly in English original peer-reviewed articles of internationalinterest in any area of paleontology, paleobiology, paleoecology, paleobiogeography, (bio)s…0016-6995Physical Sciences and EngineeringEarth and Planetary SciencesPalaeontology, Stratigraphy3.61.6$2,620Pascal Francis;GeoSciTech;Science Citation Index;Geographical Abstracts;Biological Abstracts
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45Current BiologyCurrent Biology is a general journal that publishes original research across all areas of biology together with an extensive and varied set of editorial sections. A primary aim of …0960-9822Life SciencesAgricultural and Biological ScienceNeuroscience (General), Agricultural and Biological Sciences (General), Ecology, Evolution, Behavior and Systematics, Biochemistry, Genetics and Molecular Biology (General)
46Journal of Environmental ManagementThe Journal of Environmental Management is a peer reviewed journal for the publication of original research related to managing environmental systems and improving environmental qu…0301-4797Life Sciences; Physical Sciences and EngineeringEnvironmental Science; Environmental ScienceGeography, Planning and Development, Renewable Energy, Sustainability and the Environment, Management, Monitoring, Policy and Law, Pollution, Ecology, Waste Management and Disposal13.48.7$3,70024days Time to first decision;39days Review time;12days Acceptance to publicationScopus;Academic Journal Guide (Chartered Association of Business Schools);AGRICOLA;Biological Abstracts;BIOSIS Previews;Science Citation Index;Science Citation Index Expanded;Agriculture, Biological and Environmental Sciences;Periodical Abstracts PlusText;Engineering Village - GEOBASE;Pascal Francis;Embase;Environmental Sciences
47L'??volution PsychiatriqueA journal of reference for practitioners, researchers, and students in human sciencesFounded in 1925 and appearing every trimester starting in 1927, L’??volution Psychiatrique publ…0014-3855HealthMedicineClinical Psychology, Psychiatry and Mental Health0.60.6$2,440Social Sciences Citation Index;Current Contents - Social & Behavioral Sciences;Embase;Pascal Francis;IBZ;IBR;Psydoc-fr
48Seminars in Cancer BiologySeminars in Cancer Biology is a review journal dedicated to keeping scientists informed of developments in the field of molecular oncology on a topic by topic basis. Each issue is …1044-579XHealth; Life SciencesMedicine; Agricultural and Biological Science; Biochemistry, Genetics and Molecular BiologyBiochemistry, Cancer Research, Cell Biology, Molecular Biology2014.5$5,1507days Acceptance to publicationScopus;PubMed/Medline;Embase;Chemical Abstracts;Current Contents - Life Sciences
49Journal of Vascular NursingJournal of Vascular Nursing provides clinical information regarding aortic and peripheral aneurysms, upper and lower extremity arterial disease, acute and chronic venous disease, a…1062-0303HealthMedicine; NursingNursing1.71$2,750Scopus;PubMed/Medline;International Nursing Index

论文基本信息表-B来源

编号期刊名称期卷号日期标题作者DOI页码摘要
857Research in Veterinary ScienceVolume 92, Issue 220120401Sero-epidemiological study of the presence of hantaviruses in domestic dogs and cats from BelgiumA. Dobly, C. Cochez, E. Goossens, H. De Bosschere, ... P. Heymanhttps://doi.org/10.1016/j.rvsc.2011.02.003Pages 221-224Hantaviruses are worldwide rodent-borne pathogens infecting humans and other animals mainly through inhalation of aerosols contaminated with rodent excreta. Few data are available on hantavirus serology and geographical distribution in dogs and cats. We therefore screened sera from pet dogs (N = 410) and cats (N = 124) in two regions of Belgium, using IgG ELISA and IFA. We analysed the effect of the owner’s address as well as pet gender and age on hantavirus status. Hantavirus antibodies were found in both species with a significantly higher seroprevalence in cats than in dogs (16.9% vs. 4.9%, P = 0.001). More dogs were infected in highly forested southern Belgium (harbouring more rodents) than in northern Belgium (10.5% vs. 3.0%, P = 0.002). In the south, hantavirus sero-positive cats were found in more densely forested localities than sero-negatives ones were (P = 0.033). These results are consistent with the ecological variations of hantavirus risks in humans.
857Research in Veterinary ScienceVolume 92, Issue 220120401Implications and challenges of tuberculosis in wildlife ungulates in Portugal: A molecular epidemiology perspectiveMónica V. Cunha, Filipa Matos, Ana Canto, Teresa Albuquerque, ... Ana Botelhohttps://doi.org/10.1016/j.rvsc.2011.03.009Pages 225-235Mycobacterium bovis and, more rarely, Mycobacterium caprae, may cause zoonotic bovine tuberculosis (bTB) in an extensive range of animal species. In Portugal, during 2009, a remarkable raise of bTB incidence was registered in cattle along with an increase of new cases in wildlife. In this work, we reassess and update the molecular epidemiology of bTB in wild ungulates by including 83 new M. bovis and M. caprae isolates from wild boar and red deer obtained during 2008–2009. Spoligotyping identified 27 patterns in wild ungulates, including 11 patterns exclusive from deer and five from wild boar. The genetic relatedness of wildlife and livestock isolates is confirmed. However, the relative prevalence of the predominant genotypes is different between the two groups. Contrasting with the disease in livestock, which is widespread in the territory, the isolation of bTB in wildlife is, apparently, geographically localized and genotypic similarities of strains are observed at the Iberian level.
857Research in Veterinary ScienceVolume 92, Issue 220120401Evaluation of Spoligotyping and MIRU-VNTR for Mycobacterium bovis in Xinjiang, ChinaZhaogang Sun, Rui Cao, Miao Tian, Xuxia Zhang, ... Chuanyou Lihttps://doi.org/10.1016/j.rvsc.2011.03.012Pages 236-239Mycobacterium bovis, the causative agent of bovine tuberculosis (bTB) in cattle, is also a pathogen for human and other mammals. In this study, 406 cows were screened for bTB by both single intradermal comparative cervical tuberculin (SICCT) test and IFN-γ assay. 135 M. bovis were isolated from 31 SICCT and IFN-γ double-positive cows in Xinjiang Uygur Autonomous Region of China. Spoligotyping and MIRU-VNTR were evaluated for genotyping, and 4 and 7 genotypes were identified, respectively. A new combination of nine MIRU-VNTR loci was most discriminative for M. bovis clones from Xinjiang. Interestingly, two new spoligotypes (SB1903 and SB1904) and special repeat numbers of three loci (ETR-D, QUB 1895 and QUB 3336) were discovered in this study. These results indicated a specific epidemic conservation in Xinjiang, China. M. bovis strains with the unique genotypes were isolated from the herds maintaining parent cows imported from the bTB-free countries, suggesting a possible transmission from the local breed of Xinjiang brown cattle.
857Research in Veterinary ScienceVolume 92, Issue 220120401cDNA cloning and recombinant expression of a proliferation inducing ligand (APRIL) from Père David’s deerCui Min, Qi Wang, Yu-Qing Chen, Shuang-Quan Zhang, ... Yi-Jun Renhttps://doi.org/10.1016/j.rvsc.2011.01.022Pages 240-242In the present study, the cDNA of Père David’s deer APRIL (miAPRIL) was successfully amplified, and nucleotide sequence analysis showed that the open reading frame (ORF) of miAPRIL consists of 753 bases encoding 250-amino acids. Phylogenetic analysis exhibited high homology with the even-toed ungulates (artiodactyla). The extracellular soluble domain of the miAPRIL (misAPRIL) fragment was cloned into the expression vector pET43.1a and transformed into Escherichia coli BL21 (DE3) for recombinant expression. SDS–PAGE and western blotting analysis indicated that misAPRIL protein expression was successfully achieved. Indirect immunofluorescence staining demonstrated that misAPRIL has the ability to bind to the surface of Père David’s deer lymphocytes. Flow cytometry analysis revealed that misAPRIL was able to enhance lymphocytes survival in a dose-dependent manner.
857Research in Veterinary ScienceVolume 92, Issue 220120401Polymorphism in GHRH gene and its association with growth traits in Chinese native cattleBao Zhang, Gaofeng Zhao, Xianyong Lan, Chuzhao Lei, ... Hong Chenhttps://doi.org/10.1016/j.rvsc.2011.01.023Pages 243-246Growth hormone-releasing hormone (GHRH) is secreted by the hypothalamus and stimulates growth hormone (GH) released from the pituitary. Mutations detected in GHRH gene showed associations with animal production traits. The purpose of this study was to investigate the association of the GHRH gene with growth traits in Chinese native cattle. PCR-SSCP and sequencing were used to detect mutations of the GHRH gene in this study. One novel mutation 4251nt (C > T) was found and the frequencies of C allele were 0.8778 and 0.8476 for Qinchuan and Nanyang cattle, respectively. Body weight with the CT genotype was significantly higher (P < 0.05 or P < 0.01) than those with CC genotype for different growth periods (6, 12, 18, and 24 months old) in Nanyang cattle. Our findings suggested that polymorphism in bovine GHRH might be one of the important genetic factors to influence body weight.
857Research in Veterinary ScienceVolume 92, Issue 220120401Rumen fermentation and histology in light lambs as affected by forage supply and lactation lengthJ. ??lvarez-Rodríguez, E. Monleón, A. Sanz, J.J. Badiola, M. Joyhttps://doi.org/10.1016/j.rvsc.2011.03.010Pages 247-253This study determined whether the rumen fermentation and histology traits may reflect the feeding strategy in light lambs (22–24 kg). Thirty-two single Rasa Aragonesa lambs were assigned to one of four treatments in a 2 × 2 factorial design. The factors were the inclusion of forage in the diet (alfalfa grazing vs. concentrate-fed indoors) and lactation length (weaning at 13 kg vs. suckling until slaughter). A multivariate canonical analysis discriminated individuals among feeding strategies. The main function differentiated weaned concentrate-fed lambs from the rest according to dorsal sac papillae height, ventral sac muscular layer thickness and the proportion of rumen valerate. The second function differentiated suckling concentrate-fed lambs from the rest according to plasma urea levels. Lactation length played an important role on rumen histology and protein utilization, especially in concentrate-fed lambs. Alfalfa grazing light lambs had similar rumen morphometric measures and fermentation characteristics, regardless of milk access.
857Research in Veterinary ScienceVolume 92, Issue 220120401Toxocara vitulorum in suckling calves in the NetherlandsF.H.M. Borgsteede, M. Holzhauer, F.L. Herder, E.G. Veldhuis-Wolterbeek, C. Hegemanhttps://doi.org/10.1016/j.rvsc.2010.11.008Pages 254-256A Dutch farmer with beef cattle and suckling calves noticed one of the calves suffering from diarrhoea and observed large white worms near the perineum. In the faeces of this calf, eggs of Toxocara vitulorum were found. The majority of the cows in the herd were of the Piemontese breed previously imported from southern France. Treatment with albendazole was successful. Two other calves had positive faecal egg counts. One month after treatment of these calves with doramectin, no eggs were found. To prevent further infections on the farm and dissemination of this uncommon infection to other farms, the farmer decided to have all his cattle slaughtered.
857Research in Veterinary ScienceVolume 92, Issue 220120401Feline demodicosis caused by an unnamed speciesRui Kano, Ayako Hyuga, Jun Matsumoto, Sadao Nogami, ... Hiroshi Kamatahttps://doi.org/10.1016/j.rvsc.2011.03.015Pages 257-258A case of feline demodicosis is described in this report. A 13-year-old spayed female domestic short hair cat weighing 4.5 kg was being treated with cefovecin and alternately with prednisone or methylprednisolone. On further physical examination, the cat showed mild erythema and hair loss on the bridge of the nose, around the eyes, on the chin, on the side part of the breast and on the abdomen. A large number of Demodex mites were found in deep skin scrapings from the affected areas. The cat was then treated with ivermectin at 600 μg/kg administered SC daily. After 4 weeks of treatment, the cat was clinically normal with no mites detected in the skin scrapings from the face or breast areas. The mite responsible may represent a previously seen but as yet unnamed new species. This is third report that describes a case of feline demodicosis caused by a different, unnamed mite species that has different morphological characteristics to those of known Demodex mites and may represent a previously seen but as yet unnamed species.
857Research in Veterinary ScienceVolume 92, Issue 220120401Molecular cloning, expression and characterization of enolase from adult Haemonchus contortusKaikai Han, Lixin Xu, Ruofeng Yan, Xiaokai Song, Xiangrui Lihttps://doi.org/10.1016/j.rvsc.2011.03.008Pages 259-265Enolase represents a multifunctional protein involved in basic energy metabolism. In the present research, the enolase gene of Haemonchus contortus (HcENO) was cloned and characterized. Specific primers for the rapid amplification of cDNA ends (RACE) were designed based on the expression sequence tag (EST, GenBank Accession No. BF422728) to amplify the 3′- and 5′-ends of HcENO. The full length of cDNA from this gene was obtained by overlapping the sequences of 3′- and 5′-extremities and amplification by reverse transcription PCR. The biochemical activities of the recombinant protein HcENO, which was expressed in prokaryotic cells and purified by affinity chromatography, were analyzed by assays of enzymatic activity, stability to pH. The results showed that the cloned full length cDNA comprised 1583 bp and encoded a peptide with 434 amino acid residues which showed sequence similarity to several known enolases. The biochemical assay showed that the protein encoded by the HcENO exhibited enzymatic activity, whilst the HcENO was stable between pH 6 and 8. The natural enolase of H. contortus detected by immunoblot assay was approximately 49 kDa in size, and the recombinant HcENO was recognized strongly by serum from experimentally infected goats.
857Research in Veterinary ScienceVolume 92, Issue 220120401Perosomus elumbis, cerebral aplasia, and spina bifida in an aborted Thoroughbred foalI. Gerhauser, F. Geburek, P. Wohlseinhttps://doi.org/10.1016/j.rvsc.2010.11.009Pages 266-268Perosomus elumbis represents a rare congenital anomaly characterized by aplasia of the lumbosacral spinal cord and vertebrae. This anomaly is often associated with arthrogryposis and malformations of the urogenital and intestinal tract. This report describes the first case of perosomus elumbis in an aborted Thoroughbred foal associated with cerebral aplasia with meningocele, cranioschisis, spina bifida, a fused urogenital and intestinal tracts lined by a cutaneous mucosa without uterine glands, atresia ani, and arthrogryposis of the hind legs. Immunohistochemistry detected no abnormalities in the GFAP, S-100, vimentin, NeuN, doublecortin, and neurofilament expression pattern in the remnants of the brainstem. Pathogenic bacteria or equine herpesviruses one and four were not found using a microbiological investigation or polymerase chain reaction, respectively. The observed malformations may be induced by a severe developmental disturbance during the formation of the neural tube with secondary distortion of the surrounding mesoderm. Although the aetiology of this dysorganogenesis remains undetermined, inherited chromosomal mutations, an intake of different xenobiotics, and/or a disturbed metabolism of the mare may have caused the defect.
857Research in Veterinary ScienceVolume 92, Issue 220120401Matrix metalloproteinase expression in sheep with listerial meningoencephalitisFatma ??lhan, Yavuz Ulusoy, Mehmet Hal??gürhttps://doi.org/10.1016/j.rvsc.2011.01.019Pages 269-272Matrix metalloproteinases (MMPs) have been implicated in the pathogenesis of several central nervous system (CNS) diseases. In this study, we investigated the presence of Listeria monocytogenes antigens and detected the expression of MMP-9 and MMP-7 in the brains of 22 sheep with clinical signs and histopathological findings characteristic of listerial meningoencephalitis. Archived sections from the brainstem, cerebrum, and cerebellum were stained for immunohistochemistry. L. monocytogenes antigens were located mainly in the cytoplasm of neutrophils and some macrophages and/or extracellularly within microabscesses of the brainstem. MMP-9 was mainly immunolocalised in the endothelial cells, microglial cells, and neurons especially in inflammatory areas. MMP-7 immunoreactivity was detected in perivascular cuffs, microglial cells, and only a few neurons. Overall, immunohistochemistry in formalin-fixed, paraffin-embedded tissues is a useful tool for the diagnosis of encephalitic listeriosis caused by L. monocytogenes, and MMP-9 and MMP-7 may contribute to the pathogenesis of listerial meningoencephalitis.
857Research in Veterinary ScienceVolume 92, Issue 220120401Clinical and morphological investigations on the incidence of forms of rickets and their association with other pathological states in broiler chickensI. Dinevhttps://doi.org/10.1016/j.rvsc.2011.02.011Pages 273-277The purpose of investigations was to determine, by means of pathomorphological methods, the incidence of different rickets forms and their role for the occurrence of other illnesses in broiler chickens in Bulgaria.Clinical, blood biochemical, gross anatomy and histological investigations were carried out in broiler chickens with signs of rickets. The studies were performed in 12 broiler flocks in 4 farms located in different regions of the country. Based on macro- and microscopic lesions, alterations specific for hypocalcaemic rickets were observed in two farms, whereas signs of hypophosphataemic rickets – in the other two. The rickets diagnosis was confirmed by analysis of blood serum calcium, magnesium and inorganic phosphate concentrations.At the age of 30–35 days, various pathological states were observed in the same farms. The presented results suggested that existing problems in studied flocks were associated with an earlier occurrence of rickets.
857Research in Veterinary ScienceVolume 92, Issue 220120401The efficacy and safety of levobupivacaine administered by lumbosacral epidural route in conscious sheepRafael DeRossi, Amadeu B. Silva-Neto, Cassio T.D. Pompermeyer, Fabrício O. Frazílio, ... Andrea C.L. de Barroshttps://doi.org/10.1016/j.rvsc.2010.11.006Pages 278-282The aim of this study was to evaluate 3 doses of levobupivacaine (LB) epidurally administered in sheep. Six adult male 24–36 month-old sheep received levobupivacaine at 3 doses, LB05 (0.05 mg/kg), LB15 (0.15 mg/kg), and LB25 (0.25 mg/kg), and saline solution into the lumbosacral epidural space. Heart rate, arterial blood pressure (systolic, diastolic, and mean), respiratory rate, rectal, and skin temperature, local anesthesia, and ataxia were determined before treatment and at predetermined intervals. The duration of local anesthesia was 30 ± 5 min, 145 ± 27 min, and 290 ± 18 min for LB05, LB15, and LB25, respectively (P < 0.05). Ataxia determined for LB05, LB15, or LB25 was similar to the anesthetic times. There was an increase in heart rate and reduction in arterial pressure in LB25 (P < 0.05), whereas LB05 or LB15 did not affect these parameters. Lumbosacral epidural levobupivacaine is an appropriate choice for local anesthesia in sheep.
857Research in Veterinary ScienceVolume 92, Issue 220120401Cross-sectional study of hepatic CYP1A and CYP3A enzymes in hybrid striped bass, channel catfish and Nile tilapia following oxytetracycline treatmentN. Topic Popovic, T. Howell, J.G. Babish, P.R. Bowserhttps://doi.org/10.1016/j.rvsc.2011.03.003Pages 283-291Terramycin for Fish?? (oxytetracycline, OTC) is one of three approved drugs for therapeutic treatment of fish in the United States. Nothing is known, however, of the effects of this therapeutic on drug metabolizing enzymes in fish post-treatment. The main purpose of the study was to examine whether the fish CYP1A and CYP3A enzymes would cross-react with antibodies to known mammalian cytochrome P-450 forms (CYP1A1 and CYP3A). Observational feeding studies of OTC effects were conducted in hybrid striped bass, channel catfish and Nile tilapia. Oxytetracycline was mixed into the feed to achieve a daily dose of 82.8 mg per kg body weight at a feeding rate of 1% body weight per day. Hepatic microsomes of each fish were prepared and Western blotting of CYP1A1 and CYP3A4 and enzyme assays of CYP1A2 and CYP3A4 were performed prior to OTC treatment and on post-treatment days 1, 6, 11 and 21. Both goat anti-rat CYP1A1 and rabbit anti-human CYP3A4 showed good cross-reactivity with all three species in this study. All three species exhibited distinct perturbations in one or more of the variables examined on day 1 post-treatment. Immediately following the 10-day medication period, relative liver weight (RLW) of hybrid striped bass was increased 44% and remained elevated through post-treatment day 21. Increased CYP3A4 enzyme activity and protein abundance were noted in channel catfish and Nile tilapia, respectively. This observational approach demonstrated species differences both in control activities and in the timing and extent of hepatic responses to OTC. The unique perturbations of hepatic CYP450 enzymes in different fish species to OTC treatment observed in this study may have relevance for the use of additional antibiotics or other therapeutics used in aquaculture.
857Research in Veterinary ScienceVolume 92, Issue 220120401Biochemical properties of bull spermatozoa separated in iodixanol density solutionBlanka Beer-Ljubi??, Jasna Aladrovi??, Terezija Silvija Marenjak, Ivanka Maji??-Bali??, ... Suzana Milinkovi??-Turhttps://doi.org/10.1016/j.rvsc.2011.01.011Pages 292-294Bull spermatozoa samples contain variable portion of motile and normal morphology spermatozoa along with spermatozoa incapable of fertilization due to their pathologic changes. As semen quality is influenced by biochemical and morphological characteristics of all spermatozoa, the aim of the study was to separate spermatozoa in discontinuous iodixanol density gradient solution and to determine their cholesterol, phospholipid, triacylglycerol and lipid peroxide concentrations and creatine kinase activity. The study was performed in winter and included seven Simmental bulls aged 1.5–3.5 years. Semen samples were collected by use of artificial vagina. Upon evaluation of semen quality (volume, concentration and progressive sperm motility), the samples were centrifuged in iodixanol density solution to obtain two sperm fractions. The two fractions included sperms with progressive motility greater than 90% and less than 20%, respectively. A statistically significantly higher lipid peroxide concentration was determined in sperm fraction with <20% progressive motility. Different sperm subpopulations can be obtained by separating bull spermatozoa in different iodixanol density gradient solutions, while monitoring their biochemical properties can help assess the sperm quality.
857Research in Veterinary ScienceVolume 92, Issue 220120401Ultrasonography of the abomasum in 30 Saanen goatsU. Braun, D. Jacquathttps://doi.org/10.1016/j.rvsc.2011.03.013Pages 295-298This report describes the findings of ultrasonographic examination of the abomasum in 30 goats. A 5.0 MHz linear transducer was used to scan the left and right paramedian regions, the linea alba and the right abdominal wall of standing goats. The abomasum appeared as a heterogeneous, moderately echogenic structure with echogenic stippling. The abomasum could be seen from the linea alba in all the goats. The abomasal folds were easily identified in 19 goats and appeared as prominent echogenic bands. The cranial visible limit of the abomasum was ??0.3 2.34 cm from the xyphoid, and the caudal limit was 10.3 3.08 cm caudal to the xyphoid. The visible length of the abomasum was 10.7 1.96 cm and the maximum width 30.2 7.43 cm. The height of the abomasum was 6.7 1.79 cm.
857Research in Veterinary ScienceVolume 92, Issue 220120401Effect of hypoxia/reoxygenation on the contractility of the isolated bovine digital veinA. Risso, S. Comerma-Steffensen, J. Rojas, E. Ascanio, ... H. Zerpahttps://doi.org/10.1016/j.rvsc.2010.11.007Pages 299-305The bovine digital vasculature contractility has been implicated in the development of laminitis. To investigate the effect of hypoxia/reoxygenation on the contractility of isolated peripheral bovine digital veins (BDVs), vessel rings were studied under isometric conditions and submitted to 30 min of hypoxia (95%N2–5%CO2) and reoxygenation (95%O2–5%CO2) conditions, respectively. The BDVs contracted with a high K+ depolarizing solution, developed hypoxia-induced relaxation, followed by an increase in tension upon reoxygenation. In contrast, phenylephrine-contracted BDVs displayed a rapid, sustained and reversible hypoxia-induced contraction. Reoxygenation caused a rapid relaxation in phenylephrine-contracted BDVs. The presence of the endothelium did not modify the hypoxia/reoxygenation effects and hypoxia-induced contraction was still observed in a nominal Ca2+-free Krebs, however, the last effect was not maintained over time. The hypoxia-induced contraction in an isolated peripheral vein may contribute to the understanding of the physiology and pathophysiology of superficial venous smooth muscle contractility, particularly in the alteration of bovine digital haemodynamics under hypoxia/reoxygenation conditions.
857Research in Veterinary ScienceVolume 92, Issue 220120401Immunomodulation during and after castration under inhalation anaesthetic without genotoxic effects on equine lymphocytesA. Strasser, H. Kühnel, K. Velde, A. Dadakhttps://doi.org/10.1016/j.rvsc.2011.01.021Pages 306-310Genotoxic DNA damage due to inhalation anaesthesia has been demonstrated in human lymphocytes. In order to evaluate anaesthesia-associated changes in cell-mediated immunity on the basis of a potential DNA damage as a health risk in horses, single cell gel electrophoresis and lymphocyte proliferation assay were performed on equine lymphocytes which were obtained before, during and after regular castration under inhalation anaesthetic. No significant lymphocytic DNA damage due to isoflurane anaesthesia was observed, whereas lymphocyte proliferative reactivity and lymphocyte counts decreased significantly (p ?? 0.05) during and after anaesthesia. The present study thus indicates that the combined anaesthesia does not result in significant DNA damage, which hence cannot be held responsible for the observed changes in the immune response of equine lymphocytes. However, the recognized compromises of immune function ought to be considered especially in immunologically challenged animals.
857Research in Veterinary ScienceVolume 92, Issue 220120401Osteoclast progenitors from cats with and without tooth resorption respond differently to 1,25-dihydroxyvitamin D and interleukin-6H.E. Booij-Vrieling, T.J. de Vries, T. Schoenmaker, M.A. Tryfonidou, ... V. Evertshttps://doi.org/10.1016/j.rvsc.2011.03.014Pages 311-316Both vitamin D and inflammatory cytokines can stimulate osteoclast formation and activity. We studied the effect of 1,25-dihydroxycholecalciferol (1,25(OH)2D), and interleukin-6 (IL-6), on the formation and activity of feline osteoclasts, using peripheral blood mononuclear cells (PBMCs) from cats with and without tooth resorption (TR+ and TR??) as a source of osteoclast precursors. The formation of osteoclast-like cells (defined as multinucleated, tartrate-resistant acid phosphatase-positive cells) was assessed at 7 and 14 days. In the presence of M-CSF and RANKL, with and without IL-6, more osteoclasts were formed from TR?? PBMCs than from TR+ PBMCs on plastic. More osteoclasts were formed from TR+ PBMCs on bone slices in the presence of M-CSF/RANKL with 1,25(OH)2D. This opposite effect may be due to a higher expression of the vitamin D receptor (VDR) in TR+ osteoclasts and precursors on bone. Formation of resorption pits was analyzed and confirmed with scanning electron microscopy. In conclusion, we propose that TR+ PBMCs when cultured on bone are sensitive to 1,25(OH)2D, whereas the differentiation of TR?? PMBCs on bone seem more sensitive to IL-6, suggesting that osteoclast precursors from cats with and without tooth resorption respond differently to osteoclast stimulating factors.
857Research in Veterinary ScienceVolume 92, Issue 220120401Experimental evidence for competitive growth advantage of genotype VII over VI: Implications for foot-and-mouth disease virus serotype A genotype turnover in natureJ.K. Mohapatra, S. Subramaniam, N.K. Singh, A. Sanyal, B. Pattnaikhttps://doi.org/10.1016/j.rvsc.2011.01.030Pages 317-319In India, systematic genotype replacement has been observed for serotype A foot-and-mouth disease virus. After a decade of co-circulation of genotypes VI and VII, genotype VII emerged as the single dominant genotype since 2001. To derive possible explanations for such epochal evolution dynamics, in vitro intergenotype growth competition experiments involving both co- and superinfection regimes were conducted. Coinfection of BHK-21 cells demonstrated abrupt loss in the genotype VI viral load with commensurate increase in the load of genotype VII as measured by the genotype differentiating ELISA, RT-PCR and real-time RT-PCR. The superinfection dynamics was shaped by temporal spacing of infection, where the invading genotype VII took more number of passages than coinfection to eventually overtake the resident genotype VI. It was speculated that such superior replicative fitness of genotype VII could have been a possible factor for the ultimate dominance of genotype VII in nature.
857Research in Veterinary ScienceVolume 92, Issue 220120401Antiviral activity of Alpinia katsumadai extracts against rotavirusesHa-Hyun Kim, Hyung-Jun Kwon, Young Bae Ryu, Jong Sun Chang, ... Woo Song Leehttps://doi.org/10.1016/j.rvsc.2010.11.012Pages 320-323In vitro anti-rotavirus activity of Alpinia katsumadai (AK) extracts were evaluated against bovine G8P7 and porcine G5P7 rotaviruses in two different assay strategies, a mixed treatment assay and a post treatment assay. In the mixed treatment assay, six AK extracts AK-1 (EtOH extract), AK-3 (H2O layer), AK-5 (40% methanol fraction), and AK-9–11 (H2O extract, polysaccharide fraction, supernatant fraction) exhibited inhibitory activities against G5P7 rotavirus with the EC50 values ranging from 0.7 ± 0.4 to 33.7 ± 6.5 μg/mL. Extracts AK-1, AK-3, and AK-5 inhibited rotavirus infection against G8P7 rotavirus, the with EC50 values of 8.4 ± 2.2 μg/mL, 6.5 ± 0.8 μg/mL and 8.4 ± 5.0 μg/mL, respectively. By hemagglutination inhibition (HI) assay, six AK extracts completely inhibited viral adsorption onto human RBCs in both strains of rotaviruses at less than 11 μg/mL. However, in the post treatment assay, there was no anti activity shown against both strains of rotaviruses. As a result, six AK extracts were attributed mainly to having a strong interaction with hemagglutinin protein on the outer surface of rotavirus, resulting to blockage of viral adsorption.
857Research in Veterinary ScienceVolume 92, Issue 220120401First genetic characterization of equine adenovirus type 1 (EAdV-1) in TurkeyVeysel Soydal Ataseven, Tuba ??i??dem O??uzo??lu, Zeynep Ba??aran-Karap??nar, Seval Bilge-Da??alphttps://doi.org/10.1016/j.rvsc.2011.03.001Pages 324-326Equine adenovirus type 1 (EAdV-1) is a cause of repiratory tract infection in equids. In present study for the first time in Turkey, the prevalence of EAdV-1 in nasal swab samples obtained from horses showing respiratory symptoms was investigated by polymerase chain reaction (PCR), and molecular characterization of the hexon gene detected in the Turkish (TR) strain was performed. Overall, the prevalence of EAdV-1 was found low (1.4%) as indicated by a positive PCR reaction from the nasal swab extracts tested. Phylogenetic analysis based on the partial sequences of the hexon gene of a TR-EAdV-1 strain with those of previously isolated AdVs from different mammals and an EAdV-1 M1 strain showed that the EAdV-1 strains were placed into a unique cluster. Although the TR-EAdV-1 strain was closely related to CAV-1, CAV-2 and bat adenovirus reference strains, larger-scale studies are necessary to better understand the molecular epidemiology and population structure of EAdV-1 in Turkey.
857Research in Veterinary ScienceVolume 92, Issue 220120401Induction of feline immunodeficiency virus from a chronically infected feline T-lymphocyte cell lineTadafumi S. Tochikura, Yuko Naito, Yasunori Kozutsumi, Tsutomu Hohdatsuhttps://doi.org/10.1016/j.rvsc.2011.02.013Pages 327-332The infection of the feline T-lymphocyte cell line FeT-J with the feline immunodeficiency virus (FIV) Petaluma strain led to the establishment of nonvirus-producing cells. One clone (C15) obtained by limiting dilution was found to express FIV in response to chemical inducers of retroviruses. The chemical treatment of C15 cells led to not only FIV protein synthesis but also an augmentation of viral production. Examination of the C15 cell derivatives obtained by recloning revealed that 10–40% of treated cells constitutively expressed FIV antigens, whereas 100% with expressed FIV antigen in response to the inducer. Chemical induction resulted in more than a 100-fold increase in infectious viral production. The results suggest that a majority of FeT-J cells that are infected with FIV exist in a non-productive state. Establishing a cell line that can be non-productively infected by FIV may help determine the mechanisms of FIV latency.
857Research in Veterinary ScienceVolume 92, Issue 220120401Cowpox virus isolate virulent in humans shows attenuated phenotype in miceHartwig P. Huemer, Caroline Lassnig, Norbert Nowotnyhttps://doi.org/10.1016/j.rvsc.2011.03.011Pages 333-337We have cultured Cowpox virus (CPXV) from skin lesion material of a human patient from Austria. Phylogenetic comparison of the HA-gene revealed a rather homogeneous cluster with other local isolates from recent years, the A36R-gene was mostly related to elephant derived strains from Germany. Despite causing disease in human, the isolate AT/Carinthia/788/07 surprisingly even at high titers showed a highly reduced virulence in BALB/c mice upon intranasal inoculation as compared to vaccinia virus. This contrasts earlier reports on other CPXV isolates. Using shotgun DNA sequencing several insertions and deletions were found in genes presumably involved in host range, immune regulation as well as established virulence factors. These preliminary data could be an indication that CPXV strains with proven pathogenicity for humans may have reduced virulence in mice and vice versa. Additionally strains with a reduced virulence may have an advantage in persisting in less dense rodent populations.
857Research in Veterinary ScienceVolume 92, Issue 220120401Effects of hot-iron disbudding, using regional anaesthesia with and without carprofen, on cortisol and behaviour of calvesG. Stilwell, M.S. Lima, R.C. Carvalho, D.M. Broomhttps://doi.org/10.1016/j.rvsc.2011.02.005Pages 338-341The objective of this study was to assess cortisol and behaviour changes in calves hot-iron disbudded after different analgesic protocols. We assessed the response of 27 calves at 1, 3, 6 and 24 h after disbudding with regional anaesthesia (DA), regional anaesthesia plus carprofen (DAC), disbudded only (D) or sham-disbudded (ND). At 1 h cortisol was higher in D compared with all other groups. At 3 h DA showed higher cortisol than ND but did not differ from baseline. At 15 min and 1 h pain-related behaviours were more frequent in the D group than in all other groups. Group D (3 h) and DA (3 and 6 h) showed more ear flicks and head rubs compared with DAC and ND. We concluded that head rubbing, head shaking and ear flicking are useful behaviours for evaluating pain after hot-iron disbudding. Disbudding causes severe pain in calves and only the association of regional anaesthesia with carprofen assures good welfare for 24 h.
857Research in Veterinary ScienceVolume 92, Issue 220120401Lactic acid bacteria isolated from young calves – Characterization and potential as probioticsNatalia C. Maldonado, Clara Silva de Ruiz, María Claudia Otero, Fernando Sesma, María Elena Nader-Macíashttps://doi.org/10.1016/j.rvsc.2011.03.017Pages 342-349Lactic acid bacteria (LAB) are widely used as probiotics in humans and animals to restore the ecological balance of different mucosa. They help in the physiological functions of newborn calves that are susceptible to a variety of syndromes. The criteria for the selection of strains for the design of probiotic products are not available. Based in the host-specificity of the indigenous microbiota, 96 LAB isolates from faeces and oral cavity of calves were obtained. The surface properties were screened showing a small number of highly hydrophobic or autoagglutinating isolates. Also, a group produced H2O2 and were able to inhibit pathogens, and two strains were bacteriocin-producers. Some grew at very low pH and high bile concentrations.The strains sharing some of the specific properties evaluated were identified genetically, assayed their compatibility and exopolysaccharide production. The results allow going further in the establishment of criteria to select strains to be included in a multi-strain-probiotic-product to be further assayed in animals.
857Research in Veterinary ScienceVolume 92, Issue 120120201Editorial Boardhttps://doi.org/10.1016/S0034-5288(11)00476-0Page i
857Research in Veterinary ScienceVolume 92, Issue 120120201Endothelial hyperpolarizing factor increases acetylcholine-induced vasodilatation in pulmonary hypertensive broilers arterial ringsDiana I. Alvarez-Medina, Aureliano Hernandez, Camilo Orozcohttps://doi.org/10.1016/j.rvsc.2011.02.004Pages 1-6Pulmonary arterial hypertension (PAH) develops as result of imbalances between endothelium derived vasoconstrictors and vasodilators. Pulmonary hypertensive broiler chickens (PHBs) are deficient in NO production and endothelin-1 (ET-1) excess. With respect to prostacyclin, it appears that it does not alter vascular pulmonary tone in broilers. However, the role of Endothelium-Derived Hyperpolarizing Factor (EDHF) in PAH in broilers has not been determined. The possible involvement of EDHF in acetylcholine (Ach) induced vasodilatation was studied in pulmonary arterial rings taken from PHB and non-pulmonary hypertensive broilers (NPHBs). Ach induced higher vasodilatation in PHB than in NPHB. This dilatation seems to be directly related to the degree of PAH. Ach derived vasodilatation was inhibited, in PBH but not in NPHB, by blocking EDHF action with K+ or Apamin plus Charybdotoxin. It is proposed EDHF as an important vasodilator in the pulmonary arteries of PHB, which may play a compensatory role in PAH pathophysiology.
857Research in Veterinary ScienceVolume 92, Issue 120120201Computed tomography measurements of thoracic structures in 26 clinically normal goatsStefanie Ohlerth, Mareike Becker-Birck, Heinz Augsburger, Rahel Jud, ... Ueli Braunhttps://doi.org/10.1016/j.rvsc.2010.10.019Pages 7-12The present study was performed to provide computed tomographic (CT) reference values for structures in the thorax of 26 clinically normal Saanen goats. Animals were anesthetized, positioned in sternal recumbency and transverse images with a reconstructed 1.5 mm slice thickness were obtained by use of a 40-slice CT scanner. Absolute and relative measurements of the trachea, heart, cranial vena cava, thoracic aorta, caudal vena cava, right and left principal bronchus, right and left caudal lobar bronchus and the concomitant branch of the right and left pulmonary artery and vein, large caudal mediastinal lymph node and lung density were performed with dedicated software. Minimal to moderate interstitial or bronchopneumonia was incidentally found on CT in 24 animals. In conclusion, CT images obtained in this study can be used as a reference for the evaluation of thoracic diseases in goats.
857Research in Veterinary ScienceVolume 92, Issue 120120201California mastitis test scores as indicators of subclinical intra-mammary infections at the end of lactation in dairy cowsA.L. Bhutto, R.D. Murray, Z. Woldehiwethttps://doi.org/10.1016/j.rvsc.2010.10.006Pages 13-17Intramammary infections (IMI) during the dry period can be reduced through the use of dry cow therapy (DCT); in the future, its blanket use is likely to be questioned in the light of public concern regarding the routine use of antibiotics in food producing animals. One possible alternative is to limit DCT to cows with IMI just before drying off, which would require a quick, simple identification of sub-clinical IMI. In the present study we examined quarter milk samples obtained from 240 cows one week before and on the day of drying off, using the California mastitis test (CMT) and for IMI by bacteriological culture. The results indicated that high CMT scores at drying off may be good indicators of IMI: there was a significant association between the frequency of isolation of major pathogens and the CMT score in milk samples obtained one week before (Pearson’s χ2 = 27.04, df = 4, p < 0.001) and those at drying off (Pearson’s χ2 = 25.87, df = 4, p < 0.001).
857Research in Veterinary ScienceVolume 92, Issue 120120201Determination of flagellar types by PCR-RFLP analysis of enteropathogenic Escherichia coli (EPEC) and Shiga toxin-producing E. coli (STEC) strains isolated from animals in S??o Paulo, BrazilClaudia de Oliveira Ayala, Ana Carolina Ramos Moreno, Marina Baquerizo Martinez, Ylanna Kelner Burgos, ... Silvia Yumi Bandohttps://doi.org/10.1016/j.rvsc.2010.10.025Pages 18-23This study evaluated the polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) analysis of fliC for typing flagella antigen (H) of Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) strains isolated from different animals. The molecular typing of the H type was efficient in the determination of 93 (85%) strains. Two nonmotile (H-) E. coli strains showed a PCR-RFLP electrophoretic profile that did not match known H type patterns. The fliC nucleotide sequence of strains B2N and 4a revealed a nucleotide substitution at the restriction site and a nucleotide insertion that generated a stop codon, respectively. The results of this study showed that PCR-RFLP analysis of fliC is faster, less laborious and as efficient for the determination of H type E. coli isolated from animals, compared to serotyping and that it is useful in determining H type in nonmotile strains and strains expressing non-reactive H antigens. Moreover, the fliC sequence of strain B2N suggests that we could have found a new flagellin antigen type.
857Research in Veterinary ScienceVolume 92, Issue 120120201Tei index (myocardial performance index) and cardiac biomarkers in dogs with parvoviral enteritisMeric Kocaturk, Silvia Martinez, Oya Eralp, Asta Tvarijonaviciute, ... Zeki Yilmazhttps://doi.org/10.1016/j.rvsc.2010.10.018Pages 24-29Tei index (myocardial performance) and cardiac biomarkers were evaluated in dogs with parvoviral enteritis (PVE). Tei index was calculated as isovolumic contraction time plus isovolumic relaxation time divided by ejection time. Myocardial and skeletal muscle damages were assessed by serum levels of cardiac troponin I (cTnI), creatine (phospho) kinase, lactate dehydrogenase and aspartate aminotransferase. Serum magnesium level was also determined. According to treatment response, dogs were divided into the survivor (n = 20) and non-survivor groups (n = 23). Seven healthy dogs served as controls. The mean value of the Tei index was higher in non-survivors, compared with survivors (p < 0.02) and healthy controls (p < 0.01). Serum level of cTnI in non-survivors was higher than that of survivors and controls (p < 0.05). Tei index showed the highest sensitivity and specificity to predict mortality. The findings of an elevated Tei index and an increase in serum cTnI are factors associated with a poor prognosis in cases of canine parvovirosis.
857Research in Veterinary ScienceVolume 92, Issue 120120201Fatal bovine anaplasmosis in a herd with new genotypes of Anaplasma marginale, Anaplasma ovis and concurrent haemoplasmosisS. Hornok, A. Micsutka, I.G. Fernández de Mera, M.L. Meli, ... J. de la Fuentehttps://doi.org/10.1016/j.rvsc.2010.10.011Pages 30-35Haematological and molecular analysis of blood samples was carried out during an outbreak of bovine anaplasmosis in Hungary. Acute disease was observed in five animals, two of which died. Anaplasma-carrier state was diagnosed in 69 (92%) of cattle. Further evaluation of 24 blood samples revealed concurrent infections with Mycoplasma wenyonii and ‘Candidatus M. haemobos’ in 22 and 21 animals, respectively. In addition, two cows were identified with rickettsaemia. Regarding molecular investigation of potential hard tick vectors, Haemaphysalis inermis and Dermacentor marginatus males collected from the animals were PCR-negative. However, in one pool (out of 18) of Ixodes ricinus males, and in six pools (out of 18) of D. reticulatus males the msp4 gene of Anaplasma marginale was detected. In the same I. ricinus pool Anaplasma ovis was also identified. All ticks were negative for haemoplasmas. Anaplasma sequences yielded 97–99% homology to sequences deposited in the Genbank. This is the first report of fatal bovine anaplasmosis associated with divergent A. marginale genotypes and concurrent ‘Candidatus M. haemobos’ infection, as well as of an A. ovis strain in ticks collected from cattle.
857Research in Veterinary ScienceVolume 92, Issue 120120201Seroprevalence of canine herpesvirus-1 in Turkish dog populationKadir Ye??ilba??, Ebru Yal????n, Pelin Tuncer, Zeki Y??lmazhttps://doi.org/10.1016/j.rvsc.2010.10.016Pages 36-39Canine herpesvirus-1 (CHV-1) is the agent of reproductive and respiratory disorders in adult dogs, and the infection generally results in haemorrhagic disease conditions and neonatal death. In this study, virus neutralisation test that used complement (VNT) as well as in-house ELISA were utilised to investigate the CHV-1 seroprevalence in the Turkish dog population. Among the 560 serum samples, 39.3% of the samples tested by ELISA were CHV-1 positive while 29.4% of the samples tested by VNT were CHV-1 positive. Compared to the individual dogs (39.0%), there was a higher CHV-1 seroprevalence (62.1%) found in the colony dogs (62.1%) (p = 0.0002). However, there was an insignificant difference between male and female dogs. Although the highest antibody prevalence (56.7%) was found in Golden Retrievers, there were no significant variations detected among the dog breeds used in this study. Neutralizing antibody titres were very low (??1:16) in a high portion of the tested animals, confirming the rapid decrease of CHV-1 antibodies after the course of infection. The results of this study show that CHV-1 seroprevalence is moderately high in the Turkish dog population.
857Research in Veterinary ScienceVolume 92, Issue 120120201Effects of hyperimmune equine plasma on clinical and cellular responses in a low-dose endotoxaemia model in horsesG. Forbes, S. Church, C.J. Savage, S.R. Baileyhttps://doi.org/10.1016/j.rvsc.2010.10.020Pages 40-44Endotoxaemia is a major cause of equine morbidity, and plasma from horses immunised against Escherichia coli is used in its treatment. The aim of this study was to determine the effects of hyperimmune plasma on the clinical and leukocyte responses, including production and activity of TNFα, in an in vivo endotoxin challenge model. Pre-treatment with hyperimmune equine plasma had no significant effect on peak total plasma TNFα concentration (occurring 90 min after the administration of 30 ng/kg LPS). However, the bioavailable (unbound) TNFα measured by bioassay was significantly reduced in plasma-treated horses (1044.44 ± 193.93 pg/ml at 90 min) compared to saline treated controls (1373.92 ± 107.63 pg/ml; P = 0.05). Therefore, although pre-treatment with hyperimmune equine plasma did not significantly modify the clinical signs of endotoxaemia in this model, there was some evidence of reduced TNF bioactivity, which may be due to factors in the plasma which bind and reduce the activity of this cytokine.
857Research in Veterinary ScienceVolume 92, Issue 120120201Molecular identification and characterization of the intervening sequences (IVSs) within 23S ribosomal RNA (rRNA) genes of Taylorella asinigenitalis isolated in FranceAkihiro Tazumi, Sandrine Petry, Kyohei Hayashi, John E. Moore, ... Motoo Matsudahttps://doi.org/10.1016/j.rvsc.2010.10.004Pages 45-52In the helix 25 region, 32 French Taylorella asinigenitalis isolates carried at least one 23S rRNA gene not containing intervening sequences (IVSs). No IVSs in the region were identified in three isolates and the other remaining 29 isolates carried one or more IVSs (UCD-1TIVS1A, UCD-1TIVS1B and UK-1IVS1B) described already and two new kinds of IVS (TaIVS1C and TaIVS1D). In the helix 45 region, no T. asinigenitalis isolates not carrying any IVSs were identified. UK-1IVS2B was identified in the region from 26 isolates. Five new kinds of IVSs (TaIVS2D, E, F, G and H) occurred in the region in the 13 isolates. Distinctly different tandem repeat units (RS48 and RS32 and RS-A, -B and -C) were evident in both regions, respectively, from the French (n = 32) and American (n = 3) T. asinigenitalis isolates. Thus, several different kinds of tandem repeat units and their combinations in IVSs in both regions within the gene were shown in 32 French isolates.
857Research in Veterinary ScienceVolume 92, Issue 120120201Term placenta shows methylation independent down regulation of Cyp19 gene in animals with retained fetal membranesSandeep Ghai, Rachna Monga, T.K. Mohanty, M.S. Chauhan, Dheer Singhhttps://doi.org/10.1016/j.rvsc.2010.10.008Pages 53-59Retention of fetal membranes (RFM) is the major post-partum disorder in dairy cattle. Cyp19 gene encodes the aromatase enzyme responsible for catalyzing the rate limiting step in estrogen biosynthesis, an important hormone for placental maturation and expulsion. The present study was aimed for comparative analysis of Cyp19 gene expression and its epigenetic regulation in placental cotyledons of animals with and without RFM. Significantly lower expression of Cyp19 gene was found in placental samples of RFM affected animals in comparison to normal animals. Methylation analysis of 5 CpG dinucleotides of placenta specific Cyp19 gene promoter I.1 and proximal promoter, PII showed hypo-methylation of both PI.1 and PII in term placenta of normal and diseased animals. In conclusion, a mechanism other than promoter methylation is responsible for decreased aromatase expression in placental cotyledons of animals suffering from RFM.
857Research in Veterinary ScienceVolume 92, Issue 120120201Transcriptional profiling of antimicrobial peptides avian β-defensins in the chicken ovary during sexual maturation and in response to Salmonella enteritidis infectionG. Michailidis, M. Avdi, A. Argiriouhttps://doi.org/10.1016/j.rvsc.2010.10.010Pages 60-65Avian β-defensins (AvβDs) are antimicrobial peptides that play significant roles in the innate immune system in chickens. The aim of this study was to identify the types of AvβDs expressed in the chicken ovary, to investigate the effects of sexual maturation in the ovarian mRNA abundance and to determine the changes in their expression levels as a result to Salmonella enteritidis (SE) infection. RNA was extracted from the ovary of healthy prepubertal, sexually mature and aged birds, as well as from sexually mature and aged SE infected birds. Real-time PCR analysis revealed that 11 AvβDs genes were expressed in the chicken ovary. A significant up regulation of AvβD1, 3, 4, 5, 7 and 11 was observed in the ovary of sexually mature and aged birds. Furthermore, a significant up-regulation of AvβD4, 5, 7, 11 and 12 was observed in the ovary of SE infected sexually mature birds. These results suggest that the mRNA expression of at least six AvβDs increase with age in the ovary of laying hens, and that at least five AvβDs show an induction in their expression in response to SE infection, indicating an AvβD-mediated immune response mechanism in the chicken ovary.
857Research in Veterinary ScienceVolume 92, Issue 120120201Osteogenic proliferation and differentiation of canine bone marrow and adipose tissue derived mesenchymal stromal cells and the influence of hypoxiaDai-Jung Chung, Kei Hayashi, Chrisoula A. Toupadakis, Alice Wong, Clare E. Yellowleyhttps://doi.org/10.1016/j.rvsc.2010.10.012Pages 66-75The aim of this study was to compare the osteogenic and proliferative potential of canine mesenchymal stromal cells (cMSCs) derived from bone marrow (BM-cMSCs) and adipose tissue (AT-cMSCs). Proliferation potential was determined under varying oxygen tensions (1%, 5%, and 21% O2). Effects of reduced oxygen levels on the osteogenic differentiation of AT-cMSCs were also investigated. AT-cMSCs proliferated at a significantly faster rate than BM-cMSCs, although both cell types showed robust osteogenic differentiation. Culture in 5% and 1% O2 impaired proliferation in cMSC from both sources and osteogenic differentiation in AT-cMSCs. Our data suggests that AT-cMSCs might be more suitable for use in a clinical situation, where large cell numbers are required for bone repair, due to their rapid proliferation combined with robust osteogenic potential. Our data also suggests that the inhibitory effects of hypoxia on both cell proliferation and differentiation should be considered when using MSCs in a potentially hypoxic environment such as a fracture site.
857Research in Veterinary ScienceVolume 92, Issue 120120201Genetic variation of major histocompatibility complex BLB2 gene exon 2 in Hebei domestic chickenXiu-Li Guo, Hui-qin Zheng, Xiang-Long Li, Ying Li, ... Lan-Hui Lihttps://doi.org/10.1016/j.rvsc.2010.10.021Pages 76-79Genetic variation of MHC BLB2 gene exon 2 in Hebei domestic chicken was investigated, after PCR and sequencing of a 374 bp fragment (containing entire exon 2 (270 bp) of BLB2 gene) in 76 individuals. The results showed that along this fragment, there were 69 variable sites, of which 18 were novel variations, and 82 estimated haplotypes with the diversity of 0.960. In Hebei domestic chicken, the nucleotide diversity (π), the average number of nucleotide differences (k), the average number of nucleotide diversity of synonymous substitution (π(s)) and non-synonymous substitution (π(a)) in BLB2 gene exon 2 were 0.098, 24.688, 0.075, and 0.106, respectively; nine non-synonymous substitutions was exclusively found in the peptide-binding sites (PBS) region of BLB2 gene exon 2, inferring that these unique substitutions might be helpful to resist some special bacteria and pathogens. The higher genetic diversity of MHC BLB2 gene exon 2 in Hebei domestic chicken might be consistent with its more robust disease resistance.
857Research in Veterinary ScienceVolume 92, Issue 120120201Calreticulin expression in neoplastic versus normal dog mammary glands: A cDNA subtraction-based studyTakumi Okawa, Yuzo Kurio, Masahiro Morimoto, Toshiharu Hayashi, ... Takuya Mizunohttps://doi.org/10.1016/j.rvsc.2010.10.028Pages 80-91This is the first report describing the expression of canine calreticulin (cCRT) in canine mammary gland tumour (MGT). Using cDNA subtraction method, it is found that mRNAs of CRT, cathepsin A, ovostatin, and lactotransferrin were differentially expressed in mammary adenocarcinoma as compared to hyperplasia, both of which were obtained from the dog. Furthermore, the mRNA expression levels of CRT and cathepsin A were significantly higher in canine MGT samples than in nontumour samples. In contrast, immunohistochemical studies have indicated that the expression of cCRT protein found to be detected in most of mammary gland tissues and was not correlated to the types of canine MGTs. Furthermore, cCRT was molecularly cloned, and the amino acid sequence of cCRT was found to be very similar to those of other species. Further studies are required to elucidate additional roles of cCRT in canine MGT.
857Research in Veterinary ScienceVolume 92, Issue 120120201Isolation, propagation, identification and comparative pathogenicity of five Egyptian field strains of Eimeria tenella from broiler chickens in five different provinces in EgyptSomaia Saif Abu-Akkada, Ashraf Mahmoud Awadhttps://doi.org/10.1016/j.rvsc.2010.10.023Pages 92-95A battery trial was conducted to evaluate the comparative pathogenicity of five field strains of Eimeria tenella from Behera, Khafr El-Sheikh, Alexandria, Gharbia and Matrouh provinces in Egypt. Two-week-old broiler chickens were infected with 25 × 103 sporulated oocysts of each strain of E. tenella. The comparative pathogenicity of the strains was assessed by calculating body weight gain, feed conversion ratio, lesion scores, dropping nature scores, cecal scrapings, mortality percentage and oocysts count. Hematological parameters including hemoglobin (Hb) content, packed cell volume (PCV%) and total erythrocytic count, were also evaluated. There were different degrees of pathogenicity between the strains.
857Research in Veterinary ScienceVolume 92, Issue 120120201Detection of specific antibodies anti-Neospora caninum in the fallow deer (Dama dama)Justyna Bień, Bo??ena Moskwa, Marek Bogdaszewski, W??adyslaw Cabajhttps://doi.org/10.1016/j.rvsc.2010.10.014Pages 96-98Sera from 335 farmed fallow deer (Dama dama) at the breeding station in Kosewo Górne in the Mazurian Lake District, North-East Poland, were investigated for the presence of antibodies against Neospora caninum. The distribution of age groups was as follow: >4 years – 154 animals; 2 years – 76 animals; 1 year – 105 animals.Ten sera with the optical density exceeding 0.159 absorbance units (i.e., cut-off value) in ELISA test were also analyzed by Western blot. Western blot analysis revealed seroreactivity against immunodominant N. caninum antigens of 37, 25, and 16 kDa; however, in some sera additional bands were also visible.This is the first screening studies for antibodies against N. caninum in farmed fallow deer in Poland, in the region where neosporosis was confirmed in cattle and in farmed and free-ranging European red deer (Cervus elaphus).
857Research in Veterinary ScienceVolume 92, Issue 120120201Characterization of Bunostomum trigonocephalum and Bunostomum phlebotomum from sheep and cattle by internal transcribed spacers of nuclear ribosomal DNAC.R. Wang, J.F. Gao, X.Q. Zhu, Q. Zhaohttps://doi.org/10.1016/j.rvsc.2010.10.024Pages 99-102In the present study, samples representing Bunostomum trigonocephalum and Bunostomum phlebotomum from sheep and cattle in Heilongjiang Province, China, were characterized and grouped genetically by the first (ITS-1) and second (ITS-2) internal transcribed spacers (ITS) of nuclear ribosomal DNA (rDNA). The rDNA region including the ITS-1, 5.8S, ITS-2, and flanking 18S and 28S rDNA sequences was amplified by polymerase chain reaction (PCR), then sequenced and compared with that of other members of the hookworms available in GenBank??, and phylogenetic relationships between them were reconstructed using the Maximum-Parsimony method. The ITS-1, 5.8S, and ITS-2 sequences of the sheep hookworm were 381, 153, and 231 bp in length, respectively, and the corresponding sequences of the cattle hookworm were 392, 153, and 240 bp in length. The identity of ITS sequences of B. trigonocephalum and B. phlebotomum from sheep and cattle was 87.4%. A PCR-linked restriction fragment length polymorphism (PCR-RFLP) assay using restriction endonuclease Nde I was established for the unequivocal differentiation of the two hookworm species. Phylogenetic analyses based on the ITS sequences revealed that B. trigonocephalum and B. phlebotomum were closely related, but they represent two different species.
857Research in Veterinary ScienceVolume 92, Issue 1201202012-Aminoethoxydiphenyl borate (2-APB) reduces respiratory burst, MMP-9 release and CD11b expression, and increases l-selectin shedding in bovine neutrophilsI. Conejeros, E. Jara, M.D. Carretta, P. Alarcón, ... R.A. Burgoshttps://doi.org/10.1016/j.rvsc.2010.10.005Pages 103-110This study describes the effect of 2-aminoethoxydiphenyl borate (2-APB), a putative store-operated calcium (Ca2+) entry (SOCE) inhibitor, on reactive oxygen species (ROS) production, matrix metalloproteinase 9 (MMP-9) release, CD11b and l-selectin (CD62L) expression, size changes and apoptosis in bovine neutrophils stimulated with platelet-activating factor (PAF). It was observed that doses ??1 μM 2-APB significantly reduced ROS production, whereas 50 and 100 μM 2-APB reduced MMP-9 release induced by PAF. Moreover, concentrations ??10 μM 2-APB reduced CD11b expression and increased l-selectin shedding. PAF induced size changes in neutrophils, and this effect was inhibited by 2-APB. From this work it is possible to conclude that 2-APB at concentrations that inhibit SOCE responses was able to inhibit ROS and MMP-9 release and CD11b expression, and increase l-selectin shedding, suggesting that the Ca2+ channel involved in SOCE is a potential target for the development of new anti-inflammatory drugs in cattle.
857Research in Veterinary ScienceVolume 92, Issue 120120201Pharmacokinetics in pulmonary epithelial lining fluid and plasma of ampicillin and pivampicillin administered to horsesLotte Winther, Keith Edward Baptiste, Christian Friishttps://doi.org/10.1016/j.rvsc.2010.11.001Pages 111-115Ampicillin concentrations in pulmonary epithelial lining fluid (PELF) and plasma was studied after single intravenous ampicillin administration (15 mg/kg) or single intragastric administration of its prodrug, pivampicillin (19.9 mg/kg) to horses and discussed in relation to minimum inhibitory concentrations (MIC) of common equine respiratory pathogens.After intravenous administration, elimination of ampicillin was fast and not detectable in plasma after 12 h in three out of six horses. Pivampicillin was absorbed well in non-fasted horses with an oral bioavailability of 36%. The degree of penetration of ampicillin into PELF, as described by the AUCPELF/AUCplasma ratio from 0 to 12 h was 0.40 after intravenous administration and 1.00 after pivampicillin administration.In horses, ampicillin administered either intravenously or orally, in the form of pivampicillin, can provide clinically relevant drug concentrations in PELF for at least 12 h, when treating susceptible equine respiratory pathogens (e.g. streptococci). Treatment of other bacterial pathogens requires susceptibility testing and possibly more frequent dosing, depending of minimum inhibitory concentrations (MIC) values.
857Research in Veterinary ScienceVolume 92, Issue 120120201Effects induced by exercise on lymphocyte β-adrenergic receptors and plasma catecholamine levels in performance horsesB. Cuniberti, P. Badino, R. Odore, C. Girardi, G. Rehttps://doi.org/10.1016/j.rvsc.2010.11.002Pages 116-120The effect of dynamic exercise on complete blood cell count, lymphocyte β-adrenergic receptor and plasma catecholamine (adrenaline and noradrenaline) levels in horses performing different disciplines were investigated during rest and after exercise.Blood samples were collected from jumping horses (n = 6), Arabian Endurance horses (n = 6) and Standardbred trotters (n = 6) before and immediately after competition. Dynamic exercise caused a significant increase in red blood cell count (Standardbred trotters: P = 0.0012), haemoglobin concentration (jumping horses: P = 0.001; Standardbred trotters: P = 0.01), haematocrit percentage (Standardbred trotters: P = 0.005), neutrophil percentage (jumping horses: P = 0.0003), lymphocyte percentage (jumping horses: P = 0.0003), monocyte percentage (Standardbred trotters: P = 0.0008), lymphocyte β-AR numbers (jumping horses: P = 0.01; Arabian Endurance horses: P = 0.016; Standardbred trotters: P = 0.05), plasma adrenaline concentration (Standardbred trotters: P = 0.0001) and plasma noradrenaline levels (Standardbred trotters: P = 0.003). It is concluded that acute increases in plasma catecholamine concentrations depended on the exercise performed and may induce up-regulation of β-AR in equine lymphocytes. However, the exact mechanism of β-AR up-regulation still remains unclear.
857Research in Veterinary ScienceVolume 92, Issue 120120201Factors affecting rectal temperature measurement using commonly available digital thermometersJonathan M. Naylor, Renee M. Streeter, Paul Torgersonhttps://doi.org/10.1016/j.rvsc.2010.10.027Pages 121-123Rectal temperature measurement is an essential part of physical examination of cattle and some physiological experiments. Modern digital thermometers are often used to measure rectal temperatures by students; this study describes their reliability and appropriate use. Students measured rectal temperature on 53 occasions using their personal digital thermometer and techniques gained from previous instruction, rectal temperature was also measured by an experienced person using a Cornell mercury thermometer completely inserted in the rectum. Cornell mercury thermometers values were 38.95 ± 0.05 °C (mean ± 1 SE, n = 53). Student rectal temperature measurements using their initial technique were nearly 0.5 °C lower, 38.46 ± 0.07 °C. After receiving instruction to insert the digital thermometer to the window, student obtained values were 38.77 ± 0.06 °C; these are significantly higher than with the student’s initial technique and closer to those obtained with a Cornell thermometer. In a series of 53 water bath tests, student owned thermometers recorded similar mean values to those of a traceable (reference) digital thermometer, Cornell mercury thermometer readings were 0.2 °C higher. 10 individual digital thermometers were repeatedly tested against a traceable thermometer in a water bath, one was inaccurate. In a separate experiment a trained clinician tested the effect of angle of insertion of a digital thermometer on temperature readings and the affect was <0.1 °C. We conclude that accurate temperature measurements using digital thermometers are only likely if the thermometer is inserted to the beginning of the window and the thermometer’s accuracy is checked periodically.
857Research in Veterinary ScienceVolume 92, Issue 120120201Effect of melatonin on DNA damage of bovine cumulus cells during in vitro maturation (IVM) and on in vitro embryo developmentL. Takada, A. Martins Junior, G.Z. Mingoti, J.C.C. Balieiro, ... L.A. Coelhohttps://doi.org/10.1016/j.rvsc.2010.11.004Pages 124-127The effect of melatonin during in vitro maturation (IVM) on DNA damage of cumulus cells (CCs) from bovine cumulus–oocyte complexes (COCs) and embryo development was evaluated. COCs from abattoir ovaries were cultured in maturation medium (MM) with 0.5 μg/ml FSH and 5.0 μg/ml LH (FSH–LH); 10??9 M melatonin (MEL) or FSH–LH + MEL (FSH–LH–MEL). After 24 h of in vitro maturation, the CCs surrounding the oocyte were subjected to DNA analysis by Comet assay. After in vitro fertilization and in vitro embryo culture, the embryo development rates were evaluated on day 2 post insemination (cleavage) and days 7–8 (blastocyst). The percentage of CCs with no DNA damage was significantly superior in MEL group (37.6 ± 2.4) than in FSH–LH–MEL (28.0 ± 2.4) and FSH–LH (17.8 ± 2.41) groups. Cleavage and blastocysts rates were similar among groups. Melatonin during IVM protects the CCs from DNA damage but this effect did not influence embryo development in vitro.
857Research in Veterinary ScienceVolume 92, Issue 120120201Haematological values of post-laying Arrau turtle (Podocnemis expansa) in the Orinoco River, VenezuelaM. Rossini, P.A. Blanco, E. Marín, S. Comerma-Steffensen, H. Zerpahttps://doi.org/10.1016/j.rvsc.2010.10.026Pages 128-131The Arrau turtle (Podocnemis expansa) is an endangered species, as a result of long-lasting, unsustainable exploitation. To obtain reference haematological values from the wild Podocnemis expansa during post-laying, 20 turtles were captured in the Orinoco River. Blood was obtained from the dorsal cervical sinus in lithium heparin tubes. Red blood cells (RBC), white blood cells (WBC), thrombocytes (TC), packed cell volume (PCV), plasmatic protein (PP), haemoglobin (Hgb), mean corpuscular volume (MCV) and differential leukocyte count were determined. Haematological values were: RBC 0.9 × 109/L, WBC 5.7 × 109/L, TC 5.4 × 109/L, PCV 35.6%, PP 4.2 g/dL, Hgb 11.8 g/dL, MCV 411 fL. The differential leukocyte count comprised: 71% heterophils, 23% lymphocytes, 3% eosinophils, 1.6% basophils, and 1% monocytes. The reports of reference haematology values for the wild P. expansa are limited; therefore, the results presented herein contrast with those values obtained in captivity. This study represents a contribution to the referential haematological values of the wild P. expansa.

期刊基本信息表-C来源

编号期刊名称期刊链接国际标准期刊号（ISSN）期刊简介影响因子近5年影响因子
1Adaptationhttps://academic.oup.com/adaptation1755-0645Adaptation is the leading international, peer-reviewed journal of adaptation studies. The journal actively contributes to the development and visibility of adaptation studies as a field of academic enquiry and seeks to advance methodological approaches to the process.Find out more about subscriptions to AdaptationFind out more about the journal
2Aesthetic Surgery Journalhttps://academic.oup.com/asj1527-330X3.15.2 Surgery 50 out of 213
3Aesthetic Surgery Journal Open Forumhttps://academic.oup.com/asjopenforum2631-4797
4African Affairshttps://academic.oup.com/afraf1468-2621African Affairs is published on behalf of the Royal African Society and is the top ranked journal in African Studies. It is an inter-disciplinary journal, with a focus on the politics and international relations of sub-Saharan Africa …Find out more2.83.3
5Age and Ageinghttps://academic.oup.com/ageing1468-28346.79
6BioSciencehttps://academic.oup.com/bioscience/1525-3244
7Alcohol and Alcoholismhttps://academic.oup.com/alcalc1464-35022.82.8
8American Entomologisthttps://academic.oup.com/ae2155-9902
9The American Historical Reviewhttps://academic.oup.com/ahr1937-5239The American Historical Review (AHR) is the official publication of the American Historical Association (AHA). The AHR has been the journal of record for the historical profession in the United States since 1895 …Find out more1.52.0 History 7 out of 101
10American Journal of Clinical Pathologyhttps://academic.oup.com/ajcp1943-7722
11American Journal of Comparative Lawhttps://academic.oup.com/ajcl2326-9197Devoted to comparative and transnational legal studies including comparative law, transnational legal history and theory, private international law, conflict of laws, and the study of international legal systems …Find out more1.51.3
12American Journal of Epidemiologyhttps://academic.oup.com/aje1476-625655.8
13American Journal of Health-System Pharmacyhttps://academic.oup.com/ajhp1535-2900
14American Journal of Hypertensionhttps://academic.oup.com/ajh1941-72253.23
15The American Journal of Jurisprudencehttps://academic.oup.com/ajj2049-6494The American Journal of Jurisprudence is an international journal publishing critical discussions of the moral foundations of law and legal systems, exploring current and historical issues in ethics, philosophy of law or …Find out more
16American Journal of Legal Historyhttps://academic.oup.com/ajlh2161-797XThe American Journal of Legal History was founded in 1957 and was the first English-language periodical in the field. The journal is now published by Oxford University Press …Find out more
17American Law and Economics Reviewhttps://academic.oup.com/aler1465-7260The Review is a refereed journal, published twice a year. It maintains the highest scholarly standards, and at the same time endeavours to publish international work that is accessible to the full range of membership in the ALEA …Find out more1.51.3
18American Literary Historyhttps://academic.oup.com/alh1468-4365Covering the study of US literature from its origins through the present, American Literary History provides a much-needed forum for the various, often competing voices of contemporary literary inquiry …Find out more
19Analysishttps://academic.oup.com/analysis1467-8284Analysis is the most established and esteemed forum in which to publish short discussions of topics in philosophy …Find out more
20Animal Frontiershttps://academic.oup.com/af2160-6064
21Annals of Behavioral Medicinehttps://academic.oup.com/abm1532-4796
22Annals of Botanyhttps://academic.oup.com/aob1095-8290
23Annals of the Entomological Society of Americahttps://academic.oup.com/aesa1938-2901
24Annals of Work Exposures and Healthhttps://academic.oup.com/annhyg2398-7316
25Antibody Therapeuticshttps://academic.oup.com/abt2516-4236
26AoB PLANTShttps://academic.oup.com/aobpla2041-2851
27Applied Economic Perspectives and Policyhttps://academic.oup.com/aepp2040-5804
28Applied Linguisticshttps://academic.oup.com/applij1477-450XApplied Linguistics publishes research into language with relevance to real-world issues. The journal is keen to help make connections between scholarly discourses, theories, and research methods…Find out more3.65.6 Linguistics 18 out of 127
29Arbitration Internationalhttps://academic.oup.com/arbitration1875-8398Launched in 1985, Arbitration International provides quarterly coverage for national and international developments in the world of arbitration …Find out more
30Arbitration Law Reports and Reviewhttps://academic.oup.com/alrr2044-9887Important notice to authors:Arbitration Law Reports and Review has ceased publication.Find out more
31Archives of Clinical Neuropsychologyhttps://academic.oup.com/acn1873-58432.63
32Aristotelian Society Supplementary Volumehttps://academic.oup.com/aristoteliansupp1467-8349The Aristotelian Society Supplementary Volume is a commission-only journal and is published annually in June. It contains the Symposia to be read at the Annual Joint Session of the Aristotelian Society and the Mind Association …Find out more
33Arthropod Management Testshttps://academic.oup.com/amt2155-9856
34Astronomy & Geophysicshttps://academic.oup.com/astrogeo1468-4004Astronomy & Geophysics publishes short review papers on subjects within the remit of the Royal Astronomical Society: astronomy, astrophysics, cosmology, planetary science, solar-terrestrial physics, global and regional geophysics and the history of these topics.Find out more0.80.7
35Behavioral Ecologyhttps://academic.oup.com/beheco1465-7279
36Bioinformaticshttps://academic.oup.com/bioinformatics1367-4811
37Bioinformatics?Advanceshttps://academic.oup.com/bioinformaticsadvances2635-0041Bioinformatics Advances is a fully open access, peer-reviewed journal published jointly by Oxford University Press and by the International Society for Computational Biology.Find out more
38Biological Journal of the Linnean Societyhttps://academic.oup.com/biolinnean1095-8312The Biological Journal of the Linnean Society is a direct descendant of the oldest biological journal in the world, which published the epoch-making papers on evolution by Darwin and Wallace… Find out more1.92.0 Evolutionary Biology 39 out of 52
39Biology Methods and Protocolshttps://academic.oup.com/biomethods2396-8923
40Biology of Reproductionhttps://academic.oup.com/biolreprod1529-72683.64.2
41Biometrikahttps://academic.oup.com/biomet1464-35102.73.2
42BioSciencehttps://academic.oup.com/bioscience1525-3244
43Bioscience, Biotechnology, and Biochemistryhttps://academic.oup.com/bbb1347-6947
44Biostatisticshttps://academic.oup.com/biostatistics1468-4357Biostatistics is an online only journal publishing papers that develop innovative statistical methods with applications to the understanding of human health and disease, including basic biomedical sciences …Find out more2.13.8
45BJS Openhttps://academic.oup.com/bjsopen2474-9842
46Botanical Journal of the Linnean Societyhttps://academic.oup.com/botlinnean1095-8339The Botanical Journal of the Linnean Society publishes original papers on systematic and evolutionary botany and comparative studies of both living and fossil plants...Find out more2.42.5 Plant Sciences 106 out of 238
47Brainhttps://academic.oup.com/brain1460-215614.514.6
48Brain Communicationshttps://academic.oup.com/braincomms2632-1297
49Brief Treatment and Crisis Interventionhttp://academic.oup.com/btcint1474-3329
50Briefings in Bioinformaticshttps://academic.oup.com/bib1477-4054

论文基本信息表-C来源

期刊名称年份期卷号标题DOI引用信息作者出版日期作者单位页码摘要
Journal of Radiation Research2001Volume 42, Issue 4, DecemberEnvironmental Kinetics of Radioactive Nuclideshttps://doi.org/10.1269/jrr.42.436Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 436–438,2001/12/1"436-438"
Journal of Radiation Research2001Volume 42, Issue 4, DecemberEnvironmental and Ecological Effects of Environmental Pollutantshttps://doi.org/10.1269/jrr.42.439Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 439–441,2001/12/1"439-441"
Journal of Radiation Research2001Volume 42, Issue 4, Decemberp53 Function and Its Regulation Relevant to Adaptive Response to Radiationhttps://doi.org/10.1269/jrr.42.442Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 442–443,2001/12/1"442-443"
Journal of Radiation Research2001Volume 42, Issue 4, DecemberNuclear Fuel Cycle for 21 Century and Public Radioprotectionhttps://doi.org/10.1269/jrr.42.444Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 444–445,2001/12/1"444-445"
Journal of Radiation Research2001Volume 42, Issue 4, DecemberFront-runners in Study of Apoptosis: Mechanism and Significance in the Studies on Radiobiological Effectshttps://doi.org/10.1269/jrr.42.446Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 446–447,2001/12/1"446-447"
Journal of Radiation Research2001Volume 42, Issue 4, DecemberMicrobeam Irradiation: Studies on the Mechanism of Injuries to Cells and Trials for the Elucidation of Radiation-induced Adaptive Responsehttps://doi.org/10.1269/jrr.42.448Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 448–451,2001/12/1"448-451"
Journal of Radiation Research2001Volume 42, Issue 4, DecemberRadiation-induced Biological Response against Low Dose and Low Dose Rate of Irradiationshttps://doi.org/10.1269/jrr.42.452Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 452–454,2001/12/1"452-454"
Journal of Radiation Research2001Volume 42, Issue 4, DecemberRadiation-induced Oxidative Injuries—Biological Significance and Mechanism of Protectionhttps://doi.org/10.1269/jrr.42.455Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 455–456,2001/12/1"455-456"
Journal of Radiation Research2001Volume 42, Issue 4, DecemberNew DNA Polymeraseshttps://doi.org/10.1269/jrr.42.457Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 457–458,2001/12/1"457-458"
Journal of Radiation Research2001Volume 42, Issue 4, DecemberRadiation-induced Genomic Instabilityhttps://doi.org/10.1269/jrr.42.459Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 459–461,2001/12/1"459-461"
Journal of Radiation Research2001Volume 42, Issue 4, DecemberDNA Double-Strand Breaks-Repair and Transmission of Signalshttps://doi.org/10.1269/jrr.42.462Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 462–463,2001/12/1"462-463"
Journal of Radiation Research2001Volume 42, Issue 4, DecemberJRRS and JSRC Joint Workshop: Research Collaboration System between Radiobiology and Radiochemistryhttps://doi.org/10.1269/jrr.42.464Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 464–466,2001/12/1"464-466"
Journal of Radiation Research2001Volume 42, Issue 4, DecemberRadiation Epidemiology and Radiation Exposurehttps://doi.org/10.1269/jrr.42.467Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 467–473,2001/12/1"467-473"
Journal of Radiation Research2001Volume 42, Issue 4, DecemberEnvironmental Effects of Radiationshttps://doi.org/10.1269/jrr.42.474Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 474–478,2001/12/1"474-478"
Journal of Radiation Research2001Volume 42, Issue 4, DecemberRadiation Damage and Repair (I)https://doi.org/10.1269/jrr.42.479Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 479–487,2001/12/1"479-487"
Journal of Radiation Research2001Volume 42, Issue 4, DecemberRadiation Damage and Repair (II)https://doi.org/10.1269/jrr.42.488Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 488–497,2001/12/1"488-497"
Journal of Radiation Research2001Volume 42, Issue 4, DecemberAdaptive Response to Radiation (I)https://doi.org/10.1269/jrr.42.498Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 498–506,2001/12/1"498-506"
Journal of Radiation Research2001Volume 42, Issue 4, DecemberAdaptive Response to Radiation (II)https://doi.org/10.1269/jrr.42.507Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 507–514,2001/12/1"507-514"
Journal of Radiation Research2001Volume 42, Issue 4, DecemberEffects of Radiation (I)https://doi.org/10.1269/jrr.42.515Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 515–523,2001/12/1"515-523"
Journal of Radiation Research2001Volume 42, Issue 4, DecemberEffects of Radiation (II)https://doi.org/10.1269/jrr.42.524Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 524–533,2001/12/1"524-533"
Journal of Radiation Research2001Volume 42, Issue 4, DecemberTherapeutic Radiobiologyhttps://doi.org/10.1269/jrr.42.534Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 534–542,2001/12/1"534-542"
Journal of Radiation Research2001Volume 42, Issue 4, DecemberNon-ionizing Radiations and Radiation Physicshttps://doi.org/10.1269/jrr.42.543Journal of Radiation Research, Volume 42, Issue 4, December 2001, Pages 543–545,2001/12/1"543-545"
Journal of Radiation Research2002Volume 43, Issue 1, MarchPre-irradiation at a Low Dose-rate Blunted p53 Responsehttps://doi.org/10.1269/jrr.43.1Journal of Radiation Research, Volume 43, Issue 1, March 2002, Pages 1–9,Akihisa Takahashi*2002/3/1Akihisa Takahashi 1Department of Biology, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634–8521, Japan Corresponding author: Phone; +81–744–29–8812, Fax; +81–744–25–3345, E-mail; atakahas@naramed-u.ac.jp"1-9"We investigated whethe chonic iadiation at a low dose-ate intefees with the p53-centeed signal tansduction pathway induced by adiation in human cultued cells and C57BL/6N mice. In in vito expeiments, we found that a challenge with X-ay iadiation immediately afte chonic iadiation esulted in lowe levels of p53 than those obseved afte the challenge alone in glioblastoma cells (A-172). In addition, the levels of p53-centeed apoptosis and its elated poteins afte the challenge wee stongly coelated with the above-mentioned phenomena in squamous cell cacinoma cells (SAS/neo). In in vivo expeiments, the accumulation of p53 and Bax, and the induction of apoptosis wee obseved dose-dependently in mouse spleen at 12 h afte a challenge with X-ays (3.0 Gy). Howeve, we found significant suppession of p53 and Bax accumulation and the induction of apoptosis 12 h afte challenge iadiation at 3.0 Gy with a high dose-ate following chonic pe-iadiation (1.5 Gy, 0.001 Gy/min). These findings suggest that chonic pe-iadiation suppessed the p53 function though adiation-induced signaling and/o p53 stability.
Journal of Radiation Research2002Volume 43, Issue 1, MarchSignificance of the Response of Quiescent Cell Populations within Solid Tumors in Cancer Therapyhttps://doi.org/10.1269/jrr.43.11Journal of Radiation Research, Volume 43, Issue 1, March 2002, Pages 11–25,Shin-Ichiro Masunaga*; Koji Ono2002/3/1Shin-Ichiro Masunaga 1Radiation Oncology Research Laboratory, Research Reactor Institute, Kyoto University, Noda, Kumatori-cho, Sennan-gun, Osaka 590–0494, Japan Corresponding author: Phone; +81–724–51–2406, 2487, 2475, Fax; +81–724–51–2627, E-mail; smasuna@rri.kyoto-u.ac.jp; Koji Ono 1Radiation Oncology Research Laboratory, Research Reactor Institute, Kyoto University, Noda, Kumatori-cho, Sennan-gun, Osaka 590–0494, Japan"11-25"In analyzing the esponse of quiescent (Q) cells in solid tumos, we have developed a combined method with a miconucleus (MN) assay and the identification of polifeating (P) cells by 5-bomo-2'-deoxyuidine (BdU) and an anti-BdU monoclonal antibody. Using this method, the esponses of Q tumo cells as well as total tumo (P+Q) cells within muine solid tumos to vaious DNA-damaging teatments wee evaluated. Based on this evaluation, combining with tiapazamine, a well-known bioeductive agent, and/o heat teatment at mild tempeatues was thought to be a pomising modality fo cance theapy in tems of conventional anticance teatment-esistant Q cell contol. Recently, ou method fo detecting the Q-cell esponse using P cell labeling with BdU and the MN fequency assay was also shown to be applicable to an apoptosis detection assay. Meanwhile, ou method fo detecting the intatumo Q-cell esponse was also applicable towad high linea enegy tansfe adiation, including eacto neutons. Thus, using ou method, a new neuton captue compound that has the potential to be distibuted in neuton captue theapy-esistant intatumo Q cell populations is now unde development.
Journal of Radiation Research2002Volume 43, Issue 1, MarchIn Vitro Radiation-induced Effects on Rat Tracheal Epithelial CellsI) Different Radiosensitivity of Cell Inactivation after α and γ Irradiationshttps://doi.org/10.1269/jrr.43.27Journal of Radiation Research, Volume 43, Issue 1, March 2002, Pages 27–34,Carole Kugel; Isabelle Bailly; Fran??oise Tourdes; Jean-Luc Poncy*2002/3/1Carole Kugel 1CEA/DSV/DRR/SRCA/Laboratoire de RadioToxicologie BP 12 91680 Bruyères-le-Ch??tel, France; Isabelle Bailly 1CEA/DSV/DRR/SRCA/Laboratoire de RadioToxicologie BP 12 91680 Bruyères-le-Ch??tel, France; Fran??oise Tourdes 1CEA/DSV/DRR/SRCA/Laboratoire de RadioToxicologie BP 12 91680 Bruyères-le-Ch??tel, France; Jean-Luc Poncy 1CEA/DSV/DRR/SRCA/Laboratoire de RadioToxicologie BP 12 91680 Bruyères-le-Ch??tel, France Corresponding author: Phone; (+) 33.1–69–26–63–91, Fax; (+) 33.1–69–26–70–45, E-mail; jean-luc.poncy@cea.fr"27-34"In ode to compae the adiotoxicity of alpha- and gamma-iadiations, pimay cultues of tacheal epithelial cells fom two at stains, Spague Dawley (SD) and Wista Futh/Fishe F344 (WF/Fi) ats, wee iadiated with 241Am α-paticles o 60Co γ-ays.The suvival atio fo each of the two at stain cells appeaed to be statistically diffeent afte high-LET iadiation. WF/Fi at cells wee 1.7-times moe adiosensitive than SD at cells, wheeas no diffeence was obseved following low-LET iadiation. A compaison of the cell suvival yielded RBEs of 2.8 and 4.5 fo SD and WF/Fi at cells, espectively. As peviously obseved, with inceasing LET of paticles, the cell-suvival cuves appoximate an exponential function of the dose. On the contay, fo low-LET, the suvival cuves showed a maked initial shoulde.This in vito cellula model, using epithelial cells of the uppe aiway, povides a suitable system to estimate the mechanism involved in adiosensitivity afte high-LET iadiation. The esponses to adiation-induced lethal effects within a same type of cell wee dependent on the iadiation paametes, but might be modulated by the individual sensitivity unde genetic o epigenetic facto contols.
Journal of Radiation Research2002Volume 43, Issue 1, MarchIn Vitro Radiation-induced Effects on Rat Tracheal Epithelial Cells II) Different Preneoplastic Cell Transformation After α and γ Irradiationshttps://doi.org/10.1269/jrr.43.35Journal of Radiation Research, Volume 43, Issue 1, March 2002, Pages 35–42,Jean-Luc Poncy*; Carole Kugel; Fran??oise Tourdes; Isabelle Bailly2002/3/1Jean-Luc Poncy 1CEA/DSV/DRR/SRCA/ Laboratoire de RadioToxicologie BP 12 91680 Bruyères-le-Ch??tel, France Corresponding author: Phone; (+) 33.1–69–26–63–91, Fax; (+) 33.1–69–26–70–45, E-mail: jean-luc.poncy@cea.fr; Carole Kugel 1CEA/DSV/DRR/SRCA/ Laboratoire de RadioToxicologie BP 12 91680 Bruyères-le-Ch??tel, France; Fran??oise Tourdes 1CEA/DSV/DRR/SRCA/ Laboratoire de RadioToxicologie BP 12 91680 Bruyères-le-Ch??tel, France; Isabelle Bailly 1CEA/DSV/DRR/SRCA/ Laboratoire de RadioToxicologie BP 12 91680 Bruyères-le-Ch??tel, France"35-42"In ode to compae the cell tansfomation induced by α- and γ-iadiation, pimay cultues of tacheal epithelial cells fom two at stains, Spague Dawley (SD) and Wista Futh / Fishe F344 (WF/Fi) ats, wee iadiated with 241Am α-paticles o 60Co γ-ays.The elative tansfomation fequency (RTF) fo WF/Fi pimay cells was vey close to the level of the spontaneous incidence and independent on the two iadiation types used. On the contay, the RTF fo the SD pimay cells inceased with a decease of the LET adiation when the elative suvival was highe than about 40%. Theefoe, the RTF values eached 4–5 fo α-paticles and 10–12 fo γ-ays.The RTF can be elated to the intinsic adiosensitivity of the at epithelial cells. Howeve, the diffeence in the adiation-induced RTF fo SD o WF/Fi pimay cells seems to be due to the development, unde genetic contol, of the initial lesion to the neoplastic state.
Journal of Radiation Research2002Volume 43, Issue 1, MarchA Nitroimidazole Derivative, PR-350, Enhances the Killing of Pancreatic Cancer Cells Exposed to High-dose Irradiation under Hypoxiahttps://doi.org/10.1269/jrr.43.43Journal of Radiation Research, Volume 43, Issue 1, March 2002, Pages 43–51,Kazuhiro Mizumoto*; Li-Wu Qian; Li Zhang; Eishi Nagai; Shinobu Kura; Masao Tanaka2002/3/1Kazuhiro Mizumoto 1Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812–8582, Japan Corresponding author: Phone; +81–92–642–5444, Fax; +81–92–642–5458, E-mail; mizumoto@mailserver, www.med.kyushu-u.ac.jp/; Li-Wu Qian 1Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812–8582, Japan; Li Zhang 1Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812–8582, Japan; Eishi Nagai 1Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812–8582, Japan; Shinobu Kura 2Institute of Experimental Radiology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812–8582, Japan; Masao Tanaka 1Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812–8582, Japan"43-51"The adiosensitizing effects of PR-350, a nitoimidazole deivative, wee examined concening the cell killing of human panceatic cance cell lines exposed to high doses of gamma-ay iadiation in vito. The pecentages of dead cells wee analyzed with a multiwell plate eade to measue the fluoescence intensity of popidium iodide befoe and afte a digitonin teatment. The sensitizing effect of PR-350 on cell killing by high-dose iadiation was confimed by time-couse, dose-dependency, and micoscopic obsevations. In five of seven panceatic cance cell lines in which the numbe of dead cells was detemined 5 days afte 30 Gy iadiation in the pesence of PR-350, the numbe was significantly inceased unde hypoxic conditions, but not unde aeobic conditions. The selective adiosensitive effect of PR-350 on hypoxic cells was also confimed by flow cytomety. The esults indicate that PR-350 can enhance the killing of panceatic cance cells by high-dose iadiation unde hypoxia, which suppots its clinical adiosensitizing effects when administeed duing intaopeative iadiation to panceatic cance.
Journal of Radiation Research2002Volume 43, Issue 1, MarchReassessment of the Cancer Mortality Risk among Hiroshima Atomic-Bomb Survivors Using a New Dosimetry System, ABS2000D, Compared with ABS93Dhttps://doi.org/10.1269/jrr.43.53Journal of Radiation Research, Volume 43, Issue 1, March 2002, Pages 53–63,Hiroaki Katayama*; Masaaki Matsuura; Satoru Endo; Masaharu Hoshi; Megu Ohtaki; Norihiko Hayakawa2002/3/1Hiroaki Katayama 1Department of Information Technology, Radiation Effects Research Foundation, 5–2 Hijiyama-koen, Minami-ku, Hiroshima 732–0815, Japan Corresponding author: Phone; +81–82–261–1953, FAX; +81–82–261–4086, E-mail; H_katayama@rerf.jp; Masaaki Matsuura 2Department of Environmetrics and Biometrics, Division of Social Medicine, Research Institute for Radiation Biology and Medicine, Hiroshima University, 1–2–3 Kasumi, Minami-ku, Hiroshima 734–8553, Japan; Satoru Endo 3Applied Physics and Chemistry, Graduate School of Engineering, Hiroshima University, 739–8527, Japan; Masaharu Hoshi 4International Radiation Information Center, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima 734–8553, Japan; Megu Ohtaki 2Department of Environmetrics and Biometrics, Division of Social Medicine, Research Institute for Radiation Biology and Medicine, Hiroshima University, 1–2–3 Kasumi, Minami-ku, Hiroshima 734–8553, Japan; Norihiko Hayakawa 5Department of Epidemiology, Division of Social Medicine, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima 734–8553, Japan"53-63"The aim of the pesent study was to examine the excess elative isk fo leukemia motality and all cances, except leukemia, among Hioshima atomic-bomb suvivos by applying ABS93D and ABS2000D. Paticula attention was given to any diffeence in the neuton-dose estimates between the two dosimety systems. The study subjects wee 51,532 atomic-bomb suvivos egisteed in a database of the Reseach Institute fo Radiation Biology and Medicine of Hioshima Univesity (RIRBM). The esults obtained by both dosimety systems ae simila: the excess elative isk pe Sv fo leukemia motality and all cances except leukemia is significantly highe compaed to the contol goup. In addition, the diffeence in the excess elative isks between the two systems is not significant. Theefoe, it is indicated that a modification of the neuton-dose estimates would not makedly change the conclusions about the cance motality isk.
Journal of Radiation Research2002Volume 43, Issue 1, MarchThe Quality of DNA Recovered from the Archival Tissues of Atomic Bomb Survivors is Good Enough for the Single Nucleotide Polymorphism Analysis in Spite of the Decade-long Preservation in Formalinhttps://doi.org/10.1269/jrr.43.65Journal of Radiation Research, Volume 43, Issue 1, March 2002, Pages 65–75,Yumiko Nitta*; Hideo Tanaka; Yuji Masuda; Masaharu Hoshi2002/3/1Yumiko Nitta 1International Radiation Information Center, Research Institute for Radiation Biology and Medicine, Hiroshima University, Kasumi 1–2–3, Minami-Ku, Hiroshima 734–8553, Japan Corresponding author: Phone; 082–257–5875, Fax: 082–257–5877, E-mail; yumiko@hiroshima-u.ac.jp; Hideo Tanaka 2Department of Hematology and Oncology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Kasumi 1–2–3, Minami-Ku, Hiroshima 734–8553, Japan; Yuji Masuda 3Department of Developmental Biology and Oncology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Kasumi 1–2–3, Minami-Ku, Hiroshima 734–8553, Japan; Masaharu Hoshi 1International Radiation Information Center, Research Institute for Radiation Biology and Medicine, Hiroshima University, Kasumi 1–2–3, Minami-Ku, Hiroshima 734–8553, Japan"65-75"It is well known that the yield of DNA ecoveed fom tissues peseved in fomalin is invesely popotional to the stoed duation. How is the quality? We tested the quality of DNA fom achival tissues of atomic-bomb suvivos stoed in fomalin fo decades with the paametes of gene amplification efficiency by a polymease chain eaction. All of the DNA extacted fom the tissues peseved in fomalin fo 30 yeas amplified the 54-and 61-base pais of the DNA fagments successfully. The diect sequencing of the PCR poducts confimed the accuate amplification of the taget sequence. A futhe tial to amplify the longe sequence of 111 base pais succeeded in 20% of the samples tested. Fom these esults, we popose a new utility of achival samples fo the analysis of single nucleotide sequence polymophism of genes, no matte how long the samples have been peseved in fomalin.
Journal of Radiation Research2002Volume 43, Issue 1, MarchRadiation-chemical Properties of the Hypoxic Cell Radiosensitizer Doranidazole (PR-350)https://doi.org/10.1269/jrr.43.77Journal of Radiation Research, Volume 43, Issue 1, March 2002, Pages 77–88,Mikinori Kuwabara*; Yoshiharu Iida; Osamu Inanami; Sadashi Sawamura; Kouji Yokoyama; Michihiko Tsujitani2002/3/1Mikinori Kuwabara 1Laboratory of Radiation Biology, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060–0818, Japan Corresponding author: Phone; +81–11–706–5235, Fax; +81–11–706–7373, E-mail; kuwabara@vetmed.hokudai.ac.jp; Yoshiharu Iida 1Laboratory of Radiation Biology, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060–0818, Japan; Osamu Inanami 1Laboratory of Radiation Biology, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060–0818, Japan; Sadashi Sawamura 2Laboratory of Applied Radiation Science, Graduate School of Engineering, Hokkaido University, Sapporo 060–8628, Japan; Kouji Yokoyama 3Pola Chemical Industries, Inc., Yokohama 221–0833, Japan; Michihiko Tsujitani 3Pola Chemical Industries, Inc., Yokohama 221–0833, Japan"77-88"This study was pefomed to confim the adiation-chemical popeties of the 2-nitoimidazole deivative doanidazole, (±)-(2RS,3SR)-3-(2-nitoimdazol-1-yl)-methoxybutane-1,2,4-tiol CAS 137339–64–1, PR-350, which was synthesized as a hypoxic cell adiosensitize with low toxicity. Radiation-chemical expeiments using doanidazole showed that (1) unlike O2, it had high eactivity towad not only hydated electons (eaq-), but also hydoxyl adicals (·OH), (2) the educed intemediates of doanidasole had no ability to induce immediate stand beaks of colE1 plasmid DNA, (3) doanidazole enhanced adiation-induced DNA stand beaks of colE1 plasmid DNA in the aqueous state, wheeas it did not enhance the base alteation, such as 8-oxo-deoxyguanosine, (4) it enhanced the adiation-induced fomation of stand beaks with 3'-phosophate and 3'- phosphoglycolate temini, and (5) it was bound to DNA afte iadiation. These facts evealed that the majoity of adiation-chemical popeties of doanidazole, except fo the high eactivity towad ·OH, wee simila to those of oxygen.
Journal of Radiation Research2002Volume 43, Issue 1, MarchTypes and Three-dimensional Distribution of Neuronal Ectopias in the Brain of Mice Prenatally Subjected to X-irradiationhttps://doi.org/10.1269/jrr.43.89Journal of Radiation Research, Volume 43, Issue 1, March 2002, Pages 89–98,Xue-Zhi Sun*; Sentaro Takahashi; Yoshihisa Kubota; Hiroshi Sato; Chun Cui; Yoshihiro Fukui; Minoru Inouye2002/3/1Xue-Zhi Sun 1Environmental and Toxicological Sciences Research Group, National Institute of Radiological Sciences, 4–9–1, Inage-ku, Chiba 263–8555, Japan Corresponding author: Phone; +81–43–206–3159, Fax; +81–43–251–4853, E-mail; sun_s@nirs.go.jp; Sentaro Takahashi 1Environmental and Toxicological Sciences Research Group, National Institute of Radiological Sciences, 4–9–1, Inage-ku, Chiba 263–8555, Japan; Yoshihisa Kubota 1Environmental and Toxicological Sciences Research Group, National Institute of Radiological Sciences, 4–9–1, Inage-ku, Chiba 263–8555, Japan; Hiroshi Sato 1Environmental and Toxicological Sciences Research Group, National Institute of Radiological Sciences, 4–9–1, Inage-ku, Chiba 263–8555, Japan; Chun Cui 2Department of Anatomy, School of Medicine, Tokushima University, 3–18–15 Kuramoto-cho, Tokushima 770–8503, Japan; Yoshihiro Fukui 2Department of Anatomy, School of Medicine, Tokushima University, 3–18–15 Kuramoto-cho, Tokushima 770–8503, Japan; Minoru Inouye 3Shin Nippon Biomedical Laborarories, Ltd. 2438 Miyanoura, Yoshida, Kagoshima 891–1394, Japan"89-98"The types and thee-dimensional distibution of neocotical ectopias following penatal exposue to X-iadiation wee studied by a histological examination and compute econstuction techniques. Pegnant ICR mice wee subjected to X-iadiation at a dose of 1.5 Gy on embyonic day 13. The bains fom 30-day-old mice wee seially sectioned on the fontal plane at 15 μm, stained with HE and obseved with a micoscope. The image data fo the sections wee input to a compute, and then econstucted to thee-dimensional bain stuctues using the Magellan 3.6 pogam. Sectional images wee then dawn on a compute display at 240 μm intevals, and the positions of the diffeent types of neocotical ectopias wee maked using colo coding. Thee types of neocotical ectopias wee ecognized in the iadiated bains. Neocotical Lay I ectopias wee identified as small patches in the caudal occipital cotex, and wee located moe lateally in the neocotex in caudal sections than in the ostal sections. Peiventicula ectopias wee located moe ostally than Lay I ectopias, and wee found fom the most caudal extent of the pesumed moto cotex to the most caudal extent of the lateal venticle. Hippocampal ectopias appeaed as continuous linea bands, and wee fequently associated with the anteio pats of the peiventicula ectopias.
Journal of Radiation Research2002Volume 43, Issue 1, MarchRedox Reactions of Tocopherol Monoglucoside in Aqueous Solutions: A Pulse Radiolysis Studyhttps://doi.org/10.1269/jrr.43.99Journal of Radiation Research, Volume 43, Issue 1, March 2002, Pages 99–106,Sudhir Kapoor*; Tulsi Mukherjee; Tsutomu V. Kagiya; Cherupally Krishnan K. Nair2002/3/1Sudhir Kapoor 1Radiation Chemistry Chemical Dynamics Division, Bhabha Atomic Research Centre, Mumbai 400 085, India Corresponding author: Phone; 91–22–5505291, FAX: 91–22–5505151, E-mail; sudhirk@apsara.barc.ernet.in; Tulsi Mukherjee 1Radiation Chemistry Chemical Dynamics Division, Bhabha Atomic Research Centre, Mumbai 400 085, India; Tsutomu V. Kagiya 2Health Research Foundation, Kyoto 606–8225, Japan; Cherupally Krishnan K. Nair 3Radiation Biology Division, Bhabha Atomic Research Centre, Mumbai 400 085, India"99-106"The eactions between tocopheol monoglucoside (TMG), a wate-soluble vitamin-E deivative, with B2· -, N3·, (SCN)2·-, NO2·, OH· and vaious halogenated peoxyl adicals wee examined using a pulse adiolysis technique. The esults demonstate that TMG foms a stable phenoxyl adical at pH > 6.8. The thus-fomed phenoxyl adical shows pH-dependent decay kinetics and is dispopotionated by 2nd ode kinetics at pH 2.3. It was obseved that the TMG eactivity towads a halogenated peoxyl adical inceases with the numbe of halogen atoms at the cabon atom having a peoxyl goup. The eaction between the TMG phenoxyl adical and ascobic acid was also examined using a pulse adiolysis technique. The esults indicate that the TMG phenoxyl adical is epaied by ascobate. Kinetic studies indicate that TMG may act as an antioxidant to epai fee-adical damage to some biologically impotant compounds. The one-electon eduction potential fo TMG was found to be 0.522 V ± 0.06 vs. NHE.
Journal of Radiation Research2002Volume 43, Issue 2, JuneCytogenetic Changes in the Liver of Progeny of Irradiated Male Ratshttps://doi.org/10.1269/jrr.43.125Journal of Radiation Research, Volume 43, Issue 2, June 2002, Pages 125–133,Katarína Kropá??ová; Lucia Slovinská*; Eva Mi??úrová2002/6/1Katarína Kropá??ová 1Department of Cellular and Molecular Biology, Faculty of Sciences, P. J. ??afárik University, Moyzesova 11, 04167 Ko??ice, Slovakia; Lucia Slovinská 1Department of Cellular and Molecular Biology, Faculty of Sciences, P. J. ??afárik University, Moyzesova 11, 04167 Ko??ice, Slovakia Corresponding author: Phone: 04215562 22610, Fax: 04215562 22124, E-mail: slovins@kosice.upjs.sk; Eva Mi??úrová 1Department of Cellular and Molecular Biology, Faculty of Sciences, P. J. ??afárik University, Moyzesova 11, 04167 Ko??ice, Slovakia"125-133"The tansgeneational tansmission of adiation damage of at genom was studied on the basis of cytogenetic changes in somatic cells (hepatocytes). It was found, that the iadiation of at males with dose of 3 Gy of gamma adiation caused latent cytogenetic damage to the live, which was expessed duing the couse of an induced polifeation of hepatocytes (by patial hepatectomy) by lowe polifeative activity and a high fequency of chomosomal abeations. In the pogeny of iadiated males (in the F1 geneation), the adiation damage to DNA was manifested by simila changes, i.e. by lowe polifeation activity and incease in “spontaneus” chomosomal abeation occuence in live egeneation afte patial hepatectomy. Iadiating the pogeny of iadiated males (the total adiation load of the pogeny being 3 Gy + 3 Gy) caused slighte changes in compaed with iadiating the pogeny of non-iadiated contol males (the total adiation load of the pogeny being 0 Gy + 3 Gy), which suggests some kind of adaptive esponse, which was also found in othe expeimental systems and paametes. An analogous couse of RNA and DNA quantitative changes in the live of the F0 and F1 geneations of ats confims the patial tansmission of adiation damage of genom to the pogeny.
Journal of Radiation Research2002Volume 43, Issue 2, JuneEstablishment of an Animal Model for Radiation-induced Vomiting in Rats Using Picahttps://doi.org/10.1269/jrr.43.135Journal of Radiation Research, Volume 43, Issue 2, June 2002, Pages 135–141,Kouichi Yamamoto; Noriaki Takeda; Atsushi Yamatodani*2002/6/1Kouichi Yamamoto 1Department of Medical Physics, School of Allied Health Sciences, Faculty of Medicine, Osaka University, 1–7, Yamadaoka, Suita, Osaka 565–0871, Japan; Noriaki Takeda 2Department of Otorhinolaryngology, School of Medicine, University of Tokushima, 3–18–15, Kuramoto-cho, Tokushima 770–8503, Japan; Atsushi Yamatodani 1Department of Medical Physics, School of Allied Health Sciences, Faculty of Medicine, Osaka University, 1–7, Yamadaoka, Suita, Osaka 565–0871, Japan Corresponding author: Phone: +81–6–6879–2562, Fax: +81–6–6879–2562, E-mail: yamatoda@sahs.med.osaka-u.ac.jp"135-141"We investigated whethe adiation-induced pica, a behavio chaacteized by the eating of a non-food substance, such as kaolin, can be used as an index of adiation-induced vomiting in ats. Since thee was an individual diffeence in the susceptibility to pica, we selected ats that actually ate kaolin following X-ay iadiation, and used them fo the expeiment. The total-body iadiation (TBI) inceased kaolin consumption in a dose-dependent manne (sham, 0.05 ± 0.03 (SEM) g; 2 Gy, 0.38 ± 0.11 g; 4 Gy, 1.54 ± 0.28 g; 8 Gy, 3.55 ± 0.67 g), and the inceased kaolin consumption afte 4 Gy of TBI was inhibited by a peteatment with the seotonin 5-HT3 ecepto antagonist ondanseton (2 mg/kg, i.p.) (saline, 1.49 ± 0.33 g; ondanseton, 0.75 ± 0.11 g). Futhemoe, 4 Gy of abdominal iadiation was moe effective to induce pica than that of head iadiation (abdomen: 0.37 ± 0.05 g, head: 0.06 ± 0.01 g). These findings suggested that peipheal seotonegic pathway is pedominantly involved in the development of adiation-induced pica in ats and that the adiation-induced pica could be useful as a behavioal index fo the seveity of adiation-induced vomiting in ats.
Journal of Radiation Research2002Volume 43, Issue 2, JuneHistological and Elemental Changes in the Rat Brain after Local Irradiation with Carbon Ion Beamshttps://doi.org/10.1269/jrr.43.143Journal of Radiation Research, Volume 43, Issue 2, June 2002, Pages 143–152,Sentaro Takahashi*; Xue-Zhi Sun; Yoshihisa Kubota; Nobuhiko Takai; Kumie Nojima2002/6/1Sentaro Takahashi 1Environmental and Toxicological Research Group, National Institute of Radiological Sciences Anagawa 4–9–1, Inage-ku, Chiba-shi, Chiba 263–8555, Japan Corresponding author: Phone: +81–43–206–3158, Fax: +81–43–251–4853, E-mail: sentaro@nirs.go.jp; Xue-Zhi Sun 1Environmental and Toxicological Research Group, National Institute of Radiological Sciences Anagawa 4–9–1, Inage-ku, Chiba-shi, Chiba 263–8555, Japan; Yoshihisa Kubota 1Environmental and Toxicological Research Group, National Institute of Radiological Sciences Anagawa 4–9–1, Inage-ku, Chiba-shi, Chiba 263–8555, Japan; Nobuhiko Takai 2Research Laboratory of Space Radiation, National Institute of Radiological Sciences Anagawa 4–9–1, Inage-ku, Chiba-shi, Chiba 263–8555, Japan; Kumie Nojima 2Research Laboratory of Space Radiation, National Institute of Radiological Sciences Anagawa 4–9–1, Inage-ku, Chiba-shi, Chiba 263–8555, Japan"143-152"The left ceebal hemisphees of adult Spague-Dawley at bains wee iadiated at doses of 30, 50, o 100 Gy with chaged cabon paticles (290 MeV/nucleon; 5 mm spead-out Bagg peak). The spead-out Bagg peak used hee successfully and satisfactoily etained its high-dose localization in the defined egion. A histological examination showed that necotic tissue damage, hemohage in the thalamus, and vasodilatations aound the necotic egion wee induced at 8 weeks afte 100 Gy iadiation. The egions with tissue damage coelated well with those expected fom the adiation-dose distibution, indicating an advantage of chaged cabon paticles fo iadiating esticted bain egions. An X-ay fluoescent analysis demonstated a decease in the concentations of K and P, and an incease in the concentations of Cl, Fe, Zn in the damaged egion at 8 weeks post-iadiation, though no significant changes wee obseved befoe 4 weeks of post-iadiation. This may indicate that even the vey high adiation doses used hee did not induce acute and immediate neuonal cell death, in contast with ischemic bain injuy whee acute neuonal cell death occued and the elemental concentations changed within a day afte the induction of ischemia.
Journal of Radiation Research2002Volume 43, Issue 2, JuneInhibition of γ-Radiation Induced DNA Damage in Plasmid pBR322 by TMG, a Water-soluble Derivative of Vitamin Ehttps://doi.org/10.1269/jrr.43.153Journal of Radiation Research, Volume 43, Issue 2, June 2002, Pages 153–159,Rema Rajagopalan; Khalida Wani; Nagaraj G. Huilgol; Tsutomu V. Kagiya; Cherupally K. Krishnan Nair*2002/6/1Rema Rajagopalan 1Radiation Biology Division, Bhabha Atomic Research Centre, Mumbai 400 085; Khalida Wani 2Department of Radiation Oncology, Nanavati hospital MRC, Vile Parle, India; Nagaraj G. Huilgol 2Department of Radiation Oncology, Nanavati hospital MRC, Vile Parle, India; Tsutomu V. Kagiya 3Kinki Research Foundation, Kyoto, Japan; Cherupally K. Krishnan Nair 1Radiation Biology Division, Bhabha Atomic Research Centre, Mumbai 400 085 Corresponding author: Phone: 91–22–5593869, Fax: 91–22–5560750/91–22–5505151, E-mail: cknair@magnum.barc.ernet.in"153-159"Alpha-tocopheol monoglucoside (TMG), a wate-soluble deivative of α-tocopheol, has been examined fo its ability to potect DNA against adiation-induced stand beaks. Gamma adiation, up to a dose of 6 Gy (dose ate, 0.7 Gy/ minute), induced a dose-dependent incease in single stand beaks (SSBs) in plasmid pBR322 DNA. TMG inhibited the fomation of γ-adiation induced DNA single stand beaks (SSBs) in a concentation-dependent manne; 500 μM of TMG potected the single stand beaks completely. It also potected thymine glycol fomation induced by γ-adiation in a dose-dependent manne, based on an estimation of thymine glycol by HPLC.
Journal of Radiation Research2002Volume 43, Issue 2, JuneEffect of a Hypoxic Cell Sensitizer Doranidazole on the Radiation-induced Apoptosis of Mouse L5178Y Lymphoma Cellshttps://doi.org/10.1269/jrr.43.161Journal of Radiation Research, Volume 43, Issue 2, June 2002, Pages 161–166,Mizuho Aoki*; Yoshiya Furusawa; Yuta Shibamoto; Ataru Kobayashi; Michihiko Tsujitani2002/6/1Mizuho Aoki 1Research Center for Charged Particle Therapy, National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba 263–8555, Japan Corresponding author: Phone: +81–43–206–3232, Fax: +81–43–206–4149, E-mail: mizuho@nirs.go.jp; Yoshiya Furusawa 2Department of Therapeutic Radiology and Oncology, Graduate School of Medicine, Kyoto University, 54 Shogoinkawara-machi, Sakyo-ku, Kyoto 606–8507, Japan; Yuta Shibamoto 2Department of Therapeutic Radiology and Oncology, Graduate School of Medicine, Kyoto University, 54 Shogoinkawara-machi, Sakyo-ku, Kyoto 606–8507, Japan; Ataru Kobayashi 3Pharmaceuticals Division, Pola Chemical Industries, Inc., 560 Kashiocho Totsuka-ku, Yokohama, Kanagawa 244–0814, Japan; Michihiko Tsujitani 4Pola Chemical Industries, Inc., 27–1 Takashimadai, Kanagawa-ku, Yokohama 221–0833, Japan"161-166"We investigated the sensitizing effect of the 2-nitoimidazole analogue doanidazole, a new hypoxic adiosensitize, on adiation-induced apoptosis in L5178Y cells. Apoptosis was assessed by checking DNA ladde fomation, the pesence of sub-G1 peaks in flow cytomety, and chomatin condensation. A adiosensitizing effect of doanidazole was also confimed by a soft-aga colony assay of suviving cells. In the assay of DNA ladde fomation, DNA fagmentation was obseved following iadiation unde an aeobic o hypoxic condition with o without doanidazole. The popotions of the cells at the sub-G1 peak in a flow cytometic measuement was not vey diffeent among the iadiations at 5 Gy unde the aeobic condition, 15 Gy unde hypoxia, and 10 Gy with 1 mM doanidazole unde hypoxia. The faction of cells with chomatin condensation was found to be significantly inceased with doanidazole up to 3 mM when applied unde hypoxic iadiation, but did not incease even at 10 mM. The sensitize enhancement atio was estimated to be about 1.7 with a concentation of 1 mM. This enhancement atio was not diffeent fom that obseved by assaying cell suvivals. On the othe hand, doanidazole showed no adiosensitizing effect unde aeobic conditions with 1 mM. In conclusion, the adiation-induced apoptosis of L5178Y cells was enhanced by doanidazole unde hypoxia.
Journal of Radiation Research2002Volume 43, Issue 2, JuneEstablishment of Immortal Normal and Ataxia Telangiectasia Fibroblast Cell Lines by Introduction of the hTERT Genehttps://doi.org/10.1269/jrr.43.167Journal of Radiation Research, Volume 43, Issue 2, June 2002, Pages 167–174,Hideaki Nakamura*; Hiroko Fukami; Yuko Hayashi; Tohru Kiyono; Shigekazu Nakatsugawa; Michinari Hamaguchi; Kanji Ishizaki2002/6/1Hideaki Nakamura 1Central Laboratory and Radiation Biology, Aichi Cancer Center Research Institute, Kanokoden 1–1, Chikusa-ku, Nagoya 464–8681, Japan2Department of Molecular Pathogenesis, Nagoya University School of Medicine, Tsurumai-cho 65, Showa-ku, Nagoya 466–8550, Japan Corresponding author: Phone: +81–52–762–6111 (ext. 7012), Fax: +81–52–763–5233, E-mail: hnakamu@aichi-cc.jp; Hiroko Fukami 1Central Laboratory and Radiation Biology, Aichi Cancer Center Research Institute, Kanokoden 1–1, Chikusa-ku, Nagoya 464–8681, Japan; Yuko Hayashi 1Central Laboratory and Radiation Biology, Aichi Cancer Center Research Institute, Kanokoden 1–1, Chikusa-ku, Nagoya 464–8681, Japan; Tohru Kiyono 3Division of Virology, Aichi Cancer Center Research Institute, Kanokoden 1–1, Chikusa-ku, Nagoya 464–8681, Japan; Shigekazu Nakatsugawa 4Department of Radiology, Nagoya University School of Medicine, Tsurumai-cho 65, Showa-ku, Nagoya 466–8550, Japan; Michinari Hamaguchi 2Department of Molecular Pathogenesis, Nagoya University School of Medicine, Tsurumai-cho 65, Showa-ku, Nagoya 466–8550, Japan; Kanji Ishizaki 1Central Laboratory and Radiation Biology, Aichi Cancer Center Research Institute, Kanokoden 1–1, Chikusa-ku, Nagoya 464–8681, Japan"167-174"To establish immotal human cells, we intoduced the human catalytic subunit of telomease (hTERT) gene into skin fiboblast cells obtained fom nomal and ataxia telangiectasia (AT) individuals of Japanese oigin. Afte hTERT intoduction, these cells continue to gow beyond a population doubling numbe of 200 while maintaining thei oiginal adiosensitivity. Inductions of p53, phosphoylation of Se15 in p53, and induction of p21 by X-ay iadiation in immotal cells deived fom nomal individual wee not affected by the hTERT intoduction. Both nomal and AT immotal cells exhibited an appaent inhibition of gowth as oiginal pimay cells when they eached confluence. Kayotype analysis has evealed that they ae in a diploid ange. These esults suggest that cells immotalized by hTERT intoduction etain thei oiginal chaacteistics except fo immotalization, and that they may be useful fo analyzing vaious effects of adiation on human cells.
Journal of Radiation Research2002Volume 43, Issue 2, JunePutative Tumor-suppressor Gene Regions Responsible for Radiation Lymphomagenesis in F1 Mice with Different p53 Statushttps://doi.org/10.1269/jrr.43.175Journal of Radiation Research, Volume 43, Issue 2, June 2002, Pages 175–185,Doo-Pyo Hong; Nobuko Mori; Seiichi Umesako; Chang-Woo Song; Yeong-Gwan Park; Shiro Aizawa; Masaaki Okumoto*2002/6/1Doo-Pyo Hong 1Research Institute for Advanced Science and Technology, Osaka Prefecture University, 1–2 Gakuen-cho, Sakai, Osaka 599–8570, Japan2Graduate school of Agriculture and Biological Sciences, Osaka Prefecture University, 1–1 Gakuen-cho, Sakai, Osaka 599–8531, Japan; Nobuko Mori 1Research Institute for Advanced Science and Technology, Osaka Prefecture University, 1–2 Gakuen-cho, Sakai, Osaka 599–8570, Japan; Seiichi Umesako 1Research Institute for Advanced Science and Technology, Osaka Prefecture University, 1–2 Gakuen-cho, Sakai, Osaka 599–8570, Japan2Graduate school of Agriculture and Biological Sciences, Osaka Prefecture University, 1–1 Gakuen-cho, Sakai, Osaka 599–8531, Japan; Chang-Woo Song 3Korea Research Institute of Chemical Technology, P.O.Box 107, Yusong, Taejon 305–606, Korea; Yeong-Gwan Park 1Research Institute for Advanced Science and Technology, Osaka Prefecture University, 1–2 Gakuen-cho, Sakai, Osaka 599–8570, Japan2Graduate school of Agriculture and Biological Sciences, Osaka Prefecture University, 1–1 Gakuen-cho, Sakai, Osaka 599–8531, Japan; Shiro Aizawa 4Division of Biology and Oncology, National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba 263–8555, Japan; Masaaki Okumoto 1Research Institute for Advanced Science and Technology, Osaka Prefecture University, 1–2 Gakuen-cho, Sakai, Osaka 599–8570, Japan2Graduate school of Agriculture and Biological Sciences, Osaka Prefecture University, 1–1 Gakuen-cho, Sakai, Osaka 599–8531, Japan Corresponding author: Phone: +81 722 54 9837, Fax: +81 722 54 9837, E-mail: okumoto@riast.osakafu-u.ac.jp"175-185"Regions of allelic loss on chomosomes in many tumos of human and some expeimental animals ae geneally consideed to habo tumo-suppesso genes involved in tumoigenesis. Allelotype analyses have geatly impoved ou undestanding of the molecula mechanism of adiation lymphomagenesis. Peviously, we and othes found fequent loss of heteozygosity (LOH) on chomosomes 4, 11, 12, 16 and 19 in adiation-induced lymphomas fom seveal F1 hybid mice. To examine possible contibutions of individual tumo-suppesso genes to tumoigenesis in p53 heteozygous deficiency, we investigated the genome-wide distibution and status of LOH in adiation-induced lymphomas fom F1 mice with diffeent p53 status. In this study, we found fequent LOH (moe than 20%) on chomosomes 4 and 12 and on chomosomes 11, 12, 16 and 19 in adiation-induced lymphomas fom (STS/A X MSM/Ms)F1 mice and (STS/A X MSM/Ms)F 1-p53KO/+ mice, espectively. Low incidences of LOH (10–20%) wee also obseved on chomosomes 11 in mice with wild-type p53, and chomosomes 1, 2, 9, 17 and X in p53 heteozygous-deficient mice. The fequency of LOH on chomosomes 9 and 11 inceased in the (STS/A X MSM/Ms)F1- p53KO/+ mice. Pefeential losses of the STS-deived allele on chomosome 9 and wild-type p53 allele on chomosome 11 wee also found in the p53 heteozygous-deficient mice. Thus, the putative tumo-suppesso gene egions esponsible fo lymphomaganesis might consideably diffe due to the p53 status.
Journal of Radiation Research2002Volume 43, Issue 2, JuneGeneration of Large Homozygous Chromosomal Segments by Mitotic Recombination during Lymphomagenesis in F1 Hybrid Micehttps://doi.org/10.1269/jrr.43.187Journal of Radiation Research, Volume 43, Issue 2, June 2002, Pages 187–194,Doo-Pyo Hong; Kihei Kubo; Naomi Tsugawa; Nobuko Mori; Seiichi Umesako; Chang-Woo Song; Masaaki Okumoto*2002/6/1Doo-Pyo Hong 1Research Institute for Advanced Science and Technology, Osaka Prefecture University, 1–2 Gakuen-cho, Sakai, Osaka 599–8570, Japan2Graduate school of Agriculture and Biological Sciences, Osaka Prefecture University, 1–1 Gakuen-cho, Sakai, Osaka 599–8531, Japan; Kihei Kubo 2Graduate school of Agriculture and Biological Sciences, Osaka Prefecture University, 1–1 Gakuen-cho, Sakai, Osaka 599–8531, Japan; Naomi Tsugawa 2Graduate school of Agriculture and Biological Sciences, Osaka Prefecture University, 1–1 Gakuen-cho, Sakai, Osaka 599–8531, Japan; Nobuko Mori 1Research Institute for Advanced Science and Technology, Osaka Prefecture University, 1–2 Gakuen-cho, Sakai, Osaka 599–8570, Japan; Seiichi Umesako 1Research Institute for Advanced Science and Technology, Osaka Prefecture University, 1–2 Gakuen-cho, Sakai, Osaka 599–8570, Japan2Graduate school of Agriculture and Biological Sciences, Osaka Prefecture University, 1–1 Gakuen-cho, Sakai, Osaka 599–8531, Japan; Chang-Woo Song 3Korea Research Institute of Chemical Technology, P.O.Box 107, Yusong, Taejon 305–606, Korea; Masaaki Okumoto 1Research Institute for Advanced Science and Technology, Osaka Prefecture University, 1–2 Gakuen-cho, Sakai, Osaka 599–8570, Japan2Graduate school of Agriculture and Biological Sciences, Osaka Prefecture University, 1–1 Gakuen-cho, Sakai, Osaka 599–8531, Japan Corresponding author: Phone: +81 722 54 9837, Fax: +81 722 54 9837, E-mail: okumoto@riast.osakafu-u.ac.jp"187-194"The loss of heteozygosity (LOH) has been epoted in numeous neoplasms in both human and animals, and has often been obseved in chomosomal egions, which contain tumo-suppesso genes. We peviously found fequent LOH on chomosomes 4, 12 and 19 in adiation-induced lymphomas fom (BALB/cHeA x STS/A)F1 hybid mice by allelotype analysis at polymophic micosatellite loci. In this study, to elucidate the natue of allelic losses, we efined the loss egions on chomosomes 4, 12 and 19 of the tumos fom the F1 mice and then analyzed them cytogenetically. The esults epesent evidence of a wide ange of allelic losses owing to mitotic ecombination on chomosomes 4 and 19 in the tumos, possibly eflecting functional losses of putative tumo-suppesso genes. It is suggested that the geneation of these lage homozygous chomosomal segments pobably containing the affected genes is one of the genetic alteations esponsible fo tumoigenesis.
Journal of Radiation Research2002Volume 43, Issue 2, JuneCharacterization of Spontaneous Mutation in the ΔsoxR and SoxS Overproducing Strains of Escherichia colihttps://doi.org/10.1269/jrr.43.195Journal of Radiation Research, Volume 43, Issue 2, June 2002, Pages 195–203,Eiji Yamamura; Eun Hye Lee; Akihiro Kuzumaki; Norio Uematsu; Tatsuo Nunoshiba; Masakado Kawata; Kazuo Yamamoto*2002/6/1Eiji Yamamura 1Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Miyagi 980–8577, Japan2Mitsubishi Pharma Corporation, Chiba 292–0812, Japan; Eun Hye Lee 1Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Miyagi 980–8577, Japan3Present Address; Department of Aquatic Life Medicine, Pukyong National University, Pusan 608–737, Korea; Akihiro Kuzumaki 1Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Miyagi 980–8577, Japan; Norio Uematsu 1Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Miyagi 980–8577, Japan; Tatsuo Nunoshiba 1Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Miyagi 980–8577, Japan; Masakado Kawata 1Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Miyagi 980–8577, Japan; Kazuo Yamamoto 1Department of Biomolecular Sciences, Graduate School of Life Sciences, Tohoku University, Miyagi 980–8577, Japan Corresponding author: Phone: +81–22–217–5054, Fax: +81–22–217–5053, E-mail: yamamot@mail.cc.tohoku.ac.jp"195-203"To examine the ole of the soxRS egulon in mutagenesis, we chaacteized the spontaneous mutations occuing in the endogenous tonB gene in the ΔsoxR stain and the SoxS ovepoducing stain of Escheichia coli. Neithe the ΔsoxR stain no the SoxS ovepoducing stain led to an enhancement o diminishment of the spontaneous mutation fequency. By DNA sequencing, we detemined 50 spontaneous mutants fom the ΔsoxR stains, and found that 36% wee both base substitutions and IS insetions, 14% fameshifts and 10% deletions. Among the base substitutions, G:C→T:A tansvesions and G:C→A:T tansitions pedominated, followed by A:T→T:A tansvesions. We detemined 54 spontaneous mutants fom the SoxS ovepoducing stains, and found that 37% wee IS insetions, 31% base substitutions, 17% fameshifts, 9% deletions and 6% duplications. Among the base substitutions, G:C→T:A tansvesions dominated, followed by A:T →T:A tansvesions and G:C→A:T tansitions. These esults wee simila to those fom the soxRS+ stains. Thus, it is suggested that the soxRS-egulated genes do not play a significant ole in the defense against spontaneous mutagenesis.
Journal of Radiation Research2002Volume 43, Issue 2, JuneWhole-body X-irradiation Induces Acute and Transient Expression of Heme Oxygenase-1 in Rat Liverhttps://doi.org/10.1269/jrr.43.205Journal of Radiation Research, Volume 43, Issue 2, June 2002, Pages 205–210,Keiko Suzuki*; Masahiko Mori; Fumihiko Kugawa; Hiroshi Ishihara2002/6/1Keiko Suzuki 1Redox Regulation Research Group, National Institute of Radiological Sciences, Anagawa 4–9–1, Inage-ku, Chiba 263–8555, Japan Corresponding author: Phone: +81–43–206–3127, Fax: +81–43–255–6819, E-mail: suzuki_q@nirs.go.jp; Masahiko Mori 2Radiation Hazard Research Group, National Institute of Radiological Sciences, Anagawa 4–9–1, Inage-ku, Chiba 263–8555, Japan; Fumihiko Kugawa 3Department of Biological Pharmaceutical Sciences, College of Pharmacy, Nihon Univ., 7–7–1 Narashino-dai, Funabashi 274–8555, Japan; Hiroshi Ishihara 1Redox Regulation Research Group, National Institute of Radiological Sciences, Anagawa 4–9–1, Inage-ku, Chiba 263–8555, Japan"205-210"Activation of the stess-inducible heme oxygenase-1 (HO-1) gene by X-iadiation was investigated in at live. When male Wista MS stain ats (8 weeks) eceived whole-body iadiation of 17.0 Gy, 7 h late the activity of heme oxygenase in the live was significantly enhanced (2.5 times). The level of HO-1 mRNA expession was inceased by 2.3 and 4.0 times 2 and 4 h afte adiation, and then declined at 7 and 10 h to the level of 2.0 and 1.6 times of the contol. When the X-ay dose was vaied fom 4.0 to 21.7 Gy, the tansciption of the gene was enhanced at all doses and the level of activation was dose-dependent. Finally, westen blotting of iadiated live demonstated a significant incease in the level of HO-1 induced by X-ays, peaking at 4 h. Thus, X-ays wee confimed to be stessos that induce acute HO-1 expession tansiently in the live.
Journal of Radiation Research2002Volume 43, Issue 3, SeptemberDimerization, Translocation and Localization of Ku70 and Ku80 Proteinshttps://doi.org/10.1269/jrr.43.223Journal of Radiation Research, Volume 43, Issue 3, September 2002, Pages 223–236,Manabu Koike*2002/9/1Manabu Koike 1Radiation Hazards Research Group, National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba 263–8555, Japan Corresponding author: Phone: 81–43–206–3139, Fax: 81–43–206–3139, E-mail: m_koike@nirs.go.jp"223-236"The Ku potein is a complex of two subunits, Ku70 and Ku80, and was oiginally identified as an autoantigen ecognized by the sea of patients with autoimmune diseases. The Ku potein plays a key ole in multiple nuclea pocesses, e.g., DNA epai, chomosome maintenance, tansciption egulation, and V(D)J ecombination. The mechanism undelying the egulation of all the divese functions of Ku is still unclea, although it seems that Ku is a multifunctional potein that woks in nuclei. On the othe hand, seveal studies have epoted cytoplasmic o cell suface localization of Ku in vaious cell types. To claify the fundamental chaacteistics of Ku, we have examined the expession, heteodimeization, subcellula localization, chomosome location, and molecula mechanisms of the nuclea tanspot of Ku70 and Ku80. The mechanism that egulates fo nuclea localization of Ku70 and Ku80 appeas to play, at least in pat, a key ole in egulating the physiological function of Ku in vivo.
Journal of Radiation Research2002Volume 43, Issue 3, SeptemberDrinking Beer Reduces Radiation-induced Chromosome Aberrations in Human Lymphocyteshttps://doi.org/10.1269/jrr.43.237Journal of Radiation Research, Volume 43, Issue 3, September 2002, Pages 237–245,Manami Monobe; Koichi Ando*2002/9/1Manami Monobe 1Graduate School of Science and Technology, Chiba University, 1–33, Yayoi-cho, Inage-ku, Chiba 263–8522, Japan2Division of Heavy-ion Radiobiology Research Group, National Institute of Radiological Sciences, 9–1, Anagawa-4-chome, Inage-ku, Chiba 263–8555, Japan; Koichi Ando 2Division of Heavy-ion Radiobiology Research Group, National Institute of Radiological Sciences, 9–1, Anagawa-4-chome, Inage-ku, Chiba 263–8555, Japan Corresponding author: Phone: +81–43–206–3231, Fax: +81–43–206–4149, E-mail: ando@nirs.go.jp"237-245"We hee investigated and epoted the effects of bee dinking on adiation-induced chomosome abeations in blood lymphocytes. Human blood that was collected eithe befoe o afte dinking a 700 ml bee was in vito iadiated with 200 kVp X ays o 50 keV/μm cabon ions. The elation between the adiation dose and the abeation fequencies (fagments and dicentics) was significantly (p ?? 0.05) lowe fo lymphocytes collected 3 h afte bee dinking than those befoe dinking. Fitting the dose esponse to a linea quadatic model showed that the alpha tem of cabon ions was significantly (p ?? 0.05) deceased by bee dinking. A decease of dicentic fomation was detected as ealy as 0.5 h afte bee dinking, and lasted not shote than 4.5 h. The mitotic index of lymphocytes was highe afte bee dinking than befoe, indicating that a division delay would not be esponsible fo the low abeations induced by bee dinking. An in vito teatment of nomal lymphocytes with 0.1 M ethanol, which coesponded to a concentation of 6-times highe than the maximum ethanol concentation in the blood afte bee dinking, educed the dicentic fomation caused by X-ay iadiation, but not by cabon-ion iadiation. The bee-induced eduction of dicentic fomation was not affected by seum. It is concluded that bee could contain non-ethanol elements that educe the chomosome damage of lymphocytes induced by high-LET adiation.
Journal of Radiation Research2002Volume 43, Issue 3, SeptemberRelative Biological Effectiveness of 290 MeV/u Carbon Ions for the Growth Delay of a Radioresistant Murine Fibrosarcomahttps://doi.org/10.1269/jrr.43.247Journal of Radiation Research, Volume 43, Issue 3, September 2002, Pages 247–255,Sachiko Koike*; Koichi Ando; Chisa Oohira; Takeshi Fukawa; Ryonfa Lee; Nobuhiko Takai; Manami Monobe; Yoshiya Furusawa; Mizuho Aoki; Shigeru Yamada; Wakako Shimizu; Kumie Nojima; Hideyuki Majima2002/9/1Sachiko Koike 1Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba 263–8555, Japan Corresponding author: Phone: +81–43–251–2111 ext. 6921, Fax: +81–43–206–4149, E-mail: koike@nirs.go.jp; Koichi Ando 1Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba 263–8555, Japan; Chisa Oohira 1Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba 263–8555, Japan; Takeshi Fukawa 1Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba 263–8555, Japan; Ryonfa Lee 1Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba 263–8555, Japan; Nobuhiko Takai 1Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba 263–8555, Japan; Manami Monobe 1Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba 263–8555, Japan; Yoshiya Furusawa 1Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba 263–8555, Japan; Mizuho Aoki 1Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba 263–8555, Japan; Shigeru Yamada 1Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba 263–8555, Japan; Wakako Shimizu 1Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba 263–8555, Japan; Kumie Nojima 1Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba 263–8555, Japan; Hideyuki Majima 1Heavy-Ion Radiobiology Research Group, National Institute of Radiological Sciences, 4–9–1 Anagawa, Inage-ku, Chiba 263–8555, Japan"247-255"The elative biological effectiveness (RBE) fo animal tumos teated with factionated doses of 290 MeV/u cabon ions was studied. The gowth delay of NFSa fibosacoma in mice was investigated following vaious daily doses given with cabon ions o those given with cesium γ-ays, and the RBE was detemined. Animal tumos wee iadiated with cabon ions of vaious LET (linea enegy tansfe) in a 6-cm SOBP (spead-out Bagg peak), and the isoeffect doses; i.e. the dose necessay to induce a tumo gowth delay of 15 days wee studied. The isoeffect dose fo cabon ions of 14 and 20 keV/μm inceased with an incease in the numbe of factions up to 4 factions. The incease in the isoeffect dose with the faction numbe was small fo cabon ions of 44 keV/μm, and was not obseved fo 74 keV/μm. The α and β values of the linea-quadatic model fo the adiation dose-cell suvival elationship wee calculated by the Fe-plot analysis method. The α values inceased linealy with an incease in the LET, while the β values wee independent of the LET. The α/β atio was 129 ± 10 Gy fo γ-ays, and inceased with an incease in the LET, eaching 475 ± 168 Gy fo 74 keV/μm cabon ions. The RBE fo cabon ions elative to Cs-137 γ-ays inceased with the LET. The RBE values fo 14 and 20 keV/μm cabon ions wee 1.4 and independent of the numbe of factions, while those fo 44 and 74 keV/μm inceased fom 1.8 to 2.3 and fom 2.4 to 3.0, espectively, when the numbe of factions inceased fom 1 to 4. Inceasing the numbe of factions futhe fom 4 to 6 was not associated with an incease in the RBE. These esults togethe with ou ealie study on the skin eaction suppot the use of an RBE of 3.0 in clinical tials of 80 keV/μm cabon beams. The RBE values fo low doses of cabon beams wee also consideed.
Journal of Radiation Research2002Volume 43, Issue 3, SeptemberModels for Mixed Irradiation with a ‘Reciprocal-Time’ Pattern of the Repair Functionhttps://doi.org/10.1269/jrr.43.257Journal of Radiation Research, Volume 43, Issue 3, September 2002, Pages 257–267,Shozo Suzuki*; Yuri Miura; Shoichi Mizuno; Yoshiya Furusawa2002/9/1Shozo Suzuki 1Redox Regulation Research Group, Tokyo Metropolitan Institute of Gerontology, Chiba 263–8555, Japan Corresponding author: Phone:+81–3–3964–3241 ext. 3142, Fax: +81–3–3579–4776, E-mail: shozo@tmig.or.jp; Yuri Miura 1Redox Regulation Research Group, Tokyo Metropolitan Institute of Gerontology, Chiba 263–8555, Japan; Shoichi Mizuno 1Redox Regulation Research Group, Tokyo Metropolitan Institute of Gerontology, Chiba 263–8555, Japan; Yoshiya Furusawa 1Redox Regulation Research Group, Tokyo Metropolitan Institute of Gerontology, Chiba 263–8555, Japan"257-267"Suzuki pesented models fo mixed iadiation with two and multiple types of adiation by extending the Zaide and Rossi model, which is based on the theoy of dual adiation action. In these models, the epai function was simply assumed to be semi-logaithmically linea (i.e., monoexponential), o a fist-ode pocess, which has been expeimentally contadicted. Fowle, howeve, suggested that the epai of adiation damage might be lagely a second-ode pocess athe than a fist-ode one, and pesented data in suppot of this hypothesis. In addition, a second-ode epai function is pefeed to an n-exponential epai function fo the eason that only one paamete is used in the fome instead of 2n-1 paametes fo the latte, although both epai functions show a good fit to the expeimental data. Howeve, accoding to a second-ode epai function, the epai ate depends on the dose, which is incompatible with the expeimental data. We, theefoe, evised the models fo mixed iadiation by Zaide and Rossi and by Suzuki, by substituting a ‘ecipocal-time’ patten of the epai function, which is deived fom the assumption that the epai ate is independent of the dose in a second-ode epai function, fo a fist-ode one in eduction and inteaction factos of the models, although the undelying mechanism fo this assumption cannot be well-explained. The eduction facto, which educes the contibution of the squae of a dose to cell killing in the linea-quadatic model and its deivatives, and the inteaction facto, which also educes the contibution of the inteaction of two o moe doses of diffeent types of adiation, wee fomulated by using a ‘ecipocal-time’ patten of the epai function. Cell suvivals calculated fom the olde and the newly modified models wee compaed in tems of the dose-ate by assuming vaious types of single and mixed iadiation. The esult implies that the newly modified models fo mixed iadiation can expess o pedict cell suvival moe accuately than the olde ones, especially when iadiation is polonged at low dose ates.
Journal of Radiation Research2002Volume 43, Issue 3, SeptemberEffects of Heavy-ion Radiosurgery on the Hemopoietic Function of the Silkworm Bombyx morihttps://doi.org/10.1269/jrr.43.269Journal of Radiation Research, Volume 43, Issue 3, September 2002, Pages 269–275,Zhen-Li Tu*; Yasuhiko Kobayashi; Kenji Kiguchi; Hiroshi Watanabe; Kazuo Yamamoto2002/9/1Zhen-Li Tu 1Biotechnology Laboratory, Department of Radiation Research for Environment and Resources, Takasaki Radiation Chemistry Research Establishment, Japan Atomic Energy Research Institute (JAERI), Watanukimachi 1233, Takasaki, Gunma 370–1292, Japan Corresponding author: Phone: 81–027–346–9542, Fax: 81–027–346–9688, E-mail: tu514@yahoo.co.jp; Yasuhiko Kobayashi 1Biotechnology Laboratory, Department of Radiation Research for Environment and Resources, Takasaki Radiation Chemistry Research Establishment, Japan Atomic Energy Research Institute (JAERI), Watanukimachi 1233, Takasaki, Gunma 370–1292, Japan; Kenji Kiguchi 1Biotechnology Laboratory, Department of Radiation Research for Environment and Resources, Takasaki Radiation Chemistry Research Establishment, Japan Atomic Energy Research Institute (JAERI), Watanukimachi 1233, Takasaki, Gunma 370–1292, Japan; Hiroshi Watanabe 1Biotechnology Laboratory, Department of Radiation Research for Environment and Resources, Takasaki Radiation Chemistry Research Establishment, Japan Atomic Energy Research Institute (JAERI), Watanukimachi 1233, Takasaki, Gunma 370–1292, Japan; Kazuo Yamamoto 1Biotechnology Laboratory, Department of Radiation Research for Environment and Resources, Takasaki Radiation Chemistry Research Establishment, Japan Atomic Energy Research Institute (JAERI), Watanukimachi 1233, Takasaki, Gunma 370–1292, Japan"269-275"To study the effects of heavy-ion adiosugey on the hemopoietic function of a silkwom, hemopoietic ogans of lavae wee locally iadiated with cabon-ion beams, and the changes in the hemocyte density and in the hemocyte function wee investigated. When the lavae wee iadiated by 50 Gy to 300 Gy cabon ions on the 3d day of the 4th insta, the hemocyte densities did not change fo a while, though they gadually inceased at a late stage, but wee finally still significantly lowe than those of uniadiated contols. The hemocyte densities of the lavae iadiated at diffeent developmental stages showed suppessed incements, and cabon-ion iadiation given to lavae at ealy stages compaed to the late stages had a significant suppessive effect on the hemocyte densities. On unilateal iadiated lavae a hemocyte intemediate incement between those of bilateal iadiated lavae and uniadiated contols was obseved. The pecentage of dead hemocytes was obviously highe fo iadiated lavae than uniadiated contols duing the late 5th insta. Thus, it is evident that cabon-ion adiosugey on hemopoietic ogans of silkwom induced not only a quantitative change, but also a qualitative change in the hemocytes.
Journal of Radiation Research2002Volume 43, Issue 3, SeptemberChanges in Histological Construction and Decrease in 3H-QNB Binding in the Rat Brain after Prenatal X-irradiationhttps://doi.org/10.1269/jrr.43.277Journal of Radiation Research, Volume 43, Issue 3, September 2002, Pages 277–282,Sotaro Momosaki; Xue-Zhi Sun; Nobuhiko Takai; Rie Hosoi; Osamu Inoue; Sentaro Takahashi*2002/9/1Sotaro Momosaki 1Department of Medical Physics, School of Allied Health Sciences, Faculty of Medicine, Osaka University, 1–7 Yamada-oka, Suita, Osaka 565–0871, Japan; Xue-Zhi Sun 1Department of Medical Physics, School of Allied Health Sciences, Faculty of Medicine, Osaka University, 1–7 Yamada-oka, Suita, Osaka 565–0871, Japan; Nobuhiko Takai 1Department of Medical Physics, School of Allied Health Sciences, Faculty of Medicine, Osaka University, 1–7 Yamada-oka, Suita, Osaka 565–0871, Japan; Rie Hosoi 1Department of Medical Physics, School of Allied Health Sciences, Faculty of Medicine, Osaka University, 1–7 Yamada-oka, Suita, Osaka 565–0871, Japan; Osamu Inoue 1Department of Medical Physics, School of Allied Health Sciences, Faculty of Medicine, Osaka University, 1–7 Yamada-oka, Suita, Osaka 565–0871, Japan; Sentaro Takahashi 1Department of Medical Physics, School of Allied Health Sciences, Faculty of Medicine, Osaka University, 1–7 Yamada-oka, Suita, Osaka 565–0871, Japan Corresponding author: Phone: 043–206–3158, Fax: 043–251–4853, E-mail: sentaro@nirs.go.jp"277-282"To elucidate the mechanisms involved in deleteious neuonal and behavioal changes afte penatal ionizing iadiation, in vito muscainic acetylcholine (mACh) ecepto binding and histological constuction wee investigated in 9-week old at bains afte 1.5 Gy X-ay exposue on embyonic day 15 (E15). A goss anatomical examination with a magnetic-esonance imaging system showed an iegula tissue constuction in the hippocampus and cotex of the iadiated at bain. Histological sections stained with hematoxylin and eosin also indicated that the stuctues of the hippocampus and cotex wee obviously changed. In iadiated ats, the lamina stuctue of pyamidal cells was selectively deanged in the CA1 egion. In vito3H-Quinuclidinyl benzilate binding in the hippocampus was significantly deceased (about 10%) in penatal iadiated ats compaed to that in sham-teated ats. On the othe hand, no significant change in mACh ecepto binding was obseved in the ceebal cotex. The pesent study evealed that penatal exposue to ionizing adiation may induce dysfunction of the cholinegic neuonal systems, especially in the hippocampus, esulting in deleteious changes in memoy and behavio.
Journal of Radiation Research2002Volume 43, Issue 3, SeptemberRadiosensitization by Overexpression of the Nonphosphorylation Form of IκB-α in Human Glioma Cellshttps://doi.org/10.1269/jrr.43.283Journal of Radiation Research, Volume 43, Issue 3, September 2002, Pages 283–292,Naoko Honda; Kasumi Yagi; Gui-Rong Ding; Junji Miyakoshi*2002/9/1Naoko Honda 1Department Radiation Genetics, Graduate School of Medicine, Yoshida-Konoe-cho, Sakyo-Ku, Kyoto 606–8501, Japan; Kasumi Yagi 1Department Radiation Genetics, Graduate School of Medicine, Yoshida-Konoe-cho, Sakyo-Ku, Kyoto 606–8501, Japan2Department of Zoology, Graduate School of Science, Kyoto University, Yoshida-Konoe-cho, Sakyo-Ku, Kyoto 606–8501, Japan; Gui-Rong Ding 1Department Radiation Genetics, Graduate School of Medicine, Yoshida-Konoe-cho, Sakyo-Ku, Kyoto 606–8501, Japan3Department of Radiation Medicine, Fourth Military Medical University, Xi'an 710032, China; Junji Miyakoshi 1Department Radiation Genetics, Graduate School of Medicine, Yoshida-Konoe-cho, Sakyo-Ku, Kyoto 606–8501, Japan Corresponding author: Phone; +81–75–753–4666, Fax; +81–75–753–4669, E-mail, miyakosh@mfour.med.kyoto-u.ac.jp"283-292"To assess the ole of NF-κB in cellula adiosensitivity, we constucted mutated IκB expession plasmids fo SY-IκB (with mutations at esidues of 32, 36 and 42) expession in human malignant glioma cells (adiosensitive MO54 and adioesistant T98 cells), giving espective cell types efeed to as MO54-SY4 and T98-SY14. Both of the clones expessing SY-I κB became adiosensitive, compaed with the paental MO54 and T98 cells. A teatment with hebimycin A o genistein did not change the adiosensitivity of cells expessing SY-IκB, but made both the MO54 and T98 paental cells moe sensitive to ionizing adiation. A teatment with TNF-α induced DNA fagmentation and apoptosis in cells expessing SY-IκB, but not in MO54 and T98 cells. The suvival afte X-ay exposue of the paental MO54 cells was slightly inceased by a TNF-α teatment, but that of the paental T98 cells did not change. The change in sensitivity to ulta-violet (UV) adiation and adiamycin in MO54-SY4 cells was vey simila to that fo X-ay sensitivity, but no change was obseved in T98-SY14 cells. Significant sublethal damage epai was obseved in T98 cells, wheeas MO54 cells showed little epai activity. The expession of p53 was enhanced in the paental MO54 cells, while the p53 levels in the MO54-SY4, and in the paent and clonal T98 cells, did not change. Ou data suggest that the seine and tyosine phosphoylation of IκB-α may play a ole in detemining the adiosensitivity of malignant glioma cells.
Journal of Radiation Research2002Volume 43, Issue 3, SeptemberRadioprotective Effects of 2-Imino-3-(chromone-2-yl)carbonyl thiazolidines against γ-Irradiation in Micehttps://doi.org/10.1269/jrr.43.293Journal of Radiation Research, Volume 43, Issue 3, September 2002, Pages 293–300,Seyed Jalal Hosseinimehr; Abbas Shafiee*; Hossein Mozdarani; Shahram Akhlagpour; Mohsen Froughizadeh2002/9/1Seyed Jalal Hosseinimehr 1Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran; Abbas Shafiee 1Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran Corresponding author: Tel.: +98–21–6406757; Fax: +98–21–6461178; E-mail: ashafiee@ams.ac.ir; Hossein Mozdarani 1Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran; Shahram Akhlagpour 1Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran; Mohsen Froughizadeh 1Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran"293-300"A seies of 2-imino-3-(chomone-2-yl) cabonylthiazolidines substituted at the C-5 and / o C-7 positions of a chomone ing wee synthesized. The in vivo toxicity and adiopotective efficacy of these agents wee evaluated in male NMRI mice against cobalt-60 γ-ays. The LD50 values as detemined by a Pobit analysis, wee 659, 1216 and 790 mg/kg fo compounds, 2, 3 and 4, espectively. Fo studying adiopotective effects, one half of the toxic LD50 values wee used, namely 330, 605 and 395 mg/kg fo compounds 2, 3 and 4, espectively. The dose educed facto (DRF) was detemined by dividing the LD50/30 values obtained fom the adiation suvival cuve in the pesence of a adiopotective agent by the LD50/30 value obtained fom a contol adiation suvival cuve. A compound with a hydoxyl goup substituent at the C-5 position affoded bette adiopotective activity than those without this substituent. The adiopotective effect of chomone having a hydoxyl goup at only the C-7 position was simila to that of the unsubstituted chomone. The most active compound has hydoxyl goups at the C-5 and C-7 positions of the chomone ing; it had a DRF of 1.48.

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